Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding

A technology of frequency space and imaging system, applied in the field of bioluminescence imaging

Active Publication Date: 2022-02-15
SOUTH CHINA UNIV OF TECH
View PDF11 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The laser emitting device is used to emit a pulsed excitation beam

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding
  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding
  • Multiphoton excitation imaging system and imaging method based on two-dimensional frequency space coding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the relevant drawings. Embodiments of the application are given in the drawings. However, the present application can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of this application more thorough and comprehensive.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field to which this application belongs. The terms used herein in the specification of the application are only for the purpose of describing specific embodiments, and are not intended to limit the application.

[0037] It can be understood that the terms "first", "second" and the like used in this application may be...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a multi-photon excitation imaging system and imaging method based on two-dimensional frequency space encoding. A two-dimensional deflection device forms a two-dimensional frequency space encoding laser beam lattice by changing the deflection angle of the first excitation beam. In the two-dimensional frequency space coded laser beam lattice, there is a radio frequency offset between the beam carrier frequency corresponding to each laser beam spot and the beam carrier frequency corresponding to other laser beam spots. The frequency shifting device performs a frequency shift on the carrier frequency of the second excitation light beam, so as to make the frequency shift amount of the frequency of the second excitation light beam within the frequency shift amount within the radio frequency band. The frequency of the second excitation light beam can be adjusted by the frequency shifting device to suppress low frequency and zero frequency. The combined beam formed by the two-dimensional frequency space coded laser beam lattice and the frequency-shifted beam can realize lattice excitation of fluorophores at different positions in the sample, which solves the problem of low imaging speed of traditional bioluminescent imaging systems. question.

Description

technical field [0001] The invention relates to the technical field of biological fluorescence imaging, in particular to a multi-photon excitation imaging system and imaging method based on two-dimensional frequency space coding. Background technique [0002] Bioluminescent imaging technology has a wide range of applications in the field of biomedicine, such as obtaining cell morphology, cytochemical properties, and cell material distribution characteristics, and studying the dynamic process of intracellular and intercellular material exchange. The bioluminescent imaging process reconstructs the fluorescence image of biological samples by exciting fluorophores at different positions of the biological sample and collecting the fluorescent signals at the corresponding positions. [0003] However, traditional bioluminescent imaging systems are still challenging to capture the high-speed dynamic process of fluorophore-labeled substances in biological samples. Traditional biolum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486G01N2021/6419G01N2021/6463
Inventor 杨中民韦小明王文龙
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com