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Application of marker and detection reagent thereof in preparation of kit for screening and/or auxiliary diagnosis of ASD

An auxiliary diagnosis and marker technology, applied in the medical field, can solve the problems of little correlation between disease severity, inability to establish a clear pathogenesis, and low accuracy.

Pending Publication Date: 2022-07-29
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing ASD flora characteristics are mostly obtained from the analysis of the abundance difference of the common metagenomic group, which has little correlation with the severity of the disease, cannot establish a clear pathogenesis, has low accuracy, and has a large number of false positives and false negatives

Method used

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  • Application of marker and detection reagent thereof in preparation of kit for screening and/or auxiliary diagnosis of ASD
  • Application of marker and detection reagent thereof in preparation of kit for screening and/or auxiliary diagnosis of ASD
  • Application of marker and detection reagent thereof in preparation of kit for screening and/or auxiliary diagnosis of ASD

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 Oral Streptococcus and Oral Streptococcus Phage Abundance Detection

[0028] A control population of 26 children with ASD and 26 sex- and age-matched typically developing children was recruited for this study. All participants were diagnosed according to the Diagnostic and Statistical Manual of Mental Disorders, 5th Edition, based on ADOS (AutismDiagnostic Observation Schedule), CARS (Rating or scoringinstruments of Childhood Autism Rating Scale, Childhood Autism Rating Scale), RBS-R (Repetitive Behavior Scale-Revised, stereotyped repetitive behavior scale) and ABC (Autism Behavior Checklist, Autism Behavior Scale) scale for behavioral assessment. For the control group, physical examinations and parent interviews were performed to rule out any psychiatric disorders. Participants were excluded if they developed an infection or took psychotropic medications, antibiotics, probiotics, prebiotics, or a special diet in the three months prior to enrollment. Prof...

Embodiment 2

[0030] Example 2 Correlation between oral Streptococcus phage, Streptococcus bacteria and clinical manifestations of autism

[0031] The total ADOS score consists of scores in four domains: (1) Language and Communication (LC); (2) Reciprocal Social Interaction (RSI); (3) Stereotyped Behavior and Restricted Interests (SBRI); (4) Play, Score Higher values ​​indicate more severe clinical manifestations. After Spearman correlation analysis, the results are as follows Figure 5 As shown, the abundance of streptococcal phage was strongly positively correlated with the ADOS assessment total score and LC score ( Figure 5 A, rho = 0.52, rho 0.65, respectively). Second, the abundance of Streptococcal VFs was also positively correlated with the total ADOS and LC scores ( Figure 5 B, rho=0.35, rho=0.49, respectively). The total abundance of Streptococcus bacteria showed a strong positive correlation with the ADOS total score and LC score ( Figure 5 C, rho=0.55, rho=0.42, respectiv...

Embodiment 3

[0032] Example 3 Construction of Random Forest Classification Model for ASD Prediction

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Abstract

The invention provides application of a marker and a detection reagent thereof in preparation of a kit for screening and / or auxiliary diagnosis of ASD. Studies find that the abundance of Streptococcus oral cavity, the abundance of Streptococcus oral cavity bacteriophage and the abundance of virulence factors (VFs) carried by Streptococcus oral cavity have significant differences in ASD and normal children's oral cavities, and are closely related to the severity of clinical manifestations such as ADOS score and language exchange score, the changes are not observed in parallel intestinal flora sampling, and the abundance of Streptococcus oral cavity bacteriophage and VFs carried by the Streptococcus oral cavity bacteriophage and the VFs carried by the Streptococcus oral cavity bacteriophage have significant differences in ASD and normal children's oral cavities and are closely related to the severity of clinical manifestations such as ADOS score and language exchange score. The result shows that an unprecedented oral specific association exists between the ASD pathogenesis and excessive enrichment of the streptococcal bacteriophage, and the ASD pathogenesis and excessive enrichment of the streptococcal bacteriophage are closely related to the severity of the disease.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular to the application of markers and their detection reagents in the preparation of kits for screening and / or auxiliary diagnosis of ASD. Background technique [0002] Autism is a complex neurodevelopmental disorder that appears in the first few years of life and can lead to lifelong disability. In recent years, the incidence of ASD has increased significantly. However, because the etiology and pathogenesis of ASD are not fully understood, there is currently a lack of effective physiological diagnosis and medical interventions. The traditional ASD diagnosis is made only by behavioral scales (CARS, DSM-5, ABC, ADI-R, ADOS, etc.), which is based on a comprehensive judgment of observation and questionnaire scores, and lags compared with physiological changes. Although diagnostic markers based on blood and urine metabolites, biological enzymes, and intestinal flora have appeared,...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/689C12Q1/6888G01N33/68C12N15/11C12R1/46C12R1/92
CPCC12Q1/6883C12Q1/689C12Q1/6888G01N33/6896G01N2800/30G01N2800/18G01N2333/005G01N2333/315
Inventor 康禹楚亚男邵长君王建陈婧
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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