Active intracellular Raman spectrum detection method

Abstract

The invention provides an active intracellular Raman spectrum detection method. The active intracellular Raman spectrum detection method comprises the following steps: providing a thermal gradient generation layer, and arranging cell sap on the thermal gradient generation layer; injecting particles capable of exciting surface plasmons into the cell sap; injecting a surfactant into the cell sap; after the surfactant is injected into the cell sap, introducing the particles into cells in the cell sap by adopting optical tweezers; after the particles are guided into the cells, controlling the particles to move to a designated area in the cells through optical tweezers; and after the particles move to the designated area, performing Raman spectrum detection on the cells. According to the Raman spectrum detection method, the particles capable of exciting the surface plasmons are induced to enter the cells, the surface plasmons generated by the exciting particles enhance Raman signals of molecules in the cells, particle enhanced Raman scattering in the cells is realized, and the detection intensity is improved.

Description

technical field [0001] The invention relates to the technical field of Raman signal sample detection, in particular to an active intracellular Raman spectrum detection method. Background technique [0002] Cells are the basic structural and functional units of organisms, composed of complex biomolecules wrapped in membranes, and these constituent molecules are distributed in different positions within the cell to achieve their respective functions. The precise detection and identification of these elements is an important issue in life science research. At present, methods for studying cell phenotypes at home and abroad include surface-enhanced Raman scattering, but the detection intensity of traditional Raman signals is too weak and the signal acquisition time is too long, which greatly affects limit its practical application. Contents of the invention [0003] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the detec...

AI Technical Summary

Problems solved by technology

The precise detection and identification of these elements is an important issue in life science research. At present, the methods for studying cell phenotypes at home and abroad include surface-enhanced Raman scattering, but the traditional Raman signal detection intensity is too weak and the signal acquisition time is too long, which greatly affects limit its practical application

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