Identification method of traditional Chinese medicinal material poria with hostwood
A technology of traditional Chinese medicinal materials and Fushen, which is applied in the field of identification of Chinese medicinal materials, can solve the problems that it cannot be used to distinguish Fushen from Fuling medicinal materials, cannot effectively reflect the specificity of Fushen medicinal materials, and there is no pharmacopoeia standard for Fushen medicinal materials.
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[0020] The identification method of the Chinese herbal medicine Fushen, in this identification example, five batches of the medicinal material Fushen were tested by thin-layer chromatography.
[0021] The detection method is as follows:
[0022] 1. Five batches of the medicinal material Fushen to be tested were crushed for use;
[0023] 2. Take 1 g of the powder prepared in step 1, add 50 ml of diethyl ether, plug it tightly, extract it ultrasonically for 10 minutes, filter, evaporate the filtrate to dryness, add 1 ml of methanol to the residue to dissolve it, and use it as the test solution;
[0024] 3. Take another 1 g of the reference medicinal material Fushen, and make a reference medicinal solution in the same way as in steps 1 and 2;
[0025] 4. Thin-layer chromatography test, draw 5 μl of each of the above solutions, spot them on the same silica gel G thin-layer plate, use toluene-ethyl acetate-formic acid with a mass ratio of 20:5:0.5 as the developer, develop, take o...
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