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A surface-enhanced Raman spectroscopy detection method for trace pesticide residues based on nucleic acid aptamers

A surface-enhanced Raman and nucleic acid aptamer technology, which is applied in Raman scattering, measurement devices, and material analysis by optical means, to avoid fluorescence interference, low synthesis cost, and improve detection sensitivity.

Active Publication Date: 2019-01-04
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, a single nano-gold / silver colloid has been widely used as an enhanced substrate, but there are few reports on the detection of trace pesticide residues on Raman-enhanced substrates with markers combined with nucleic acid aptamers

Method used

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  • A surface-enhanced Raman spectroscopy detection method for trace pesticide residues based on nucleic acid aptamers
  • A surface-enhanced Raman spectroscopy detection method for trace pesticide residues based on nucleic acid aptamers
  • A surface-enhanced Raman spectroscopy detection method for trace pesticide residues based on nucleic acid aptamers

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Embodiment Construction

[0022] The present invention has versatility for trace pesticide residues, but because there are many types of pesticides, the present invention only selects acetamiprid, which is the most widely used in the mainstream pesticide category, as an implementation example, and the detection of other pesticides can refer to the method of this implementation example.

[0023] Example implementation steps are described in detail with reference to the accompanying drawings.

[0024] (1) Preparation of gold nanoparticles

[0025] The gold nanoparticles were prepared by the heating reduction method of trisodium citrate. First, freshly prepared aqua regia (1:3HNO 3 / HCl) soak the glassware used in the preparation, rinse with distilled water before use, and use after air drying. Next, get 0.0200g chloroauric acid and add 100ml ultrapure water, be mixed with 0.02% (M / V) solution and be placed in three-necked flask, and utilize electric heating mantle to carry out condensing reflux heating...

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Abstract

The invention relates to a trace pesticide residue surface enhanced Raman spectrum detection method based on a nucleic acid aptamer, and belongs to the field of pesticide detection. The invention uses Raman active dye labeled gold nanoparticles as substrates, and uses the specific binding of pesticide and the aptamer binding to cause aggregation difference of reactive dye labeled gold nanoparticles in salt solution; a corresponding reactive dye SERS spectrum is obtained by a portable Raman spectrometer, so as to realize the quantitative detection of trace pesticide; and feasibility testing of the method is carried out in an actual tea sample. The method provided by the invention converts a conventional thinking of using the Raman characteristic peak of a target object to be detected as quantification determination in the traditional Raman technology into using the Raman characteristic peak of the reactive dye marker as the quantitative standard for target detection, so as to not only improve the detection sensitivity, simplify the preparation process of complex substrate, and provide convenience for research, inspection and supervision of trace pesticide residues in the fields of food industry.

Description

technical field [0001] The invention relates to a Raman spectrum detection method for pesticide residues, in particular to a surface-enhanced Raman spectrum (SERS) detection method for trace pesticide residues based on nucleic acid aptamers, and belongs to the field of pesticide detection. Background technique [0002] Pesticides are widely used in agricultural production and cultivation at home and abroad because of their outstanding contributions to crop income increase and pest control. However, when pesticides ensure increased vegetable harvests and high grain yields, pesticide residues also follow. The widespread and serious problem of pesticide residues brings not only concerns about the ecological environment, but more serious food safety hazards caused by the transfer of residual pesticides from nature to the body through passive ingestion or enrichment. At present, my country's pesticide detection methods are mainly traditional detection methods, including: gas pha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陈全胜胡薇薇李欢欢张彬欧阳琴赵杰文
Owner JIANGSU UNIV
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