Construction and identification method of corn Puccinia polysora Underw. pathotype differential host system

Abstract

The invention provides a construction and identification method of a corn Puccinia polysora Underw. pathotype differential host system. The method comprises the following steps: collecting seeds and seedlings; collecting strains: collecting the tested corn southern rust strains from corn southern rust disease samples in multiple fields, wherein except that strains in one field are subjected to artificial propagation in a greenhouse and uredospores are collected for later use, strains in other regions are obtained for later use by directly scraping uredospores from field diseased strain samples; carrying out inter-field inoculation on the collected strains in a corn large horn mouth period; selecting and inoculating strains in different regions according to disease indexes after diseases occur in the field, and obtaining a corn variety which is susceptible to diseases and resistant to diseases and is resistant to diseases by integrating bacterial sources in multiple regions; conducting secondary purification, and culturing a plurality of monospore systems in various regions; inoculating the screened disease-resistant variety with a plurality of secondarily purified single spore systems, and selecting a disease-resistant and susceptible corn variety; classifying pathotypes by adopting a binary accumulation method; and finding out the pathotype of corn Puccinia polysora Underw. with the highest frequency in China.

Description

technical field [0001] The invention relates to a method for identifying a pathogenic type host of corn rust fungus, in particular to a method for constructing and identifying a pathogenic type host system of Chinese corn rust fungus. Background technique [0002] Southern rust of corn is an airborne disease caused by Puccinia polysora Underw. The main damage parts of corn rust are the leaves and leaf sheaths of corn, and in severe cases, it can also infect the corn stalks and bracts. When the disease is severe, a large number of spores will be scattered on the leaves, killing the host tissue, causing the leaves to dry up and die early, especially on late-seeded corn. In the early stage of the disease, chlorotic spots will first appear near the infection point, and then There will be herpes-like raised spore piles, and the spore piles can be produced on the front and back of the leaves, usually on the leaf surface. Buried under the host's epidermis, many pale yellow spores...

AI Technical Summary

Problems solved by technology

However, the research in our country is in its infancy, and the types or distribution of physiological races and pathogenic types are still unclear. Most of the research in my country has not considered the existence of multiple physiological races or differences in pathogenicity, and cannot be based on physiological races or advantages At the level of pathogenic types, there is no basis for improving the overall rust resistance of maize varieties by using horizontal disease resistance, vertical disease resistance, adult plant disease resistance, and slow rust. Status combination of physiological race or dominant pathogenic type

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