Method for increasing proliferation coefficient by increasing and jointing pseudobulb of cremastra appendiculata

Abstract

The invention discloses a method for increasing proliferation coefficient by increasing and jointing pseudobulb of cremastra appendiculata. The method comprises the following steps: firstly, inoculating protocorm of the cremastra appendiculata into a cluster bud induction culture medium for culturing, obtaining cluster buds after 30 days, adding a sterilized pseudobulb inducer and joint increasing and jointing inducer, and forming clustered pseudobulb from the cluster buds after 60 days, wherein the number of the pseudobulb sections is 2-6, and the distance between the joints is long; and cutting the clustered pseudobulb of the cremastra appendiculata into single pseudobulb, transversely cutting each pseudobulb into a stem section with one stem node, and respectively inoculating the stem sections into a proliferation culture medium to achieve the purpose of improving the proliferation coefficient of cremastra appendiculata. According to the method, the pseudobulb and the joint increasing and jointing inducer thereof are added to the culture medium of the cremastra appendiculata cluster buds, so that the number of joints on the pseudobulb is increased, the distance between the joints is increased, and the multiplication coefficient can be increased by 2.7-4.1 times on the original basis and can reach 21.32 to the maximum. The method is good in repeatability, safe, efficient, energy-saving and consumption-reducing, and provides technical support for applying the tissue culture and rapid propagation technology of cremastra appendiculata to industrialized production.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for increasing the multiplication coefficient by increasing the joints of rhododendron orchid pseudobulbs. Background technique [0002] Rhododendron ( Cremastraappendiculata (D. Don) Makino) and the dried pseudobulb of Suanlan are Chinese medicine Arrowhead, which has been recorded in the "Pharmacopoeia of the People's Republic of China" over the years. , scrofula, sputum nucleus, lymph node tuberculosis, snake bites, lame lumps and lumps. In addition, Rhododendron Orchid has anti-tumor, antibacterial, blood fat-lowering, blood-sugar-lowering effects, and has attracted widespread attention at home and abroad. [0003] Rhododendron orchid is a perennial rare medicinal orchid. It grows wild in forest wetlands or ditchside wetlands at an altitude of 500-2900 meters. It is mainly produced in Sichuan, Yunnan, Guizhou, Guangxi and other places, in southern Gansu, Shaanxi and o...

AI Technical Summary

Problems solved by technology

The wild breeding of Rhododendron orchid is slow, mainly due to the following two reasons: on the one hand, because the seeds of Rhododendron orchid have no endosperm, the embryo is only a group of undifferentiated mature cells, which is extremely difficult to germinate under natural conditions; on the other hand, Rhododendron orchid The pseudobulb is its only reproductive organ. Every year, only one bud germinates from its annual pseudobulb and grows into a new pseudobulb. After many years of growth, it forms a pseudobulb string with low reproduction coefficient and extremely low yield. Basically, the whole plant is dug up, and the wild Rhododendron orchid is dug up with one less plant, resulting in a sharp decrease in wild resources.