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Protein fragment complementary system based on split luciferase Akaluc and construction method thereof

A protein fragment, luciferase technology, applied in the fields of botanical equipment and methods, biochemical equipment and methods, enzymes, etc., can solve problems that have not yet been developed

Active Publication Date: 2021-11-30
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a protein complementation system based on the luciferase Akaluc has not been developed for the study of protein-protein interactions

Method used

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  • Protein fragment complementary system based on split luciferase Akaluc and construction method thereof
  • Protein fragment complementary system based on split luciferase Akaluc and construction method thereof
  • Protein fragment complementary system based on split luciferase Akaluc and construction method thereof

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Embodiment Construction

[0025] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. It should be understood that the specific embodiments described here are only used to explain the present application, but not to limit the present application. In addition, it should be noted that, for the convenience of description, only some structures related to the present application are shown in the drawings but not all structures. Based on the embodiments in this application, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of this application.

[0026] The terms "first", "second", etc. in this application are used to distinguish different objects, not to describe a specific order. Furthermore, the terms "include" and "have", as well as any variations thereof, are intended to cover a non-e...

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Abstract

The invention relates to the technical field of protein interaction imaging, and provides a protein fragment complementary system based on split luciferase Akaluc and a construction method thereof. The protein fragment complementary system comprises a first vector and a second vector, the first vector is a vector containing a sequence SEQ ID NO.2, and the second vector is a vector containing a sequence SEQ ID NO.3. The protein fragment complementary system is a complementary system based on the split luciferase Akaluc, the luciferase Akaluc can react with a substrate Akalumine, and fluorescence is generated under the condition of physiological temperature (37 DEG C). Furthermore, the fluorescence generated by the reaction of the luciferase Akaluc and the substrate Akalumine has longer wavelength, so that the fluorescence generated by the protein fragment complementary system has better tissue permeability and is beneficial to imaging observation.

Description

technical field [0001] This application relates to the technical field of protein interaction imaging, in particular to a protein fragment complementation system based on split luciferase Akaluc and a construction method of the protein fragment complementation system based on split luciferase. Background technique [0002] The interaction between proteins plays an important role in the life process of organisms. For example, many protein interactions are involved in gene regulation, cell signal transduction, and tumor growth and development. Monitoring these protein-protein interactions is particularly important for the dissection of life processes. In the past few decades, some methods based on fluorescence imaging have been developed and used to study the interaction between proteins, such as: fluorescence resonance energy transfer (fluorescence resonance energy transfer, FRET), based on singlet oxygen triplet energy transfer Imaging technology and bimolecular fluorescen...

Claims

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Application Information

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IPC IPC(8): C12N15/63C12N15/53C12Q1/66
CPCC12N15/63C12N9/0069C12Q1/66
Inventor 陈明海张先恩严闯
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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