An Active Intracellular Raman Spectroscopy Detection Method

Abstract

The invention provides an active intracellular Raman spectrum detection method. The active intracellular Raman spectroscopy detection method includes: providing a thermal gradient generation layer, and setting cell fluid on the thermal gradient generation layer; injecting particles capable of exciting surface plasmons into the cell fluid; Inject the surfactant into the cell fluid; after injecting the surfactant into the cell fluid, use optical tweezers to introduce the particles into the cells in the cell fluid; introduce the particles into the cells Afterwards, the particle is controlled to move to a designated area in the cell by means of optical tweezers; after the particle moves to the designated area, Raman spectrum detection is performed on the cell. The Raman spectroscopy detection method induces particles that can excite surface plasmons into the cell, and the surface plasmons generated by the excited particles enhance the Raman signal of intracellular molecules, realizing intracellular particle-enhanced Raman scattering, Increased detection strength.

Description

technical field [0001] The invention relates to the technical field of Raman signal sample detection, in particular to an active intracellular Raman spectrum detection method. Background technique [0002] Cells are the basic structural and functional units of organisms, composed of complex biomolecules wrapped in membranes, and these constituent molecules are distributed in different positions within the cell to achieve their respective functions. The precise detection and identification of these elements is an important issue in life science research. At present, methods for studying cell phenotypes at home and abroad include surface-enhanced Raman scattering, but the detection intensity of traditional Raman signals is too weak and the signal acquisition time is too long, which greatly affects limit its practical application. Contents of the invention [0003] Therefore, the technical problem to be solved by the present invention is to overcome the defect that the detec...

AI Technical Summary

Problems solved by technology

The precise detection and identification of these elements is an important issue in life science research. At present, the methods for studying cell phenotypes at home and abroad include surface-enhanced Raman scattering, but the traditional Raman signal detection intensity is too weak and the signal acquisition time is too long, which greatly affects limit its practical application

Images