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Identification method of traditional Chinese medicinal material poria with hostwood

A technology of traditional Chinese medicinal materials and Fushen, which is applied in the field of identification of Chinese medicinal materials, can solve the problems that it cannot be used to distinguish Fushen from Fuling medicinal materials, cannot effectively reflect the specificity of Fushen medicinal materials, and there is no pharmacopoeia standard for Fushen medicinal materials.

Pending Publication Date: 2022-03-08
浙江双石药业有限公司
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AI Technical Summary

Problems solved by technology

[0004] There is no pharmacopoeia standard for the existing Fushen medicinal materials, and the current effective standards use Poria cocos as a reference medicinal material for identification, which cannot effectively reflect the specificity of Fushen and cannot be used to distinguish Fushen from Poria medicinal materials

Method used

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  • Identification method of traditional Chinese medicinal material poria with hostwood
  • Identification method of traditional Chinese medicinal material poria with hostwood

Examples

Experimental program
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Effect test

Embodiment

[0020] The identification method of the Chinese herbal medicine Fushen, in this identification example, five batches of the medicinal material Fushen were tested by thin-layer chromatography.

[0021] The detection method is as follows:

[0022] 1. Five batches of the medicinal material Fushen to be tested were crushed for use;

[0023] 2. Take 1 g of the powder prepared in step 1, add 50 ml of diethyl ether, plug it tightly, extract it ultrasonically for 10 minutes, filter, evaporate the filtrate to dryness, add 1 ml of methanol to the residue to dissolve it, and use it as the test solution;

[0024] 3. Take another 1 g of the reference medicinal material Fushen, and make a reference medicinal solution in the same way as in steps 1 and 2;

[0025] 4. Thin-layer chromatography test, draw 5 μl of each of the above solutions, spot them on the same silica gel G thin-layer plate, use toluene-ethyl acetate-formic acid with a mass ratio of 20:5:0.5 as the developer, develop, take o...

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Abstract

The invention discloses a method for identifying a traditional Chinese medicinal material poria with hostwood. Comprising the following steps: crushing to-be-detected medicinal material poria with hostwood for later use; taking 1g of the prepared powder, adding 50ml of diethyl ether, sealing and plugging, performing ultrasonic extraction for 10 minutes, filtering, evaporating filtrate to dryness, and adding 1ml of methanol into residues to dissolve to obtain a test solution; preparing 1g of reference medicinal material poria with hostwood into a reference medicinal material solution; testing by thin-layer chromatography, sucking 5 times of the two solutions, respectively dripping the two solutions on the same silica gel G thin-layer plate, developing, taking out and airing by taking toluene-ethyl acetate-formic acid in a mass ratio of 20: 5: 0.5 as a developing solvent, spraying a 2% vanillin sulfuric acid-ethanol mixed solution, and heating at 105 DEG C until spots are clearly displayed; main spots with the same color or not are displayed at the position corresponding to the color spectrum of the reference medicinal material poria cocos. The method is beneficial to qualitative identification of the poria with hostwood medicinal material, can distinguish the poria with hostwood and the poria cocos medicinal material, can effectively determine the specificity of the poria with hostwood and the poria cocos in the research of the poria with hostwood decoction preparation, and lays a foundation for the research of the effective components of the subsequent decoction.

Description

technical field [0001] The invention belongs to the technical field of identification of traditional Chinese medicinal materials, and relates to an identification method of the traditional Chinese medicinal material Fushen. Background technique [0002] Fushen Decoction is used to soothe the nerves and calm the mind, lack of heart-qi, palpitations due to deficiency, daily crying, birth of a baby, emaciation and many frights and other diseases. The main component of Fushen Decoction includes the traditional Chinese medicine Fushen. [0003] Fushen is the part with pine roots in the sclerotia of Polyporaceae Poria genus Poria. The original species is a lower plant and is a fungus parasitic on pine trees. The medicinal part is the white part with pine roots in the middle of the dry sclerotium. Nature and flavor are sweet, flat. It has the functions of expelling dampness, invigorating the spleen, and calming the mind. For phlegm retention, edema, dysuria, diarrhea, heart pal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 赵莉左帅王嫣然
Owner 浙江双石药业有限公司
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