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Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding

A frequency space, imaging system technology, applied in the field of biological fluorescence imaging

Active Publication Date: 2021-05-18
SOUTH CHINA UNIV OF TECH
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Problems solved by technology

The laser emitting device is used to emit a pulsed excitation beam

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  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding
  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding
  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding

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[0035] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the application are given in the drawings. However, the present application can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of this application more thorough and comprehensive.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used herein in the specification of the application are only for the purpose of describing specific embodiments, and are not intended to limit the application.

[0037] It can be understood that the terms "first", "second" and the like used in this application may be...

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Abstract

The invention relates to a multiphoton excitation imaging system andimaging method based on two-dimensional frequency space coding. The multiphoton excitation imaging system is characterized in that a two-dimensional deflection device forms a two-dimensional frequency space coding laser beam dot matrix by changing the deflection angle of a first excitation light beam; in the two-dimensional frequency space coding laser beam dot matrix, radio frequency offset exists between the light beam carrier frequency corresponding to each laser beam point and the light beam carrier frequency corresponding to other laser beam points; a frequency shift device performs frequency shift on the carrier frequency of a second excitation light beam so as to enable the frequency offset of the frequency of the second excitation light beam to be within the frequency offset in the radio frequency band; and the frequency of the second excitation light beam can be adjusted by the frequency shift device, and the effect of restraining low frequency and zero frequency is achieved. Through utilizing the combined light beam formed by the two-dimensional frequency space coding laser beam dot matrix and a frequency shift light beam, dot-matrix excitation of fluorophores at different positions in a sample can be realized, and the problem of low imaging speed of a traditional biological fluorescence imaging system is solved.

Description

technical field [0001] The invention relates to the technical field of biological fluorescence imaging, in particular to a multi-photon excitation imaging system and imaging method based on two-dimensional frequency space coding. Background technique [0002] Bioluminescent imaging technology has a wide range of applications in the field of biomedicine, such as obtaining cell morphology, cytochemical properties, and cell material distribution characteristics, and studying the dynamic process of intracellular and intercellular material exchange. The bioluminescent imaging process reconstructs the fluorescence image of biological samples by exciting fluorophores at different positions of the biological sample and collecting the fluorescent signals at the corresponding positions. [0003] However, traditional bioluminescent imaging systems are still challenging to capture the high-speed dynamic process of fluorophore-labeled substances in biological samples. Traditional biolum...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6486G01N2021/6419G01N2021/6463
Inventor 杨中民韦小明王文龙
Owner SOUTH CHINA UNIV OF TECH
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