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Clostridium difficile specific antigen peptide

A Clostridium difficile, specific technology, applied in the direction of bacterial antigen components, specific peptides, peptides, etc., can solve problems such as the inability to effectively eliminate Clostridium difficile

Active Publication Date: 2021-11-30
SHIJIAZHUANG HIPRO BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current results of immunoassays on these surface-associated proteins show that these proteins still cannot effectively eliminate Clostridium difficile, and there is an urgent need to find more immunogenic target proteins for the preparation of Clostridium difficile vaccines, in order to prepare specific and effective vaccines. Clostridium difficile vaccine

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  • Clostridium difficile specific antigen peptide

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Embodiment Construction

[0017] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0018] 1. Cultivation of Clostridium difficile

[0019] The present invention selects 4 high-toxin-producing Clostridium difficile RT027 strains (RT027 / NAP1 / BI / ST01) and 3 low-toxin-producing Clostridium difficile RT017 strains for bacterial culture. Before the strains are used, thaw the frozen-preserved strains at room temperature, inoculate them on CDMN selective medium, and place them in an anaerobic tank at 37°C for 48-72 hours after marking three sections. Pick a single colony and transfer it to the CDMN culture medium for iso...

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Abstract

The invention relates to a clostridium difficile specific antigen peptide, in particular to a specific antigen peptide for a high-poison-yield clostridium difficile RT027. The clostridium difficile specific antigen peptide comprises an amino acid sequence as shown in SEQ ID No.1. A specific peptide fragment and a specific antibody thereof are used, a novel clostridium difficile high-poison-yield strain detection kit and a clostridium difficile vaccine can be developed, and the clostridium difficile specific antigen peptide has an important significance on detection and treatment of clostridium difficile high-poison-yield strain infection.

Description

technical field [0001] The invention relates to a Clostridium difficile specific antigen peptide. Background technique [0002] Clostridium difficile ( Clostridioides difficile , CD) is a Gram-positive obligate anaerobic spore-forming bacterium. CD is the main pathogenic bacterium of antibiotic-associated diarrhea (AAD) in hospitals, which can cause mild to severe diarrhea, colitis, pseudomembranous colitis, toxic megacolon, etc., and even death in severe cases. In recent years, the emergence of highly toxin-producing Clostridium difficile (RT027 / NAP1 / BI / ST01) has gradually increased the morbidity and mortality of Clostridiodes difficile infection (CDI), causing tens of thousands of deaths worldwide every year. The cost ran into the billions of dollars. [0003] In 2013, the US CDC (Center for Disease Control and Prevention) listed Clostridium difficile infection as the first urgent level of "microorganisms causing public health threats". Clostridium difficile is mainly ...

Claims

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Application Information

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IPC IPC(8): C07K14/33C12N15/31C07K16/12A61K39/08A61P31/04
CPCC07K14/33C07K16/1282A61K39/08A61P31/04Y02A50/30
Inventor 赵建宏李志荣张慧敏
Owner SHIJIAZHUANG HIPRO BIOTECH
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