30results about How to "Enhanced Raman scattering signal" patented technology

The invention discloses preparation and application of an SERS-fluorescence dual-mode optical imaging probe with a tumor double-targeting function. The nano-probe is composed of an imaging function core part and a targeting function shell part. The imaging function core part comprises silver nanoparticles adsorbed with Raman reporter molecules and a silicon dioxide shell layer doped with fluorescence dye, so that the probe has the SERS imaging function and the fluorescence imaging function simultaneously. The targeting function shell comprises a polyethylene glycol (PEG) coupling agent and two polypeptides specifically targeting tumor cells. According to the probe, the more effective targeting capacity is supplied to the nanoparticles by adopting a dual-polypeptide modification method, therefore, the probe can well enter the tumor cells, recognition of the probe to the tumor cells is enhanced, and SERS-fluorescence dual-mode imaging on a tumor is achieved.

The invention provides a Cu2O-Au composite microstructure surface enhanced Raman scattering active substrate and a production method thereof. The method comprises the following steps: chelating citrate ions and copper ions, shaping with polyvinylpyrrolidone, and carrying out a reaction under water bath conditions with glucose as a reducing agent to generate Cu2O octahedral crystals; and dispersing the Cu2O octahedral crystals in water, adding a AuCl<4><-> solution, reducing AuCl<4><-> by the Cu2O octahedral crystals to form Au nanoparticles, and carrying out Au nanoparticle in situ deposition on the surface of octahedral Cu2O to reduce the surface energy of a system and generate Cu2O-Au composite microparticles. A Cu2O-Au composite microstructure is designed and synthesized to develop the surface enhanced Raman scattering activity of a semiconductor Cu2O, and local surface plasma resonance of Au aggregate and strong electromagnetic field generated in an interface due to charge transfer between Cu2O and Au are used to improve the surface enhanced Raman scattering activity of the Cu2O-Au composite microstructure.

The invention provides a measurement method of body fluid drug concentration based on a surface enhanced Raman spectrum. The method comprises the following steps: obtaining body fluid containing a drug from a patient, obtaining a purified sample by a conventional pretreatment method, placing a certain amount of the sample in a container containing a nano-gold or nano-silver solution with standardconcentration, uniformly mixing the sample with the nano-gold or nano-silver solution by shaking or swinging or rotating the container, then forming a uniform thin layer with a certain thickness at the bottom of the container through centrifugal concentration, directly performing transmission Raman spectrum detection on the uniform thin layer formed at the bottom of the container to obtain a surface enhanced Raman spectrogram of drug molecules, and obtaining a drug content in the blood via model calculation. The invention further provides a device for implementing the above method. By means ofthe detection and analysis of the surface enhanced transmission Raman spectrum, the drug concentration measurement is performed, and the measured minimum concentration limit reaches a ppb level.

The invention relates to a local electromagnetic field enhancement device for Raman spectrum characterization as well as a preparation method, application and a utilization method thereof and belongsto the technical field of detection. The device comprises a composite metal nano-pore array substrate and an atomic force microscope probe, wherein the composite metal nano-pore array substrate is sequentially composed of a hard substrate, a chrome layer, a gold layer and a silver layer with the upper surface coated with a gold film from bottom to top; nano-pore arrays are arranged on the silver layer and the gold layer; the vertical depth of nano-pores is less than the sum of the thicknesses of the silver layer and the gold layer; pore walls of the nano-pores are coated with gold films; the surface of the atomic force microscope probe is coated with a silver film; the surface of a probe tip of the probe is provided with stripes. By adopting the device provided by the invention, single-molecule-grade molecular fingerprint spectrum characterization analysis can be realized, so that the disadvantage that configuration and conformation difference of a real single molecule on the surface of the substrate cannot be obtained by common Raman or surface enhanced scattering Raman is overcome; the local electromagnetic field enhancement device also can be applied to characterization of configuration and conformation of isomer molecules and discriminatory analysis of isomers in a single molecule level is realized.

The invention discloses a preparation method of nanogold, and belongs to the technical field of inorganic nano material preparation. The preparation method of the nanogold comprises the following steps of 1, preparing gold seeds; 2, preparing a nanogold ball colloidal solution; 3, preparing an Au@PbS core-shell heterogeneous nano material aqueous solution; 4, preparing an Au/Pbs/Au nano structuresolution; and 5, preparing the nanogold. The nanogold prepared through the method has excellent performance of a stable structure, an adjustable nanogold shell layer size, an obvious plasmon couplingeffect, a strong surface enhancement Raman scattering signal and the like, and has important reference significance for the construction of a surface Raman enhancer.

The invention discloses an electron-doped ZnO nanocrystalline substrate and a preparation method and application. The preparation method comprises the following steps that 1, electron-doped ZnO nanocrystalline is prepared by adopting a hot injection method; 2, surface treatment is conducted on the electron-doped ZnO nanocrystalline by adopting Mills salt to remove long carbon chain ligands on thesurface, then the surface is modified with C2-C8 carbon chain ligands, and the modified electron-doped ZnO nanocrystalline is obtained; and 3, the modified electron-doped ZnO nanocrystalline is prepared into a solution, the solution is applied to a substrate and dried, and the electron-doped ZnO nanocrystalline substrate is obtained. According to the electron-doped ZnO nanocrystalline substrate and the preparation method and application, surface treatment is conducted on the electron-doped ZnO nanocrystalline through the Mills salt, the surface is modified with the C2-C8 carbon chain ligands,therefore, the dispersity of the nanocrystalline can be kept, the film forming capability of the nanocrystalline on the substrate is improved, and Raman scattering signals and infrared absorption signals of probe molecules are enhanced.

The invention belongs to the technical field of nanometre, and discloses a preparation method of a triangular silver nano-sheet. The triangular silver nano-sheet prepared by the method is high in purity, and has stability in dark storage for at least 30 days in environmental conditions, wherein the stability is detected according to the fact that displacement of the absorption peak wavelength of an ultraviolet visible light spectrum is no more than 10 nm; the triangular silver nano-sheet has good thermal stability, and the heat resistance temperature is as high as 95 DEG C; the triangular silver nano-sheet has high photothermal conversion efficiency of 42%; the triangular silver nano-sheet has good biocompatibility, and a material of the triangular silver nano-sheet is neutral, does not change the pH environment of a biological system, and is low in biotoxicity; and stable surface-enhanced Raman scattering signals can be generated under near-infrared light irradiation.

The invention discloses a fluorescence and Raman detection device based on a lens group, and relates to the field of Raman detection. The device comprises a laser source, a lens group and a volume Bragg grating which are arranged in sequence, wherein the laser source is used for emitting laser to the lens group, the lens group is used for focusing the laser, the volume Bragg grating and the lasersource form a Fabry-Perot cavity, and a sample to be detected is arranged at a focus point of the lens group and used for generating Raman scattering according to the focused laser. According to the fluorescence and Raman detection device based on the lens group provided by the invention, the laser light source with relatively small energy is used, the Fabry-Perot gain cavity is formed between thevolume Bragg grating and the laser source to enhance laser, and the laser is focused through the lens group, so that the sample to be detected is enabled to be located at a focal point in the middleof the lens group, the laser emitted to the sample is enhanced, and a Raman scattering signal generated by the sample is also enhanced, thereby facilitating discrimination.

The invention provides an active intracellular Raman spectrum detection method. The active intracellular Raman spectrum detection method comprises the following steps: providing a thermal gradient generation layer, and arranging cell sap on the thermal gradient generation layer; injecting particles capable of exciting surface plasmons into the cell sap; injecting a surfactant into the cell sap; after the surfactant is injected into the cell sap, introducing the particles into cells in the cell sap by adopting optical tweezers; after the particles are guided into the cells, controlling the particles to move to a designated area in the cells through optical tweezers; and after the particles move to the designated area, performing Raman spectrum detection on the cells. According to the Raman spectrum detection method, the particles capable of exciting the surface plasmons are induced to enter the cells, the surface plasmons generated by the exciting particles enhance Raman signals of molecules in the cells, particle enhanced Raman scattering in the cells is realized, and the detection intensity is improved.