30results about How to "Enhanced Raman scattering signal" patented technology

The invention discloses preparation and application of an SERS-fluorescence dual-mode optical imaging probe with a tumor double-targeting function. The nano-probe is composed of an imaging function core part and a targeting function shell part. The imaging function core part comprises silver nanoparticles adsorbed with Raman reporter molecules and a silicon dioxide shell layer doped with fluorescence dye, so that the probe has the SERS imaging function and the fluorescence imaging function simultaneously. The targeting function shell comprises a polyethylene glycol (PEG) coupling agent and two polypeptides specifically targeting tumor cells. According to the probe, the more effective targeting capacity is supplied to the nanoparticles by adopting a dual-polypeptide modification method, therefore, the probe can well enter the tumor cells, recognition of the probe to the tumor cells is enhanced, and SERS-fluorescence dual-mode imaging on a tumor is achieved.

The invention relates to a trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer, and belongs to the field of pesticide detection. The invention uses Raman active dye labeled gold nanoparticles as substrates, and uses the specific binding of pesticide and the aptamer binding to cause aggregation difference of reactive dye labeled gold nanoparticles in salt solution; a corresponding reactive dye SERS spectrum is obtained by a portable Raman spectrometer, so as to realize the quantitative detection of trace pesticide; and feasibility testing of the method is carried out in an actual tea sample. The method provided by the invention converts a conventional thinking of using the Raman characteristic peak of a target object to be detected as quantification determination in the traditional Raman technology into using the Raman characteristic peak of the reactive dye marker as the quantitative standard for target detection, so as to not only improve the detection sensitivity, simplify the preparation process of complex substrate, and provide convenience for research, inspection and supervision of trace pesticide residues in the fields of food industry.

The inventing discloses a surface enhanced Raman scattering sensor. The surface enhanced Raman scattering sensor comprises a substrate and a metal nanometer pillar array arranged on the substrate, wherein the upper end surface of a metal nanometer pillar is planar, and the cross section of the metal nanometer pillar is hexagonal; the height of the metal nanometer pillar is 400-600nm and the diameter of the metal nanometer pillar is 100-200nm; and a distance between two adjacent metal nanometer pillars is 200-500nm. The invention also provides a preparation method of the surface enhanced Raman scattering sensor. According to the surface enhanced Raman scattering sensor, an anodic aluminum oxide template is used for assisting to obtain an array type nanometer pillar structure, a Raman scattering signal can be remarkably enhanced, and the surface enhanced Raman scattering sensor can be applied to the fields such as active biomacromolecules, narcotics, explosives, food sanitation, medical imaging, environment detection and the like; and besides, the preparation process of the sensor is simple, the conductive aluminum membrane layer is prepared by the printing process, the raw materials are saved, the production cost is reduced, the sensor is green and environmentally friendly, and the sensor is suitable for mass production.

The invention provides a Cu2O-Au composite microstructure surface enhanced Raman scattering active substrate and a production method thereof. The method comprises the following steps: chelating citrate ions and copper ions, shaping with polyvinylpyrrolidone, and carrying out a reaction under water bath conditions with glucose as a reducing agent to generate Cu2O octahedral crystals; and dispersing the Cu2O octahedral crystals in water, adding a AuCl<4><-> solution, reducing AuCl<4><-> by the Cu2O octahedral crystals to form Au nanoparticles, and carrying out Au nanoparticle in situ deposition on the surface of octahedral Cu2O to reduce the surface energy of a system and generate Cu2O-Au composite microparticles. A Cu2O-Au composite microstructure is designed and synthesized to develop the surface enhanced Raman scattering activity of a semiconductor Cu2O, and local surface plasma resonance of Au aggregate and strong electromagnetic field generated in an interface due to charge transfer between Cu2O and Au are used to improve the surface enhanced Raman scattering activity of the Cu2O-Au composite microstructure.

The invention provides a measurement method of body fluid drug concentration based on a surface enhanced Raman spectrum. The method comprises the following steps: obtaining body fluid containing a drug from a patient, obtaining a purified sample by a conventional pretreatment method, placing a certain amount of the sample in a container containing a nano-gold or nano-silver solution with standardconcentration, uniformly mixing the sample with the nano-gold or nano-silver solution by shaking or swinging or rotating the container, then forming a uniform thin layer with a certain thickness at the bottom of the container through centrifugal concentration, directly performing transmission Raman spectrum detection on the uniform thin layer formed at the bottom of the container to obtain a surface enhanced Raman spectrogram of drug molecules, and obtaining a drug content in the blood via model calculation. The invention further provides a device for implementing the above method. By means ofthe detection and analysis of the surface enhanced transmission Raman spectrum, the drug concentration measurement is performed, and the measured minimum concentration limit reaches a ppb level.

The invention provides a surface enhanced Raman substrate and a preparation method thereof and relates to the technical field of material structure detection. The surface enhanced Raman substrate is used for detecting a liquid sample; the surface enhanced Raman substrate comprises a substrate, a gold film and gold nano-cubes; the substrate is made of a silicon material; the gold film is arranged on the substrate and the gold nano-cubes are arranged on the gold film; the gold nano-cubes are arrayed in a matrix; gaps are formed between the gold nano-cubes; the gaps are used for injecting the liquid sample to be detected. The gold nano-cubes are arrayed in the matrix and the formed gaps are regularly arrayed and have a uniform size; after the product to be detected is injected to the gaps, the product to be detected has good uniformity and an excited solution Raman scattering signal is enhanced; the surface enhanced Raman substrate has high sensitivity and good repeatability and structureinformation of a molecular level of the product to be detected can be relatively accurately and clearly detected.

The invention relates to enhanced Raman scattering substrates of silicon semiconductor which possessed good biological compatibility, a preparation method for the same, and the detection of rhodamine 6G molecules and 4-aminothiophenol molecules in solution with the said substrate. The invention adopts a chemical etching method to etch vertically-arranged silicon nanowire array in the surface of the monocrystalline silicon chip; then flocculent silver branches produced as by-products in the silicon nanowire array top in the etching process are removed; and a lot of Si-H bond are decorated in the nanowire surface. The enhanced Raman scattering substrates of silicon semiconductor are constituted by the vertically-arranged silicon nanowire array in the surface of the monocrystalline silicon chip and do not contain precious metal silver; the silicon nanowire surface is decorated with Si-H bonds. The invention realizes for the first time the preparation of enhanced Raman scattering substrates of silicon emiconductor which possessed good biological compatibility with only silicon materials rather than any precious metals.

The invention relates to a local electromagnetic field enhancement device for Raman spectrum characterization as well as a preparation method, application and a utilization method thereof and belongsto the technical field of detection. The device comprises a composite metal nano-pore array substrate and an atomic force microscope probe, wherein the composite metal nano-pore array substrate is sequentially composed of a hard substrate, a chrome layer, a gold layer and a silver layer with the upper surface coated with a gold film from bottom to top; nano-pore arrays are arranged on the silver layer and the gold layer; the vertical depth of nano-pores is less than the sum of the thicknesses of the silver layer and the gold layer; pore walls of the nano-pores are coated with gold films; the surface of the atomic force microscope probe is coated with a silver film; the surface of a probe tip of the probe is provided with stripes. By adopting the device provided by the invention, single-molecule-grade molecular fingerprint spectrum characterization analysis can be realized, so that the disadvantage that configuration and conformation difference of a real single molecule on the surface of the substrate cannot be obtained by common Raman or surface enhanced scattering Raman is overcome; the local electromagnetic field enhancement device also can be applied to characterization of configuration and conformation of isomer molecules and discriminatory analysis of isomers in a single molecule level is realized.

The embodiment of the invention discloses a method for surface enhanced Raman scattering detection. The method comprises the following step: preparing core-shell structures of silver core silicon dioxide shells deposited in a nano pore array on an AAO template. When the silver core silicon dioxide shells are taken as a substrate for surface enhanced Raman spectra, the core-shell structures form octahedral interstices, tetrahedral interstices or other irregular interstices like crystal structures in the stacking process. The interstices can form attachment points of probe molecules. As pore wall of the nano pore array on the AAO template is transparent and the shells of the core-shell structures are transparent, incident Raman detection laser can be directly focused to inner cores of the core-shell structures near the probe molecules to enhance the Raman scattering signals of the probe molecules, so that efficient surface enhanced Raman scattering signals are acquired.

The invention discloses a preparation method of nanogold, and belongs to the technical field of inorganic nano material preparation. The preparation method of the nanogold comprises the following steps of 1, preparing gold seeds; 2, preparing a nanogold ball colloidal solution; 3, preparing an Au@PbS core-shell heterogeneous nano material aqueous solution; 4, preparing an Au / Pbs / Au nano structuresolution; and 5, preparing the nanogold. The nanogold prepared through the method has excellent performance of a stable structure, an adjustable nanogold shell layer size, an obvious plasmon couplingeffect, a strong surface enhancement Raman scattering signal and the like, and has important reference significance for the construction of a surface Raman enhancer.

The invention discloses an electron-doped ZnO nanocrystalline substrate and a preparation method and application. The preparation method comprises the following steps that 1, electron-doped ZnO nanocrystalline is prepared by adopting a hot injection method; 2, surface treatment is conducted on the electron-doped ZnO nanocrystalline by adopting Mills salt to remove long carbon chain ligands on thesurface, then the surface is modified with C2-C8 carbon chain ligands, and the modified electron-doped ZnO nanocrystalline is obtained; and 3, the modified electron-doped ZnO nanocrystalline is prepared into a solution, the solution is applied to a substrate and dried, and the electron-doped ZnO nanocrystalline substrate is obtained. According to the electron-doped ZnO nanocrystalline substrate and the preparation method and application, surface treatment is conducted on the electron-doped ZnO nanocrystalline through the Mills salt, the surface is modified with the C2-C8 carbon chain ligands,therefore, the dispersity of the nanocrystalline can be kept, the film forming capability of the nanocrystalline on the substrate is improved, and Raman scattering signals and infrared absorption signals of probe molecules are enhanced.

The invention discloses a micro detection device, and a detection method. The micro detection device comprises an excitation light source, a detection probe, a Raman spectrum analyzer, and an opticalfiber circulator which is capable of realizing single way transmission of light along the excitation light source, the detection probe, and the Raman spectrum analyzer; a vacuum chamber, a hollow photonic crystal optical fiber communicated with the vacuum chamber, and a gas pump are arranged in the detection probe; a nanometer structure metal layer is arranged on a cavity internal wall of the hollow photonic crystal optical fiber. The beneficial effects are that: the hollow photonic crystal optical fiber and surface enhanced Raman scattering technology are combined, Raman scattering signals are enhanced greatly, and at the same time, optical fiber is taken as a light transmission medium, an optical fiber device is taken as a light splitting element. The micro detection device is of a all optical fiber structure, is small and portable, is suitable for a plurality of situations, is extremely high in detection sensitivity, is suitable for trace quantity detection and high concentration detection, and is extremely high in efficiency.

The invention relates to a bi-conical tapered fiber evanescent wave coupling-based fiber Raman sensor detection device, which comprises a monochromatic source and a high-sensitivity Raman spectrometer, wherein the monochromatic source sequentially passes through a polaroid sheet, a focusing lens, a fiber coupling platform and a fused biconical taper fiber to be connected to the high-sensitivity Raman spectrometer, and the fused biconical taper fiber is placed in a solution to be detected; and in a taper zone of the fused biconical taper fiber, evanescent waves excite the molecules, absorbed bya metal nanoparticle layer on the surface of the fiber, of the solution to be detected to enable the molecules of the solution to be detected to generate a Raman spectrum which is coupled back to thefiber through the taper zone of the fiber and transmitted to the high-sensitivity Raman spectrometer to detect the Raman spectrum of the solution to be detected. The device is simple in structure, high in anti-interference capacity, high in flexibility and is suitable to be used on various occasions such as online analysis, real-time detection and biopsy specimen analysis.

The present invention relates to a surface-enhanced raman scattering torquemaster based on an optical fiber fuse-tapered coupler, comprising two monochromatic light sources, a 2*2 fuse-tapered optical fiber coupler and two high-sensitivity raman spectrometers. The two monochromatic light sources are connected to the two high-sensitivity raman spectrometers sequentially through two polaroid sheets, two focusing lens, two optical fiber coupling platforms and the 2*2 fuse-tapered optical fiber coupler; the 2*2 fuse-tapered optical fiber coupler is positioned in the solution or the gas to be tested; on the fused tapering zone part of the 2*2 fuse-tapered optical fiber coupler, evanescent wave excites the solution or gas molecule to be tested which is absorbed by a optical fiber surface metal nanometer particles layer, so as to generate raman spectrum, couple into the optical fiber through the optical fiber fused tapering zone, and transmit to the high-sensitivity raman spectrometers, to detect the raman spectrum of the solution or the gas molecule to be tested. The device has simple structure, strong anti-interference capacity and high sensitivity, and is applicable to various fields such as on-line analysis, real-time detection, biopsy sample analysis and the like.

The invention belongs to the technical field of nanometre, and discloses a preparation method of a triangular silver nano-sheet. The triangular silver nano-sheet prepared by the method is high in purity, and has stability in dark storage for at least 30 days in environmental conditions, wherein the stability is detected according to the fact that displacement of the absorption peak wavelength of an ultraviolet visible light spectrum is no more than 10 nm; the triangular silver nano-sheet has good thermal stability, and the heat resistance temperature is as high as 95 DEG C; the triangular silver nano-sheet has high photothermal conversion efficiency of 42%; the triangular silver nano-sheet has good biocompatibility, and a material of the triangular silver nano-sheet is neutral, does not change the pH environment of a biological system, and is low in biotoxicity; and stable surface-enhanced Raman scattering signals can be generated under near-infrared light irradiation.

The invention discloses a gold-based extraction material functionalized by different affinity ligands, and an application thereof in surface Plasmon optical affinity sandwich analysis. The surface of the extraction material is modified with a gold thin layer, and the surface of the gold thin layer is modified with an antibody, a molecularly imprinted polymer, nucleic acid or other affinity identification ligands or materials. A target analyte in a sample to be detected is specifically extracted to the surface of the extraction material by using the gold-based extraction material functionalized by different affinity ligands, the target analyte is labeled by using a silver-based nanometer Raman probe modified with the affinity ligand capable of identifying the target analyte to obtain a sandwich compound formed by the extraction material, the target compound and the Raman probe, and the content of the target analyte is detected through detecting a Raman signal. The extraction material has the advantages of high specificity, effective removal of interference of complex sample matrixes, high detection sensitivity reaching a single-molecular level, simple method and fast analysis speed, and can be used in living single cells, living animal analysis and immunological and biochemical analysis.

Provided are a high-stability, non-polarisation-dependent, surface-enhanced Raman scattering substrate, and the preparation and use thereof, wherein the preparation technique belongs to the field of material physical chemistry, the use thereof belongs to the fields of light scattering science and surface plasma science, and the substrate is a regular array formed by an equilateral trimer of gold nano-particles in a periodic arrangement. The preparation process involves: firstly, preparing, on an aluminium substrate, a layer of a periodic array of extremely thin alumina nano pit equilateral trimers by means of electrochemical corrosion; then, depositing a layer of extremely thin gold nano-film on the array; and then, annealing the nano pit array substrate with the gold film deposited thereon to obtain a gold nano-particle equilateral trimer periodic array, i.e. the high-stability, non-polarisation-dependent, surface-enhanced Raman scattering substrate. The substrate has the prominent advantage of all-angle stable output of a surface-enhanced Raman scattering signal within a full range of 360¡ã independent of an excitation light polarisation direction during the process of surface-enhanced Raman scattering detection.

The invention discloses a fluorescence and Raman detection device based on a lens group, and relates to the field of Raman detection. The device comprises a laser source, a lens group and a volume Bragg grating which are arranged in sequence, wherein the laser source is used for emitting laser to the lens group, the lens group is used for focusing the laser, the volume Bragg grating and the lasersource form a Fabry-Perot cavity, and a sample to be detected is arranged at a focus point of the lens group and used for generating Raman scattering according to the focused laser. According to the fluorescence and Raman detection device based on the lens group provided by the invention, the laser light source with relatively small energy is used, the Fabry-Perot gain cavity is formed between thevolume Bragg grating and the laser source to enhance laser, and the laser is focused through the lens group, so that the sample to be detected is enabled to be located at a focal point in the middleof the lens group, the laser emitted to the sample is enhanced, and a Raman scattering signal generated by the sample is also enhanced, thereby facilitating discrimination.

A preparing and measuring method of an achiral structure achieving circular dichroism is disclosed. The method includes subjecting a polystyrene sphere template to evaporation deposition with an A material, a B material, a C material and a D material in order according to a clockwise or anti-clockwise rotating manner, wherein the A material is an insulating material, the B material is a semiconductor material, the C material and the D material are metal materials, and conductivity of the A material, conductivity of the B material, conductivity of the C material and conductivity of the D material increase in order. The circular dichroism can be obtained through measuring on a sample of the achiral structure through a normal incidence process. A signal obtained when the circular dichroism is achieved through the achiral structure through the normal incidence process is more accurate than a signal obtained when the circular dichroism is achieved through traditional achiral structures through an oblique incidence process. The sample of the achiral structure prepared by the method can be used for enhancing chirality information of biomolecules and adopted as a sensor device.

The invention provides an active intracellular Raman spectrum detection method. The active intracellular Raman spectroscopy detection method includes: providing a thermal gradient generation layer, and setting cell fluid on the thermal gradient generation layer; injecting particles capable of exciting surface plasmons into the cell fluid; Inject the surfactant into the cell fluid; after injecting the surfactant into the cell fluid, use optical tweezers to introduce the particles into the cells in the cell fluid; introduce the particles into the cells Afterwards, the particle is controlled to move to a designated area in the cell by means of optical tweezers; after the particle moves to the designated area, Raman spectrum detection is performed on the cell. The Raman spectroscopy detection method induces particles that can excite surface plasmons into the cell, and the surface plasmons generated by the excited particles enhance the Raman signal of intracellular molecules, realizing intracellular particle-enhanced Raman scattering, Increased detection strength.

A nanoprobe device for Raman-enhanced protein detection and its preparation method, involving the fields of nanomaterials and life analysis chemistry. The core of the probe is polystyrene aldehyde-based microspheres with redox activity, and the surface is coated with silver nanometers. Particles, the outermost layer is adsorbed with protein molecules. The invention uses the surface-enhanced protein detection nano-probe designed to make full use of the surface plasmon interaction of silver nanoparticles to generate strong local electromagnetic field strength, which greatly enhances the Raman signal. At the same time, there are Ag-S bonds between these silver nanoparticles and proteins, and in the gaps between these silver nanoparticles, the amino groups on the protein are formed to react covalently with the aldehyde groups on the surface of polystyrene aldehyde-based microspheres, thereby immobilizing more A large amount of protein is of great help to detect low-concentration proteins. The nanoprobe has strong repeatability, low price and simple preparation.

The invention relates to a trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer, and belongs to the field of pesticide detection. The invention uses Raman active dye labeled gold nanoparticles as substrates, and uses the specific binding of pesticide and the aptamer binding to cause aggregation difference of reactive dye labeled gold nanoparticles in salt solution; a corresponding reactive dye SERS spectrum is obtained by a portable Raman spectrometer, so as to realize the quantitative detection of trace pesticide; and feasibility testing of the method is carried out in an actual tea sample. The method provided by the invention converts a conventional thinking of using the Raman characteristic peak of a target object to be detected as quantification determination in the traditional Raman technology into using the Raman characteristic peak of the reactive dye marker as the quantitative standard for target detection, so as to not only improve the detection sensitivity, simplify the preparation process of complex substrate, and provide convenience for research, inspection and supervision of trace pesticide residues in the fields of food industry.

The invention relates to enhanced Raman scattering substrates of silicon semiconductor which possessed good biological compatibility, a preparation method for the same, and the detection of rhodamine 6G molecules and 4-aminothiophenol molecules in solution with the said substrate. The invention adopts a chemical etching method to etch vertically-arranged silicon nanowire array in the surface of the monocrystalline silicon chip; then flocculent silver branches produced as by-products in the silicon nanowire array top in the etching process are removed; and a lot of Si-H bond are decorated in the nanowire surface. The enhanced Raman scattering substrates of silicon semiconductor are constituted by the vertically-arranged silicon nanowire array in the surface of the monocrystalline silicon chip and do not contain precious metal silver; the silicon nanowire surface is decorated with Si-H bonds. The invention realizes for the first time the preparation of enhanced Raman scattering substrates of silicon emiconductor which possessed good biological compatibility with only silicon materials rather than any precious metals.

The invention relates to a multiple-light field coupled Fano resonance metal plasmon resonance structure for selective enhancement, and a preparation method thereof. The resonance structure comprisesa metal film, a dielectric film and a metal hole array, wherein the material of the dielectric film is an optically transparent medium, and the dielectric film is located between the metal hole arrayand the metal film. The resonance structure can simultaneously realize a plurality of multi-wavelength resonances having extremely high electromagnetic field enhancement factors, the above resonance modes have a narrow line width and an equivalent resonance peak intensity, and a resonance light field is mainly localized to metal circular holes. The above characteristics allow the structure to simultaneously enhance the excitation field and the emission field of a target molecule in order to achieve selective detection of the target molecule while ensuring the high sensitivity and the high accuracy of detection. The preparation method of the resonance structure has a simple process, only requires a traditional nano-imprinting process and a thin film evaporation process, has a good repeatability and is convenient to apply.

The invention provides a Cu2O-Au composite microstructure surface enhanced Raman scattering active substrate and a production method thereof. The method comprises the following steps: chelating citrate ions and copper ions, shaping with polyvinylpyrrolidone, and carrying out a reaction under water bath conditions with glucose as a reducing agent to generate Cu2O octahedral crystals; and dispersing the Cu2O octahedral crystals in water, adding a AuCl<4><-> solution, reducing AuCl<4><-> by the Cu2O octahedral crystals to form Au nanoparticles, and carrying out Au nanoparticle in situ deposition on the surface of octahedral Cu2O to reduce the surface energy of a system and generate Cu2O-Au composite microparticles. A Cu2O-Au composite microstructure is designed and synthesized to develop the surface enhanced Raman scattering activity of a semiconductor Cu2O, and local surface plasma resonance of Au aggregate and strong electromagnetic field generated in an interface due to charge transfer between Cu2O and Au are used to improve the surface enhanced Raman scattering activity of the Cu2O-Au composite microstructure.

The invention belongs to the field of nanotechnology, and discloses a preparation method of triangular silver nanosheets. The triangular silver nanosheets prepared by the present invention have high purity, and have the stability of dark storage for at least 30 days under environmental conditions (the stability is measured by the displacement of the absorption peak wavelength of the ultraviolet-visible light spectrum by no more than 10nm); Thermal stability, the heat-resistant temperature can reach 95°C; it has a light-to-heat conversion efficiency of up to 42%; it has good biocompatibility, the material itself is neutral, it will not change the pH environment of the biological system, and has low biological toxicity; Under near-infrared light irradiation, a stable surface-enhanced Raman scattering signal can be generated.

The invention provides an active intracellular Raman spectrum detection method. The active intracellular Raman spectrum detection method comprises the following steps: providing a thermal gradient generation layer, and arranging cell sap on the thermal gradient generation layer; injecting particles capable of exciting surface plasmons into the cell sap; injecting a surfactant into the cell sap; after the surfactant is injected into the cell sap, introducing the particles into cells in the cell sap by adopting optical tweezers; after the particles are guided into the cells, controlling the particles to move to a designated area in the cells through optical tweezers; and after the particles move to the designated area, performing Raman spectrum detection on the cells. According to the Raman spectrum detection method, the particles capable of exciting the surface plasmons are induced to enter the cells, the surface plasmons generated by the exciting particles enhance Raman signals of molecules in the cells, particle enhanced Raman scattering in the cells is realized, and the detection intensity is improved.