Major QTL for regulating and controlling brown rice rate of rice, molecular marker and application

A molecular marker, brown rice rate technology, applied in the fields of rice breeding and molecular biology, can solve the problems of limited research, and achieve the effect of accelerating breeding and high primer specificity

Active Publication Date: 2021-07-16
ZHEJIANG NORMAL UNIVERSITY
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004]At present, researchers have limited research on the fine mapping of rice brown rice rate QTL loci and related molecular markers; therefore, it is necessary to further dig and analyze rice brown rice rate QTL loci and related molecular markers provide new options for rice resistance breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Major QTL for regulating and controlling brown rice rate of rice, molecular marker and application
  • Major QTL for regulating and controlling brown rice rate of rice, molecular marker and application
  • Major QTL for regulating and controlling brown rice rate of rice, molecular marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1, the main effect QTL location of regulation rice brown rice rate

[0042] 1. Acquisition of experimental materials

[0043] Huazhan was used as the donor parent, and the rice variety Reyan No. 2 was used as the recipient parent for crossing, and the single-seed transmission method was used (that is, F1 was treated with bagging a single plant until the phenotype of the offspring lines did not segregate) , and finally got 120 stable genetic lines (F13, all lines are phenotypically stable), forming a recombinant inbred line RIL population, such as figure 1 .

[0044] Select 60 seeds of each parent and each strain (F13), soak the seeds for 2 days after surface disinfection, change the water every other day, wrap them in a moist towel, and place them in a 37°C incubator for 2 days to accelerate germination. In the wet state, choose the seeds with the same dew and white color for sowing. After 30 days, the parents with similar growth conditions and 24 seedling...

Embodiment 2

[0052] Example 2, molecular marker-assisted selection

[0053] Molecular markers Indel BRR-1 and molecular markers Indel BRR-2 were set on the upstream and downstream of the QTL site QBRR-1, respectively, and primers were designed;

[0054] The primer pair for molecular marker Indel BRR-1 is:

[0055] Upstream primer: 5'-TTCACCTTATTCCTCTACCCTCTT-3';

[0056] Downstream primer: 5'-GTATCCGAAAAACCCCTTCC-3';

[0057] The primer pair for molecular marker Indel BRR-2 is:

[0058] Upstream primer: 5'-GGCTGGATATTTTCCTTCCAT-3';

[0059] Downstream primer: 5'-CCACAGATGTGTGAGCAGAAA-3'.

[0060] Genomic DNA was extracted from the rice leaves of parents Reyan 2, Huazhan and its F1 generation and RIL population, and PCR amplification was performed on the genomic DNA using the above molecular markers;

[0061] PCR reaction system: 1 μL of upstream primer (concentration of 10 μM), 1 μL of downstream primer (concentration of 10 μM), 2 μL of DNA template (concentration greater than 50 ng / μ...

Embodiment 3

[0067] Example 3 Application of rice brown rice rate-related QTL in rice breeding

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the technical field of rice breeding and molecular biology, in particular to a major QTL for regulating and controlling the brown rice rate of rice, a molecular marker and application. The invention discloses a major QTL for regulating and controlling the brown rice rate of rice. The major QTL is located on a chromosome 10 of the rice and is named as QBRR-1, the genetic distance is 70.1-81.73 cM, and the physical distance is 17570591-19066686 bp. The invention also provides the molecular marker of the major QTL for regulating and controlling the brown rice rate of the rice. According to the major QTL, the rice with high brown rice rate is bred through the molecular marker.

Description

technical field [0001] The invention relates to the technical fields of rice breeding and molecular biology, and more specifically relates to a main effect QTL for regulating rice brown rice rate, a molecular marker and its application. Background technique [0002] Rice is one of the main food crops in the world. As one of the important qualities of rice, the processing quality has played an extremely important role in improving rice quality and increasing rice yield [1]. Now, due to the improvement of living standards, the quality of rice is getting better and better. Consumers pay more and more attention to cultivating rice with high processing quality plays an important role in agricultural development. The grinding quality of rice includes brown rice rate, polished rice rate and whole polished rice rate. The ratio of the mass of grains obtained from rice husking to the total mass of the sample is called the brown rice rate, and the ratio of the mass of brown rice groun...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 饶玉春芦涛王跃星林晗褚晓洁王盛叶涵斐陈芊羽方圆圆
Owner ZHEJIANG NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap