Herbicid resistant rice
A herbicide and resistance technology, applied in the field of imidazolinone herbicide and sulfonylurea herbicide resistant rice, can solve the problem of no given strain
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Embodiment 1-15
[0034] Approximately 52 million mutant (M 2 ) rice seeds. By adding a total of 340 pounds of rice cultivar "Cypress" or "Bengal" seed (M 1 ) soaked in 0.175% (by weight) EMS aqueous solution to develop mutant seeds. About 170 lbs of rice seeds were exposed to EMS for 16 hours; about 85 lbs for 24 hours; and about 85 lbs for 35 hours. Seeds from the three exposure regimes were collected for the screening experiments described below.
[0035] After EMS treatment, rinse the M thoroughly with water 1 Seeds, drained before sowing in shallow water by broadcast, and drained after 24 hours. Three days later, the farmland was flooded again, and the farmland was kept flooded until it was drained for harvesting. Harvest M 2 Seeds are stored for the winter and screened for herbicide resistance the following year. Punch and sow about 52 million grains of M 2 After seeding, imazethapyr was applied at a rate of 0.125 lb ai / A (pounds of active ingredient per acre) prior to seed emerge...
Embodiment 16-27
[0043] There are about 60 million additional mutant M 2 Rice seeds are screened. By adding a total of 300 pounds of seeds of the rice cultivar °Cypress (M 1 ) soaked in 0.175% (by weight) mutagen EMS aqueous solution for 23 hours to produce mutant seeds.
[0044] After EMS treatment, rinse the M thoroughly with water 1 Seeds, drained before planting in shallow water by sowing, and drained after 24 hours in shallow water. Three days later, the farmland was flooded again, and the farmland was kept flooded until it was drained for harvesting. Harvest M 2 The seeds are stored for the winter and screened for herbicide resistance in the spring of the following year. About 600 million grains of M are sown by sowing and shallow soil mixing 2 After seeding, half the field was sprayed with imazapic (trade name Cardre TM ), implement pre-emergent application, implement imazapyr (trade name Arsenal TM ) post-emergence application. 23 M. surviving after herbicide application were ...
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