Separation and identification equipment for natural killer cells of peripheral blood and preparation method of culture medium

Abstract

The invention discloses peripheral blood natural killer cell separation and identification equipment which comprises a separation and identification equipment body, leveling mechanisms are mounted at the four corners of the bottom of the separation and identification equipment body respectively, a servo motor is mounted on one side of the inner bottom face of the separation and identification equipment body, and the output end of the servo motor is fixedly connected with a rotating disc. Through the arrangement of the angle adjusting mechanism, angle adjustment of the test tube fixing cylinder is achieved, the centrifugal angle and the centrifugal radius of a centrifugal tube can be adjusted according to the actual situation, the centrifugal efficiency is improved, through installation of the leveling mechanism, the centrifugal tube fixing cylinder can be conveniently adjusted, and the centrifugal tube fixing cylinder is convenient to adjust. According to the present invention, the four corners of the equipment are leveled, the horizontal installation of the equipment is easily ensured, and the purity of the peripheral blood natural killer cells is 90-95% and the amplification speed of the cells is 600-700 times during the culture process of the peripheral blood natural killer cells by using the culture medium prepared by the present invention.

Description

technical field [0001] The invention relates to the technical field of separation and identification of natural killer cells in peripheral blood, in particular to equipment for the separation and identification of natural killer cells in peripheral blood and a preparation method for a culture medium. Background technique [0002] Peripheral blood natural killer cells are natural killer cells in blood other than bone marrow blood. Natural killer cells are important immune cells in the body, not only related to anti-tumor, anti-viral infection and immune regulation, but also in some cases Participate in the occurrence of hypersensitivity reactions and autoimmune diseases, and be able to identify target cells and killing mediators. Separation and identification equipment is an essential part of the isolation and identification of peripheral blood natural killer cells; [0003] There are many types of such separation and identification equipment on the market today, which can ba...

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