Construction method and device of depression-induced breast cancer animal model and application

An animal model and construction method technology, applied in the biological field, can solve the problems of high animal mortality, large individual differences, and long modeling time, and achieve the effects of low animal mortality, small individual differences, and short modeling time.

Active Publication Date: 2021-07-13
GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, due to various technical difficulties, there is still a lack of an animal model that can simulate the mechanism of depression-induced breast tumors. At the same time, due to the complicated operation, poor reproducibility, and large individual differences of the animal models used in research on depressive disorders , long modeling time, high animal mortality and other shortcomings, it cannot be directly applied to animal models that simulate induced breast cancer

Method used

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  • Construction method and device of depression-induced breast cancer animal model and application
  • Construction method and device of depression-induced breast cancer animal model and application
  • Construction method and device of depression-induced breast cancer animal model and application

Examples

Experimental program
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Effect test

Embodiment 1

[0050] see Figure 1-12 , the method for constructing the depression-induced breast cancer animal model provided by the embodiments of the present invention, which comprises the following steps:

[0051] (1) Cultivate 5-week-old FVB mice with MMTV-PyMT transgenic spontaneous breast cancer as experimental animals, and divide them into control group and model group;

[0052] (2) Prepare a restraint device that causes depression in mice;

[0053] (3) Raise the mice in the model group under the set obstacle conditions: each mouse is independently placed in an indoor restraint device, and its body is mechanically pressed in the restraint device, unable to turn its head and turn around, unable to Movement, bound for 4 hours a day, maintaining a 12-hour light-dark cycle, which lasted for 3 weeks or more, made them suffer from depression;

[0054] The mice in the control group were raised in a free environment in the wild without restraint, and the other feeding conditions of the tw...

Embodiment 2

[0080] The construction method, device and application of the depression-induced breast cancer animal model provided in the embodiment of the present invention are basically the same as those in Example 1, except that the constructed spontaneous breast cancer transgenic mouse depression disorder animal Models for simulating human diagnosis of breast cancer and depression and related diseases. Specifically include the following steps:

[0081] 1. Rat tail genotype identification:

[0082] According to the instructions of the mouse tail genotype rapid identification kit (Beiyuntian, D7283M), a small amount of mouse tail was cut for identification. The mouse tail was submerged in the digestion solution, placed in a PCR instrument, set at 55°C for 15 minutes, 95°C for 5 minutes, added 100 μl StopSolution, and vortexed to mix. Perform PCR immediately after removal of undigested tissue.

[0083] The PCR reaction system is as follows:

[0084]

[0085] The PCR reaction paramet...

Embodiment 3

[0093] The construction method, device and application of the depression-induced breast cancer animal model provided in the embodiment of the present invention are basically the same as those in Examples 1 and 2, except that two MMTV-PyMT transgenic mouse models of depression were carried out comparison; the constructed animal model is applied to screen, test or verify the substance / therapeutic agent / treatment method that can alleviate or treat depression-related breast cancer. Specifically include the following steps:

[0094] 1. The rat tail genotype identification conditions are the same as in Example 2;

[0095] 2. (a) Model 1: The experimental conditions are the same as in Example 2. Hereinafter referred to as CRS.

[0096] (b) Model 2: Glucocorticoids were administered to mice at a dose of 0.18 mg / kg once a day for 3 weeks. Hereinafter referred to as CORT.

[0097] 3. Behavioral testing conditions are the same as in Example 1, see Figure 8 .

[0098] 4. The condit...

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Abstract

The invention discloses a construction method and device for a depression-induced breast cancer animal model and application. The construction method comprises the following steps that a mouse is placed in a constraining device not to act, the mouse is constrained for 4 hours every day, light-dark circulation is maintained for 12 hours for 3 weeks or more, and constructing is carried out to obtain the spontaneous breast cancer transgenic mouse depressive disorder animal model with depressive disorder related phenotypes. The invention further discloses a constraint device of the animal model. The animal model is used for simulating the human breast cancer and depression process and screening diagnosis and treatment drugs or therapies. The animal model has the advantages of good repeatability, small individual difference, short modeling time, low animal death rate and the like, and can be widely applied to simulation of human depression-induced breast cancer and related disease pathology and screening and curative effect verification of diagnosis and treatment substances.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method, device and application for constructing an animal model of breast cancer induced by depression. Background technique [0002] Depression (also known as depressive disorder) is a widespread chronic medical condition that can affect thought, mood, and physical health. It is characterized by persistently depressed mood, restlessness, and impaired motivation, and can range from psychomotor disturbances to cognitive impairments. Depression is also associated with serious physical health comorbidities, including cardiovascular disease, metabolic risk factors such as obesity, and premature death, with huge economic costs to society. Treatment and prevention of depression remain a public health priority. [0003] Breast cancer is the most common malignant tumor in women and one of the three most common cancers in the world. The probability of women suffering from breast cancer in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/027A01K15/04A01K1/06A01K1/03A61K49/00
CPCA01K67/0275A01K1/031A01K15/04A01K1/0613A61K49/0008A01K2217/00A01K2227/105A01K2267/0356
Inventor 陈前军张玉柱梁倩怡刘晓媛罗天裕李华超张宏艳卢海李东梅许锐任黎萍
Owner GUANGDONG HOSPITAL OF TRADITIONAL CHINESE MEDICINE
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