Embryo culture device

Abstract

The invention discloses an embryo culture device, which comprises: at least one embryo culture module capable of independently culturing; the embryo culture module comprises a culture chamber and a culture assembly; and the culture chamber comprises a top cover with a cavity formed in the lower portion and a culture platform arranged in the cavity in a sliding mode, and a sealed culture space used for containing the culture assembly can be formed between the top cover and the culture platform. The culture assembly comprises a culture dish arranged on the culture platform, at least one embryo cover arranged in the culture dish, and a liquid inlet needle and a liquid discharge needle which are respectively arranged on two sides of the embryo cover. The automatic liquid changing function in the embryo culture process can be achieved, and the influence of repeated box opening and liquid changing operation on embryos can be avoided; the embryo culture chamber adopts a pull-out bin gate structure, and can be conveniently matched with an optical monitoring system to realize online monitoring; and during liquid changing operation, embryos can be effectively restrained from being separated from the optical view field, online automatic liquid changing can be supported, and dynamic culture can be achieved.

Description

technical field [0001] The invention relates to the field of embryo culture in vitro, in particular to an embryo culture device. Background technique [0002] Birth defects have always been an important problem in the field of reproductive health. According to reports, the total incidence of birth defects in my country is about 5.6%. The diseases mainly include structural deformities such as congenital heart disease and cleft lip and palate, as well as genetic diseases such as phenylketonuria and severe spinal muscular atrophy. Birth defects can occur at any stage of pregnancy, especially early. With the full opening of my country's second-child policy in 2015, the prevention and treatment of birth defects has become an important research direction in the field of reproductive biology. But at present, there are still many unsolved mysteries about the physiological process of the normal development of human embryos. For example, the dynamic changes of X chromosome inactiva...

AI Technical Summary

Problems solved by technology

[0003] At present, in the field of assisted reproduction, the in vitro culture technology of embryos is gradually mature, and the common desktop or box incubators can only achieve basic culture functions, that is, they can provide basic environments such as temperature, humidity and CO2 gas mixture, but cannot perform real-time optical incubators. Monitoring, observing the details of embryonic development, recording morphological changes during embryonic development, or capturing dynamic images that may appear in the later stages of embryonic development

During the long-term continuous development of embryos, the demand for nutrients in the culture medium is dynamically changing, and the embryos themselves will slowly release some metabolites, which need to be removed in time, and the current incubator needs to take out the petri dish for replacement. Liquid manipulation will inevitably have a potential impact on embryonic development

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