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Trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer

A technology for surface-enhanced Raman and nucleic acid aptamers, which is applied in Raman scattering, measurement devices, material analysis by optical means, etc., to achieve the effects of low synthesis cost, simple operation process, and improved detection sensitivity

Active Publication Date: 2016-11-16
JIANGSU UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, a single nano-gold / silver colloid has been widely used as an enhanced substrate, but there are few reports on the detection of trace pesticide residues on Raman-enhanced substrates with markers combined with nucleic acid aptamers

Method used

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  • Trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer
  • Trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer
  • Trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer

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Embodiment Construction

[0022] The present invention has generality for trace pesticide residues, but due to the wide variety of pesticides, the present invention only selects acetamiprid, which is the most widely used among mainstream pesticides, as an implementation example, and the detection of other pesticides can refer to the method of this implementation example.

[0023] The example implementation steps are described in detail in conjunction with the accompanying drawings.

[0024] (1) Preparation of gold nanoparticles

[0025] Gold nanoparticles were prepared by trisodium citrate heating reduction method. First, use freshly prepared aqua regia (1:3HNO 3 / HCl) soak the glassware required in the preparation, rinse with distilled water before use, and use after drying in the air. Next, take 0.0200g of chloroauric acid and add 100ml of ultrapure water to prepare a 0.02% (M / V) solution and place it in a three-necked flask, and use an electric heating mantle to perform condensation and reflux hea...

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Abstract

The invention relates to a trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer, and belongs to the field of pesticide detection. The invention uses Raman active dye labeled gold nanoparticles as substrates, and uses the specific binding of pesticide and the aptamer binding to cause aggregation difference of reactive dye labeled gold nanoparticles in salt solution; a corresponding reactive dye SERS spectrum is obtained by a portable Raman spectrometer, so as to realize the quantitative detection of trace pesticide; and feasibility testing of the method is carried out in an actual tea sample. The method provided by the invention converts a conventional thinking of using the Raman characteristic peak of a target object to be detected as quantification determination in the traditional Raman technology into using the Raman characteristic peak of the reactive dye marker as the quantitative standard for target detection, so as to not only improve the detection sensitivity, simplify the preparation process of complex substrate, and provide convenience for research, inspection and supervision of trace pesticide residues in the fields of food industry.

Description

technical field [0001] The invention relates to a Raman spectrum detection method for pesticide residues, in particular to a surface enhanced Raman spectrum (SERS) detection method for trace pesticide residues based on nucleic acid aptamers, and belongs to the field of pesticide detection. Background technique [0002] Pesticides are widely used in agricultural production and cultivation at home and abroad due to their outstanding contributions in increasing crop income and preventing and controlling diseases and insect pests. However, when pesticides are ensuring the increase of vegetable income and grain yield, the phenomenon of pesticide residues also follows. The widespread and serious problem of pesticide residues brings not only ecological and environmental concerns, but more serious food safety hazards caused by the transfer of pesticide residues from nature into the body through passive ingestion or enrichment. At present, the detection methods of pesticides in my c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 胡薇薇陈全胜李欢欢张彬欧阳琴赵杰文
Owner JIANGSU UNIV
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