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Nucleic acids encoding G protein gamma subunit involved in sensory transduction

A technology of G protein and γ subunit, applied in the field of nucleic acid encoding G protein γ subunit related to sensory transduction

Inactive Publication Date: 2001-11-07
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, little is known about the specific membrane receptors involved in taste transduction, or the many intracellular individual signaling molecules activated by individual taste transduction pathways

Method used

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Examples

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Embodiment

[0236] The following examples are offered by way of illustration only, not limitation. Those skilled in the art will readily appreciate that various noncritical parameters can be changed or modified to obtain substantially similar results.

[0237] Example I: TC-G γ Cloning and expression of

[0238] Isolation of TC-G of the present invention using a cDNA library generated from rat peripheral single cells γ nucleic acid.

[0239] Single taste receptor cells were isolated from exfoliated peripheral papillae of the rat tongue. Generation of 250 single-cell cDNA libraries from single cells isolated from 20 rat papillae (20 per batch) (see, e.g., Berhhardt et al., J. Physiol. 490:325-336 (1996); Dulac & Axel, Cell 83:195 -206 (1995)). Amplified single cell cDNA was analyzed by DNA and dot blot and probed with probes selected to identify likely similar cell types. Gustducin, a G protein expressed specifically in a subset of taste receptor cells, was chosen as a taste cell mar...

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Abstract

The present invention provides isolated nucleic acid and amino acid sequences of sensory cell-specific G protein gamma subunits, antibodies to these subunits, methods for detecting these nucleic acids and subunits, and screening for modulators of sensory cell-specific G protein gamma subunits Methods.

Description

[0001] Cross References to Related Applications [0002] This application claims priority from USSN 60 / 102,480, filed September 30, 1998, which is incorporated herein by reference. [0003] Statement Regarding Federally Funded Research and Development [0004] This invention was made with government support, Grant No. 5R01DC03160, awarded by the National Institutes of Health. The government therefore has certain rights in the invention. field of invention [0005] The present invention provides isolated nucleic acid and amino acid sequences of sensory cell-specific G protein gamma subunits, antibodies to these subunits, methods for detecting these nucleic acids and subunits, and screening for modulators of sensory cell-specific G protein gamma subunits Methods. Background of the invention [0006] Taste transduction is one of the most complex forms of chemical transduction in animals (see eg, Margolskee, Biology Papers 15:645-650 (1993); Avenet & Lindemann, J. Membrane Bi...

Claims

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Application Information

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IPC IPC(8): C07K14/47
CPCC07K14/47C07K14/4722C12N15/11
Inventor C·S·朱克J·E·阿德莱尔D·科恩
Owner RGT UNIV OF CALIFORNIA
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