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A nanoprobe device for Raman-enhanced protein detection and its preparation method

A protein detection and nano-probe technology, which is applied in the field of life analysis chemistry and nano-materials, can solve the problems of low repeatability of the preparation method, high preparation cost, complicated operation, etc., and achieve simple process, high sensitivity and simple operation Effect

Active Publication Date: 2020-05-15
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of the above deficiencies, the purpose of the present invention is to propose a Raman-enhanced nanoprobe device for protein detection to solve the problems of low repeatability of the product manufacturing method, complicated operation, and high preparation cost

Method used

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  • A nanoprobe device for Raman-enhanced protein detection and its preparation method
  • A nanoprobe device for Raman-enhanced protein detection and its preparation method
  • A nanoprobe device for Raman-enhanced protein detection and its preparation method

Examples

Experimental program
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Embodiment 1

[0029] 1. Preparation of PS-CHO microspheres:

[0030] (1) Add 17.6mL of absolute ethanol, 20.8mL of deionized water and 2.1g of polyvinylpyrrolidone (PVP) into a three-necked flask equipped with a condenser, and place it in a constant temperature water bath at 70°C, mechanically stir and keep The rotating speed is 315r / min.

[0031] (2) Dissolve 0.1g of initiator AIBN in 6mL of styrene to obtain monomeric styrene in which initiator AIBN is dissolved.

[0032] (3) Add styrene monomer dissolved in initiator AIBN to the system obtained in (1), and add 6 mL of acrolein (C 3 h 4 O), after constant temperature reaction for 7 hours, add hydrochloric acid and increase the temperature of the constant temperature water bath to 80° C. for 12 hours, and then stop the reaction. Wash with ethanol / water and sonicate several times to obtain PS-CHO emulsion.

[0033] 2. Preparation of PS-CHO@Ag composite microspheres:

[0034] Take 0.02gAgNO 3 Dissolve in 1mL of deionized water, drop in...

Embodiment 2

[0044] The same steps as in Example 1 will not be repeated here, only the concentration of the BSA protein solution prepared in step 3 is changed: BSA protein is dispersed in 10mM phosphate buffered saline solution (PBS, pH7.2) to prepare 10-7 mol / L BSA solution.

[0045] Other steps are the same as in Example 1.

Embodiment 3

[0047] The same steps as in Example 1 will not be repeated here, only the concentration of the BSA protein solution prepared in step 3 is changed: the BSA protein is dispersed in 10 mM phosphate buffered saline solution (PBS, pH 7.2), and 10 -8 mol / L BSA solution.

[0048] Other steps are the same as in Example 1.

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Abstract

A nanoprobe device for Raman-enhanced protein detection and its preparation method, involving the fields of nanomaterials and life analysis chemistry. The core of the probe is polystyrene aldehyde-based microspheres with redox activity, and the surface is coated with silver nanometers. Particles, the outermost layer is adsorbed with protein molecules. The invention uses the surface-enhanced protein detection nano-probe designed to make full use of the surface plasmon interaction of silver nanoparticles to generate strong local electromagnetic field strength, which greatly enhances the Raman signal. At the same time, there are Ag-S bonds between these silver nanoparticles and proteins, and in the gaps between these silver nanoparticles, the amino groups on the protein are formed to react covalently with the aldehyde groups on the surface of polystyrene aldehyde-based microspheres, thereby immobilizing more A large amount of protein is of great help to detect low-concentration proteins. The nanoprobe has strong repeatability, low price and simple preparation.

Description

technical field [0001] The invention relates to the fields of nanomaterials and life analysis chemistry, in particular to the preparation technology of nanoprobe devices. Background technique [0002] Surface Enhanced Raman Spectrum (SERS) is an ultra-sensitive and fast general-purpose analytical tool, which has shown broad application prospects in the fields of environmental monitoring, biomedicine, and food safety. The signal intensity of surface-enhanced Raman spectroscopy is several to ten orders of magnitude higher than that of ordinary Raman spectroscopy, so surface-enhanced Raman scattering signals are used for the detection of extremely low concentrations or even single molecules, and when detecting organic molecules and biomolecules It shows the technical characteristics of being very simple, fast and cheap. [0003] However, a major obstacle in the practical application of SERS detection is the lack of an efficient and flexible method for the reproducible large-sc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/543G01N21/65
CPCG01N21/658G01N33/54346G01N33/68G01N2021/656
Inventor 严长浩严欣刘康宇
Owner YANGZHOU UNIV
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