Polypeptide carrier for nucleic acid transfection and use thereof

A nucleic acid and carrier technology, which is applied in the preparation and application of polypeptide carriers, can solve the problems of imperfect preparation and application of polypeptide carriers, and difficulty in achieving the effect, so as to avoid toxicity and realize the effect of long-term circulation in the body

Inactive Publication Date: 2021-07-23
苏州合疗生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation and application of polypeptide vectors for nucleic acid transfection are not perfect at present, and it is difficult to achieve the expected effect

Method used

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  • Polypeptide carrier for nucleic acid transfection and use thereof

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Embodiment Construction

[0027] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0028] In the following description, many specific details are set forth in order to fully understand the present invention, but the present invention can also be implemented in other ways different from those described here, therefore, the protection scope of the present invention is not limited by the specific implementation disclosed below. Example limitations.

[0029] In the description of the present invention, it should be noted that unles...

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Abstract

The invention discloses a polypeptide carrier for nucleic acid transfection and use thereof. The polypeptide carrier comprises a shell and an inner core composed of polypeptide and nucleic acid. A surface of the polypeptide carrier is provided with negatively charged or electrically neutral biomacromolecular drug delivery nanoparticles with targeting groups; the shell is formed by sequentially and tightly covering and wrapping an irregular cationic lipid membrane and polyanions; the cationic lipid membrane is provided with a cationic double-layer phospholipid membrane, the polyanions are wrapped on surfaces of the nanoparticles through electrostatic and hydrogen-bond interaction, and the cationic lipid membrane is a cationic liposome or a cationic micelle formed by cationic lipid and neutral lipid or cholesterol or amphiphilic molecules; the polyanions are selected from hyaluronic acid or hyaluronic acid derivatives; and the exposed cationic lipid membrane breaks through an endosome membrane through a membrane melting effect, and polypeptide, nucleic acid and a compound of the polypeptide and the nucleic acid are released into cytoplasm, such that combined treatment of an antibody, the polypeptide and the nucleic acid for cancer with high expression of targeted HER2 is realized.

Description

technical field [0001] The invention relates to the field of preparation and application of polypeptide carriers, in particular to a polypeptide carrier for nucleic acid transfection and its application. Background technique [0002] Polypeptide is a compound formed by α-amino acids linked together by peptide bonds, and is an intermediate product of protein hydrolysis. The compound formed by the dehydration condensation of two amino acid molecules is called a dipeptide, and by analogy there are tripeptides, tetrapeptides, and pentapeptides. A compound usually formed by dehydration condensation of 10-100 amino acid molecules is called a polypeptide. Generally, the number of amino acids contained in a peptide is two to nine. According to the number of amino acids in the peptide, there are many different names for the peptide: the compound formed by the dehydration condensation of two amino acid molecules is called a dipeptide. Similarly, there are three Peptides, tetrapeptid...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/64A61K47/61A61K9/51A61K39/395A61P35/00A61K31/7088A61K38/02
CPCA61K9/5146A61K9/5161A61K31/7088A61K38/02A61K39/39558A61K47/61A61K47/645A61K47/6929A61K47/6939A61P35/00A61K2300/00
Inventor 钟天翼
Owner 苏州合疗生物技术有限公司
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