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Separation and primary culture method of vascular sac cells of Lateolabrax maculatus

A technology of primary culture and vascular capsule, applied in cell dissociation methods, biochemical equipment and methods, tissue culture, etc.

Pending Publication Date: 2021-06-08
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no information related to the isolation and primary culture of the perch vascular sac cells at home and abroad. Therefore, it is necessary to establish a standardized and reproducible method for the isolation and primary culture of the perch vascular sac cells for the seasonal reproduction of the perch. Research provides an experimental platform

Method used

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  • Separation and primary culture method of vascular sac cells of Lateolabrax maculatus
  • Separation and primary culture method of vascular sac cells of Lateolabrax maculatus
  • Separation and primary culture method of vascular sac cells of Lateolabrax maculatus

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Embodiment Construction

[0023] The technical solutions of the present invention will be further described and described in connection with the accompanying drawings in conjunction with the accompanying drawings.

[0024] In the following examples, DPBS Basic (1x) buffer, L-15 base medium, penicillin streptomycin double-binding in GIBCO, USA; fetal bovine serum FBS purchased in GIBCO, pancreatic disintegration, hosted in Biosharp.

[0025] Preparation solution

[0026] Double anti-DPBS: Add penicillin and streptomycin double resistance to 400u / ml in DPBS.

[0027] Double Anti-L-15 Media: Add penicillin streptomycin double resistance to 400 U / mL in the L-15 basic medium.

[0028] L-15 Complete Medium 1: Adding penicillin double anti-double anti-400U / ml is added to the L-15 base medium, and 20% FBS is added at the same time.

[0029] L-15 Complete Medium 2: Add penicillin streptomycin double resistance to 400 U / mL in the L-15 base medium, and add 10% FBS.

[0030] L-15 Complete Medium 3: Adding peni...

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Abstract

The invention discloses a separation and primary culture method of vascular sac cells of Lateolabrax maculatus. The method comprises the following steps: taking a complete vascular sac tissue, rinsing the tissue with a buffer solution and a culture medium, cutting the tissue into not less than 3 blocks, placing the blocks in a well-plate, and carrying out standing culture under optimized culture conditions. The method for separation adopted by the invention can reduce cell loss and cell morphological damage during operation and washing. The method for primary culture adopted by the invention can allow a large number of primary culture cells to adhere to wall, and has a good passage effect.

Description

Technical field [0001] The present invention relates to the culture of fish cells, and specifically, there is a separation and primary culture method of flower blood vesicle cells. [0002] technical background [0003] Lateolabrax Maculatus Hardbone Fish (Osteichthyes), Actinopterygii, Fragiform (PerciDei), SERCOIDEI, Siko (serran), Lateolabrax. Huatu is widely distributed in China, North Korea and Japanese coast, has a wide range of water, strong salt, delicious meat, high economic efficiency, is an important aquaculture variety in my country. [0004] Flower is a short-term seasonal propagation fish, the northern spawning period is October-December, the south is November - January of the following year. Seasonal propagation fish will appear a photopolymerization and thyroxine will participate in seasonal reproductive regulation. Due to a series of problems caused by flowering of fluttering righteousness in winter, low temperature caused by low temperature, low, and higher bait ...

Claims

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Application Information

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IPC IPC(8): C12N5/071
CPCC12N5/0602C12N2509/00
Inventor 王鹏飞邱丽华闫路路赵超范嗣刚郑少华袁满
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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