Formula and preparation method of antibacterial enzyme

A production method and enzyme technology, which can be applied in the directions of adding food elements, resisting vector-borne diseases, forming or processing animal feed, etc., can solve the problems of single structure, short half-life of growth hormone, weak effect of feed on animals, etc. Action time, prolong half-life, and promote the effect of animal growth

Inactive Publication Date: 2022-06-03
河北普农文化传播有限公司
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Problems solved by technology

[0005] In order to make up for the deficiencies of the prior art and solve the problem of growth hormones that play an effective role in animal growth, generally they can only promote the growth of animals, and because the structure is relatively single, the half-life of growt

Method used

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  • Formula and preparation method of antibacterial enzyme
  • Formula and preparation method of antibacterial enzyme
  • Formula and preparation method of antibacterial enzyme

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Experimental program
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Embodiment approach

[0059] As an embodiment of the present invention, in the S2, the preparation of lysozyme is generally prepared by egg white, and the main operation step is to add egg white to 724 resin for washing, leave standing overnight, use buffer to elute, and then pass sulfuric acid. The ammonium solution is salted out and dialyzed to remove the basic protein, and the dialysate clear liquid is obtained, and sodium hydroxide solution is added to the dialyzed clear liquid, and finally the pH is adjusted with hydrochloric acid, and the white flake lysozyme is obtained after freeze-drying.

[0060] Specifically, the preparation of lysozyme is mainly by adding 540kg of fresh egg whites to the treated 80kg 724 resin at 5-10°C, stirring and adsorbing for 6h, standing at 0-5°C overnight, and then pouring out the upper egg whites, The resin was centrifuged and dried, and the adhered egg protein was repeatedly washed with distilled water, and then the resin was loaded into the column, and the resi...

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Abstract

The invention belongs to the technical field of additives, and particularly relates to an antibacterial enzyme formula and a preparation method, the antibacterial enzyme formula comprises the following components: phytosterol, lysozyme, total arsenic and lead; wherein the phytosterol is beta-sitosterol, and the content of the beta-sitosterol is greater than or equal to 3000mg/kg; the activity of the lysozyme is greater than or equal to 6000 U/g; the content of the total arsenic is less than or equal to 10.0 mg/kg; the content of lead is less than or equal to 40.0 mg/kg; when the antibacterial enzyme prepared from the phytosterol and the lysozyme is used in cooperation with the feed for promoting growth, in a water solute state, the phytosterol, the growth hormone in the feed and a ribonucleoprotein body form a complex, compared with the traditional growth hormone independently existing, the complex is relatively stable, the half-life period of the growth hormone is prolonged, and the growth of the feed is promoted. The effect of stabilizing the growth hormone structure is fully exerted, the action time of the growth hormone is prolonged, and the growth of animals is promoted.

Description

technical field [0001] The invention belongs to the technical field of additives, in particular to a formula and a preparation method of an antibacterial enzyme. Background technique [0002] Enzymes refer to enzymes, which play a certain role in the growth of animals and plants. [0003] Animals need to be fed regularly during the feeding process, and adding additives to the feed has a beneficial effect on animal growth; generally, adding growth hormone to the feed is the most common. [0004] Growth hormone that plays an effective role in animal growth can generally only promote animal growth, and due to its relatively single structure, the half-life of growth hormone is short, and the effect of growth hormone cannot be fully exerted, which in turn leads to the effect of feed on animal growth. The effect is relatively weak; therefore, the present invention provides an antibacterial enzyme formula and a preparation method. SUMMARY OF THE INVENTION [0005] In order to m...

Claims

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Application Information

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IPC IPC(8): A23K20/189A23K20/168A23K20/20A23K40/00
CPCA23K20/189A23K20/30A23K20/168A23K40/00Y02A50/30
Inventor 赵俊荣陆云鹏
Owner 河北普农文化传播有限公司
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