Preparation method of cobra venom nerve growth factor

Abstract

The invention discloses a preparation method of a cobra venom nerve growth factor. The method is characterized in that crude cobra venom is subjected to gel chromatography twice, wherein the gel chromatography I is SP-Sephadex G-25 column chromatography, and the gel chromatography II is Acta Rosorse column chromatography. The cobra venom nerve growth factor provided by the invention is simple and feasible in preparation method, can be used for industrial large-scale production, and is small in activity loss, and the activity loss of the cobra venom NGF is less than 25%; the conventional limitation of protein separation can be broken through, and the crude snake venom liquid with higher initial concentration can also be used for effective separation; the method is high in production efficiency, reasonable in cost and particularly suitable for industrial large-scale production; and the yield is high, exceeds 0.5%, and even can reach 0.6% or above.

Description

Technical field: [0001] The invention relates to a preparation method of cobra venom nerve growth factor, in particular to a method for separating and purifying the nerve growth factor from cobra venom by chromatography. Background technique [0002] Nerve Growth Factor (NGF), Nerve-like growth factor is a biologically active polypeptide that plays an important role in regulating the growth and development of various cells and neurons. Since the discovery of NGF in 1984 and the Nobel Prize in 1986, NGF has been found to maintain the growth of sympathetic and sensory nerve cells, promote the growth, development and metabolism of neurons, maintain the normal function of the brain and regulate the endocrine and immune systems, etc. It is an ideal drug for the treatment of brain injury, senile dementia, meningitis, angioneurotic headache, Parkinson's syndrome and other diseases related to nerve damage and neurodegeneration. [0003] Nerve injury has always been considered as an...

AI Technical Summary

Problems solved by technology

But this method has these following two fatal weaknesses: (1) generally all need more than 100 hours in the gel filtration process of the first step, not only operation time is long, and the long-time contact of albumen and medium makes required The activity of the target protein may be reduced

(2) The gel filtration method used in the first step can only be used for the preparation of a small amount of samples in the laboratory, and it is difficult to scale up to medium pilot and production scale

[0005] It can be said that despite a lot of research in the laboratory, there is still no industrial-grade, high-yield, low-activity loss, and high-efficiency preparation method for snake venom NGF, especially no preparation method for snake venom NGF that can be used for injections.

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