Gel composition, biological scaffold gel as well as preparation method and application of biological scaffold gel

Abstract

The invention provides a gel composition, biological scaffold gel as well as a preparation method and application of the biological scaffold gel. The gel composition is prepared from polyethylene glycol (PEG), collagen, laminin, RGD polypeptide, B27, N2, N-acetylcystine, Nicotinamide, a Wnt signaling pathway agonist, an epidermal growth factor and a ROCK inhibitor. The biological scaffold gel comprises the following components according to final concentration: 0.01-0.5 g / ml of PEG (Polyethylene Glycol); the content of collagen is 0.02 to 0.5 g / ml; the concentration of the laminin is 0.05 to 100 ng / ml; b27, 0.5 to 5 *; n2, 0.5 to 5 *; the concentration of the N-acetylcystine is 0.1 to 50 [mu] M; the concentration of the Nicotinamide is 10 to 200 [mu] M; the concentration of the Wnt signal channel agonist is 25 to 500 ng / ml; eGF (Epidermal Growth Factor) with the concentration of The concentration of the ROCK inhibitor is 0.5 ng / ml to 100 ng / ml; the concentration of the RGD polypeptide is 0.1 mg / ml to 1 mg / ml; all the components are dissolved in a buffer solution or a basic culture medium. According to the method, 3D culture of cells and tissues can be efficiently carried out, and formed 3D cultures such as cell clusters and organoids can simulate in-vivo three-dimensional tissue structures and gene expression characteristics.

Description

technical field [0001] The invention relates to the technical field of organoid culture, in particular to a gel composition, a biological scaffold gel and a preparation method and application thereof. Background technique [0002] Organoids are a three-dimensional cell culture technology that can mimic the tissue structure and gene expression of in vivo organs in vitro. Organoids are a variety of cells including stem cells that undergo self-differentiation and self-assembly in vitro to form three-dimensional aggregates. The cell types, ratios, and spatial structures and arrangements of cells are highly similar to those in vivo and can simulate in vivo Some functions of organs. Furthermore, gene expression in organoids is closer to in vivo than in traditional 2D cell culture. Therefore, the results of organoid-based bioactivity assays are more relevant in vivo. Therefore, organoids are currently widely used in biological research, drug research, medical research, and the t...

AI Technical Summary

Problems solved by technology

Collagen has strong biocompatibility, but due to the lack of other materials such as cytokines, its culture effect is still not as good as matrigel from biological sources. In addition, pure collagen components are tightly cross-linked, which increases the ability of the gel to dissolve and recover cells. difficulty

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