Nucleic acids encoding proteins involved in sensory transduction

一种核酸、编码的技术，应用在动物/人类蛋白质、抗动物/人类的免疫球蛋白、抗受体/细胞表面抗原/细胞表面决定因子免疫球蛋白等方向

Inactive Publication Date: 2001-10-10
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the specific membrane receptors involved in taste transduction, or the many individual intracellular signaling molecules activated by individual taste transduction pathways

Method used

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Examples

Experimental program
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Effect test

Embodiment I

[0239] Embodiment 1: Cloning and expression of TCP#1-#3

[0240] A cDNA library of rat peripheral and fungiform single cells was used to clone and isolate taste cell-specific nucleic acids of the invention.

[0241]Single taste receptor cells were isolated from dissociated peripheral papillae as described by Bernhardt et al., J. Physiol. 490:325-336 (1996). Single cells isolated from 20 rat papillae (batches of 20) yielded 280 single cell cDNA populations according to the method of Dulac & Axel, Cell 83: 195-206 (1995). Southern and dot blot analysis of amplified single-cell cDNA probed with radiolabeled probes to identify similar cell types. Gustduein, a G protein expressed specifically in a subset of taste receptor cells, was chosen as a marker for taste cells (McLaughlin et al., supra). Cell integrity was confirmed with Tubulin and N-Cam, and amplification reactions were verified.

[0242] A lambda phage cDNA library was then constructed from each Gustducin-positive cell...

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Abstract

The present invention provides isolated nucleic acid and amino acid sequences of sensory cell-specific polypeptides, antibodies to these polypeptides, methods for detecting these nucleic acids and polypeptides, and methods for screening for modulators of sensory cell-specific polypeptides.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to USSN 60 / 094,464, filed July 28, 1998, which is incorporated herein by reference. [0003] Statement Regarding Federally Funded Research and Development [0004] This invention is funded by the government grant number 5R01DC03160 and the Ministry of Health. The government has certain rights in this invention. field of invention [0005] The present invention provides isolated nucleic acid and amino acid sequences of sensory cell-specific polypeptides, antibodies to these polypeptides, methods of detecting these nucleic acids and polypeptides, and methods of screening for modulators of sensory cell-specific polypeptides. Background of the invention [0006] Taste transduction is one of the most complex forms of chemical transduction in animals (see eg, Margol skee, Biology Papers 15:645-65...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K14/705C07K16/28C12N1/15C12N1/19G01N33/50C12N1/21C12N5/10C12N15/09C12P21/02C12Q1/02C12R1/91G01N33/15G01N33/53G01N33/566
CPCC07K14/47C12N15/11
Inventor C·S·朱克J·林德迈耶D·科恩J·E·阿德莱尔
Owner RGT UNIV OF CALIFORNIA
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