Automatic correction method for ª€*N and ª€*C value of biological and soil sample

A soil sample and automatic correction technology, applied in the field of biology, can solve the problems of time-consuming data analysis and difficulty in judging the reliability and authenticity of the results.

Inactive Publication Date: 2004-03-17
NANJING UNIV
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

At present, this method only recognizes the data output format of Real 32 control software, and it is necessary to manually adjust the relevant parameters of the regression equation
In addition, using this method for data analysis is not only time-consuming, but also the same data can get different debugging results, making it difficult to judge the reliability and authenticity of the results
[0005] At present, there is no research on δ in biological and soil samples at home and abroad. 15 N and δ 13 Related reports on automatic correction of C value

Method used

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  • Automatic correction method for ª€*N and ª€*C value of biological and soil sample
  • Automatic correction method for ª€*N and ª€*C value of biological and soil sample
  • Automatic correction method for ª€*N and ª€*C value of biological and soil sample

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Experimental program
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Embodiment Construction

[0011] The present invention provides that the whole analysis process realized by computer program is as follows:

[0012] Perform data format conversion on data in Real 32 format;

[0013] Automatic detection of raw data (Isodat NT format), statistical standard reference, standard reference and sample δ 15 N and δ 13 The value of C and the number of corresponding peaks, report the statistical results, and give specific information on the erroneous data.

[0014] Calculation of standard substance δ 15 N and δ 13 C value and the area (or reciprocal) regression equation of its corresponding peak, combined with the principle of minimizing the deviation to screen the relevant data, and perform cyclic calculations to give the optimal regression equation, and then calculate the standard reference and sample δ 15 N and δ 13 Corrected values ​​for C and percentages of nitrogen and carbon, C / N and C / N molar ratios.

[0015] Example analysis results:

[0016] The example data com...

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Abstract

The present invention can realize the automatic calibration for the EA-IRMS system measured value in the biological and soil sample, wherein the optical regression equation of the value of the test norm substance for each test are offered, thus minimizing the deviation between the standard reference value and the true value, the real value and other relative parameter e.g. C / N, C%, N% of each example can be computed according to the optimal regression equation. The invention can be used to perform the automatic analysis for all the parameters above by means of the computer program, thus greatly reducing the calculation workload and possible errors that might may caused by the anthropogenic factors.

Description

1. Technical field [0001] This technique belongs to the field of biology. Especially concerning δ in biological and soil samples 15 N and δ 13 Automatic correction of C value, this method is also applicable to automatic correction of other isotope δ values. 2. Background technology [0002] Carbon and nitrogen stable isotopes are widely used in the field of ecosystem research, mainly used in the study of carbon and nitrogen sources, energy flow, nutrient structure, biomagnification of pollutants and system stability changes in ecosystems, and can be applied in Red tide research, environmental pollution control, ecological dynamics modeling, etc. (Cai Deling et al. 2002). In geochemical and ecological research, since the content of heavy isotopes of stable isotopes in nature is extremely low compared with light isotopes, it is internationally recognized that relative quantities are used instead of absolute quantities to represent the degree of accumulation of isotopes, usu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00
Inventor 何祯祥钦佩尹增芳王光周文宗
Owner NANJING UNIV
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