Method for separating silk gland cell of silkworm suited to researches in molecular biology

A technology of molecular biology and separation methods, applied in the fields of biochemical equipment and methods, animal cells, microorganisms, etc.
CN1220772CInactive Publication Date: 2005-09-28ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2005-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

A process for separating silk gland cells of silkworm includes dissecting young silkworm in precooled physiological saline to obtain silk glands, washing, immersing in precooled ethanol or methanol, scraping silk gland cels, drying, and extracting DNA specimen and protein specimen.
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Description

Technical field

[0001] The invention relates to a silk gland cell separation method suitable for molecular biology research. Background technique

[0002] Bombyx mori silk glands are a pair of tubular glands that synthesize and secrete silk, and are the material basis for cocoon production. Morphologically, they can be clearly divided into three parts: the anterior silk gland, the middle silk gland and the posterior silk gland. The middle silk gland secretes sericin, and the posterior silk gland secretes silk fibroin. By studying the genes, protein composition and function of silk gland cells, it will help to discover functional proteins and functional genes related to the secretion of silk proteins by silk gland cells, understand the mechanism of high-yield silkworm cocoons, and lay the foundation for improving the production capacity of silkworms; Glandular cells are used as a model to study the mechanism of action of eukaryotic gene expression regulation. However, since...

Claims

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