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Method for detecting natural estrogen in soil and sediment

A detection method and sediment technology, which is applied in the field of detection of natural estrogen substances in soil and sediment, to achieve the effect of efficient detection of extraction efficiency and high accuracy

Inactive Publication Date: 2021-06-11
上海国齐检测技术有限公司
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  • Claims
  • Application Information

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Problems solved by technology

[0004] The object of the present invention is to provide a kind of detection method of natural estrogen in soil and sediment, to solve the detection and analysis of natural estrogen in soil and sediment in the country and environmental monitoring industry proposed in the above-mentioned background technology method problem

Method used

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  • Method for detecting natural estrogen in soil and sediment
  • Method for detecting natural estrogen in soil and sediment

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Embodiment 1

[0034] Embodiment 1: a kind of detection method of natural estrogen in soil and sediment, comprises the following steps:

[0035] Step 1: Freeze the collected soil and sediment samples in a refrigerator at 4°C for 24 hours;

[0036] Step 2: Take out the sample and quickly carry out vacuum freeze-drying for 2 hours;

[0037] Step 3: Weigh 1.0±0.1g sample into a 10ml centrifuge tube, add internal standard solution, the internal standard is 17β-estradiol-D3, the added concentration is 50ng / kg, add 5.0ml dichloromethane and methanol to mix The liquid was subjected to ultrasonic extraction for 25 min;

[0038] Step 4: Collect the extract after centrifugation;

[0039] Step 5: Add 2.0ml of dichloromethane and methanol mixture for ultrasonic extraction for 25min;

[0040] Step 6: Collect the extract after centrifugation, and combine the extracts from the two ultrasonic extractions;

[0041] Step 7: The extract was heated in a water bath at 45°C and concentrated to 0.5ml by nitrog...

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Abstract

The invention discloses a method for detecting natural estrogen in soil and sediments, relates to the field of methods for detecting natural estrogen substances in soil and sediments in the field of environmental detection, and aims to solve a problem that a method for detecting and analyzing natural estrogen substances in soil and sediments does not exist in the industry. The method comprises the following steps of 1, freezing a sample; 2, carrying out vacuum freeze drying; 3, carrying out ultrasonic extraction; 4, collecting extract liquor after centrifugation; 5, carrying out ultrasonic extraction; 6, collecting extract liquor after centrifugation, and combining the extract liquor obtained by two times of ultrasonic extraction; 7, heating the extract liquid in a water bath, and carrying out nitrogen blowing concentration and constant volume; 8, performing extraction; 9, carrying out column activation by using normal hexane, eluting, and blow-drying by using a nitrogen blowing instrument; 10, adding a derivatization reagent, carrying out microwave derivatization, and fixing the volume; 11, checking the performance of the instrument; 12, drawing a standard solution; and 13, determining a result.

Description

technical field [0001] The invention relates to the field of detection methods for natural estrogen substances in soil and sediments in the field of environmental detection, in particular to a detection method for natural estrogen substances in soil and sediments. Background technique [0002] Natural estrogen substances mainly exist in various types of aquaculture. According to many surveys, farmers will use estrogen substances in the process of breeding to improve the reproduction and production of species. However, estrogen is harmful to the human endocrine system. Metabolism, growth and development have a great impact; solid waste containing estrogen is difficult to degrade when it enters the soil and sediment, and it is a kind of persistent organic pollutants. In recent years, the detection technology of estrogen has been greatly improved than before, mainly electrochemical analysis method, chromatography and immunological method, etc. The chromatography detection metho...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/045
Inventor 姬丹金勇沈欢
Owner 上海国齐检测技术有限公司
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