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Beauveria bassiana preparation and preparation method and use method thereof

A technology of Beauveria bassiana and preparations, which is applied in the field of Beauveria bassiana preparations and its preparation, which can solve the problems of spore germination and growth of hyphae infecting insect bodies, limitation of Beauveria bassiana effect, low persistence, etc. question

Inactive Publication Date: 2021-05-04
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the use of Beauveria bassiana in the prior art to control corn borers still faces some problems. For example, when applied in the field, the field environment is mostly a low-humidity and high-temperature environment, which is not conducive to spore germination and growth of mycelium to infect the insect body. Factors such as exposure time and light time will greatly restrict the application of Beauveria bassiana in the field and its effect.
Although the patent application No. 201610578800.0 has provided a biological agent of Beauveria bassiana that can adapt to the field environment, the persistence of the biological agent in the stem and leaf parts of the ground where the corn borer is mainly damaged is not high

Method used

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  • Beauveria bassiana preparation and preparation method and use method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Beauveria bassiana strain GZ01 was inoculated in PDA medium and cultured at 25°C in the dark for 10 days to obtain complete sporogenous Beauveria bassiana. The Beauveria bassiana preparation of Example 1 was prepared by scraping the Beauveria bassiana with a knife and mixing the culture medium and corn stalks at a weight ratio of 1:1.

Embodiment 2

[0042] Beauveria bassiana strain GZ01 was inoculated in PDA medium and cultured at 27°C in the dark for 7 days to obtain complete sporogenous Beauveria bassiana. The Beauveria bassiana preparation of Example 2 was prepared by scraping the Beauveria bassiana with a knife and mixing the culture medium and corn stalks at a weight ratio of 1:2.

experiment example 1

[0044] The experimental area was divided into experimental group and control group, with 3 replicates in each group. The Beauveria bassiana preparation prepared in Example 1 was placed in a container carried by an unmanned aerial vehicle under dark conditions at 25° C. for 3 days, and sprayed on each test area of ​​the experimental group. The spraying rate is 0.5kg / mu. The flying height of the drone is 1.2m above the canopy of the corn plant, the flying speed is 8m / s, the working range is 4.5m, and the spraying is carried out at an interval of 1.5m between each flight track.

[0045] On the day of spraying Beauveria bassiana preparation, 5 points were randomly selected in each test area, the distance between each point was 5m, and 20 corn plants were randomly selected at each point, that is, 100 corn plants were randomly selected in each test area for artificial inoculation. The inoculation method is as follows: after the artificially reared Asian corn borer adults lay eggs, ...

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Abstract

The invention relates to the technical field of agricultural pest control, in particular to a beauveria bassiana preparation and a preparation method and a use method thereof. The preparation method comprises the following steps that (1), a beauveria bassiana strain is inoculated into a PDA culture medium for culturing to obtain spore-producing beauveria bassiana; and (2), the spore-producing beauveria bassiana obtained in the step (1) is mixed with a culture medium and corn straw to obtain the beauveria bassiana preparation. The beauveria bassiana preparation can adapt to a field environment, further can enable the overground part of corn to have high persistence of the beauveria bassiana, so that a better effect of preventing and treating corn borers is achieved.

Description

technical field [0001] The invention relates to the technical field of agricultural pest control, in particular to a Beauveria bassiana preparation, a preparation method and a use method thereof. Background technique [0002] The Asian corn borer, Ostrinia furnacalis, belongs to Lepidoptera and Mothidae. It is widely distributed in most countries in Asia and Oceania. In my country, the Asian corn borer is widely distributed from northeast to Hainan. It is the main pest in corn production in my country and seriously affects the yield and quality of corn. The corn borer mainly damages the aboveground parts of the corn plant, causing the damaged parts to lose their function, reducing the grain yield and the commerciality of fresh ears. [0003] Beauveria bassiana is an important entomopathogenic fungus that has been widely studied and applied. It has a wide range of hosts and can parasitize more than 700 species of insects from 15 orders. It can not only parasitize insects, b...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/30A01P7/04A01G13/00A01M7/00C12R1/645
CPCA01G13/00A01M7/00A01N63/30C12N1/14
Inventor 汪洋洲张云月杜茜高月波孙嵬常雪周淑香李丽娟庞建成张国红张强周佳春丁岩
Owner JILIN ACAD OF AGRI SCI
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