Mass spectrometry kit and method for screening and prognosis evaluation of lung cancer

Abstract

The invention discloses a mass spectrometry kit for lung cancer screening and prognosis evaluation, which comprises a CEA antibody, a CA125 antibody, an SCC antibody, an NSE antibody, a Pro-GRP antibody, a CYFRA21-1 antibody and a solid phase carrier, the CEA antibody, the CA125 antibody, the SCC antibody, the NSE antibody, the Pro-GRP antibody and the CYFRA21-1 antibody are respectively fixed on the solid phase carrier, and the solid phase carrier is fixed on the solid phase carrier. The CEA antibody, the CA125 antibody, the SCC antibody, the NSE antibody, the Pro-GRP antibody and the CYFRA21-1 antibody are combined on the solid-phase carrier in a non-covalent connection manner, and the solid-phase carrier is polystyrene particles coated with protein A. The lung cancer detection kit disclosed by the invention is higher in specificity, better in early-stage lung cancer screening effect and suitable for screening all-stage lung cancer patients.

Description

technical field [0001] The invention relates to the technical field of lung cancer detection, in particular to a mass spectrometry kit and method for lung cancer screening and prognosis assessment. Background technique [0002] Lung cancer is one of the malignant tumors with the fastest increasing morbidity and mortality in my country and the greatest threat to the health and life of the population. At present, lung cancer has accounted for the first place in the incidence and mortality of male malignant tumors. The reason is that on the one hand, the incidence of lung cancer continues to rise due to factors such as the increase in smoking population and environmental pollution, and on the other hand, there is a lack of effective treatments. The method and technology of early screening of lung cancer, the vast majority of lung cancer patients can only be diagnosed in the late stage, resulting in the loss of the best period of treatment for most patients. [0003] Tumor mark...

AI Technical Summary

Problems solved by technology

[0012] However, the existing lung cancer detection kits have the following problems: 1. The existing lung cancer detection kits are of various types, including a kit for lung adenocarcinoma detection, a kit for small cell lung cancer detection, and a non-small cell lung cancer detection kit. The kits for lung cancer detection, etc., do not contain all the above-mentioned lung cancer markers at the same time. During the general screening of lung cancer, there are many types of lung cancer patients, and the above-mentioned different and single types of lung cancer detection kits are used for In the screening of lung cancer screening, it will undoubtedly greatly increase the cost of lung cancer screening, and increase the detection procedures and steps of lung cancer screening, resulting in low screening efficiency of lung cancer screening; 2. There are many lung cancer marker antigens in the existing lung cancer detection kits It is prepared by monoclonal antibody, and there are many kinds of lung cancer markers at present. Each lung cancer marker antigen is prepared by monoclonal antibody preparation method, which will undoubtedly increase a lot of repetitive work and reduce the preparation efficiency of the kit.

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