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Cultivation method for improving corn haploid doubling efficiency

A cultivation method and haploid technology, which are applied in the field of cultivation to improve the doubling efficiency of corn haploids, can solve the problems affecting the doubling efficiency and scale, the reduced survival rate of seedlings, and insufficient light time, so as to save labor costs and have good ventilation , the effect of simple operation

Pending Publication Date: 2021-12-10
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional hardening and slowing down of seedlings and transplanting are carried out with sunshade nets, and the sprayed solution has single nutrients, insufficient light time, low growth temperature and mechanical damage to seedlings, etc., resulting in a greatly reduced survival rate of seedlings
Affect doubling efficiency and scale, reduce breeding efficiency

Method used

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  • Cultivation method for improving corn haploid doubling efficiency
  • Cultivation method for improving corn haploid doubling efficiency
  • Cultivation method for improving corn haploid doubling efficiency

Examples

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Embodiment 1

[0045] A cultivation method for improving the doubling efficiency of corn haploid, the specific steps are as follows:

[0046] (1) Build a plastic greenhouse: the shape of the greenhouse is built in a bow shape, and the height inside the shed is 1.8-2.0m. "Special vegetable greenhouse insect-proof net" is built to keep the ventilation and insect-proof in the shed, and the temperature is kept at 28-32 ℃; see figure 1 ;

[0047] (2) Preparation of special nutrient soil: Mix Danish pinch peat soil, perlite and peat soil in a ratio of 5:1:4 to obtain special nutrient soil for use; see the preparation and filling of special nutrient soil figure 2 ;

[0048] (3) Prepare nutrient solution and disinfectant: Dilute the "Hua Wuque" brand nutrient fertilizer at 1:500, dilute the rooting powder at 1:200, and mix the diluted nutrient fertilizer and diluted rooting powder in equal volumes Make a mixed special nutrient solution for use; dilute fenzamid at a ratio of 1:1000 and stir evenl...

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Abstract

The invention discloses a cultivation method for improving the corn haploid doubling efficiency, and belongs to the technical field of crop cultivation. According to the cultivation method for improving the corn haploid doubling efficiency, special nutrient soil is adopted, a nutrient solution is added, corn haploid seedlings are transplanted into kraft paper bags and then directly enter a greenhouse to be transplanted, hardened and recovered, the temperature and humidity in the greenhouse are kept, the seedlings are hardened, the special nutrient solution and disinfectant are sprayed, the soil in the kraft paper bags is kept moist, and careful field management is conducted. According to the method, the outdoor seedling hardening and recovering process is omitted, mechanical damage to the seedlings during transplanting in the greenhouse is reduced, the survival rate of the haploid seedlings can reach about 80%-90%, the corn haploid doubling rate can be increased by about 25%-34%, the breeding material and labor cost is reduced, environmental pollution is reduced, the haploid breeding efficiency is improved, operation is easy and convenient, and large-scale popularization and application can be realized.

Description

technical field [0001] The invention relates to the technical field of crop cultivation, in particular to a cultivation method for improving the doubling efficiency of corn haploids. Background technique [0002] In recent years, the maize haploid breeding technology based on biological induction has gradually become the key technology of maize breeding. Doubled haploid (DH) technology is the fastest, most convenient, economical, practical and direct method for breeding maize inbred lines. Its basic process is to obtain homozygous diploid plants through artificial or natural doubling of biologically induced haploid plants, and then select superior lines from them. Its main feature is that the line selection is fast, and a stable pure line can be obtained in only 2 generations (traditional inbred line selection requires more than 7 generations). [0003] With the breeding of new induction lines (Nongda Gaoyu No. 1, Jigaoyu No. 3, and Nongda Gaoyu No. 5), the efficiency of i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/20A01G24/28A01G24/15A01G31/00A01G9/14A01G13/10A01G31/02
CPCA01G22/20A01G24/28A01G24/15A01G31/00A01G9/14A01G13/10A01G31/02Y02A40/25Y02P60/21
Inventor 刘文国孟令聪何欢周德龙张建新郑淑波高嵩岳尧海路明周旭东王敏马英杰张志军邢跃先卢实李穆王楠高婷婷王薪淇
Owner JILIN ACAD OF AGRI SCI
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