Method for verifying effectiveness of inhibition effect of health wine on helicobacter pylori

A technology for Helicobacter pylori and Helicobacter pylori, applied in biochemical equipment and methods, microbial determination/inspection, biological material analysis, etc., can solve problems such as not being scientifically verified, and achieve the effect of comprehensive inhibition effect

Inactive Publication Date: 2022-01-11
CHENGCHENG INVESTMENT GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many existing wine manufacturers advertise that their health wine has an inhibitory effect on Helicobacter pylori, but most of them have not been scientifically verified

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] A method for verifying the effectiveness of health-preserving wine for inhibiting Helicobacter pylori, comprising the following steps:

[0040] (1), prepare sufficient international standard strains of Helicobacter pylori, prepare 70 experimental mice, 18-22g, half male and half male;

[0041] (2) Grouping: All mice were randomly divided into 7 groups, namely blank control group, model control group, positive drug group, high-dose health wine group, low-dose health wine group, high-dose ordinary liquor group, low-dose ordinary liquor Dosage group, 10 in each group, and then the experimental mice were first adaptively fed for a week;

[0042](3) Bacterial culture: inoculate the Helicobacter pylori strains on the Columbia blood agar plate with the amount of 100ul bacterial suspension on each plate in the ultra-clean workbench. After the bacteria solution is sucked dry on the medium, culture The base was placed upside down in the anaerobic culture bag, and then the microa...

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Abstract

The invention discloses a method for verifying the effectiveness of inhibition effect of health wine on helicobacter pylori. The method specifically comprises the following steps: preparing a helicobacter pylori strain and experimental mice; grouping the experimental mice; carrying out bacterial culture and animal modeling; dosing the grouped mice; performing model detection and model replication evaluation; performing physical sign observation on the mice; dissecting the mice, carrying out general morphological observation on the stomachs of the mice, carrying out urease detection on gastric antrum mucosa tissue, and carrying out gastric histopathologic HE staining and hepatic histopathologic HE staining; and judging the effectiveness of the inhibition effect of the health wine on the helicobacter pylori according to a sign observation result, a stomach general morphological observation result, a gastric sinus mucosa tissue urease detection result, an observation result after gastric histopathologic HE staining and an observation result after hepatic histopathologic HE staining. The verification method can be used for scientifically, effectively and comprehensively verifying the helicobacter pylori inhibition effect of the health wine.

Description

technical field [0001] The invention belongs to the technical field of bacteria inhibition, and in particular relates to a method for verifying the effectiveness of health-preserving wine's inhibitory effect on Helicobacter pylori. Background technique [0002] Helicobacter pylori is a Gram-negative, microaerophilic bacterium that lives in various regions of the stomach and duodenum. It can cause mild chronic inflammation of the gastric mucosa and even lead to gastric and duodenal ulcers and gastric cancer. Alcohol with a moderate amount of alcohol in life has a certain inhibitory effect on bacteria, and high-concentration alcohol is used for disinfection in medicine. Many existing wine manufacturers advertise that their health wine has an inhibitory effect on Helicobacter pylori, but most of them have not been scientifically verified. Therefore, a verification method is needed to verify the effectiveness of alcohol in inhibiting Helicobacter pylori. Contents of the inve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/84G01N1/30G01N1/36C12Q1/18C12Q1/58
CPCG01N21/84G01N1/30G01N1/36C12Q1/58C12Q1/18G01N2333/195G01N2333/98
Inventor 白玉锋
Owner CHENGCHENG INVESTMENT GRP CO LTD
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