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Preparation for biological prevention and cure of hot pepper epidemic disease and method for producing the same

A technology for capsicum blight and capsicum phytophthora, applied in biocides, plant growth regulators, botanical equipment and methods, etc., can solve the problems of increasing pesticide residues in capsicum fruit, affecting the growth of capsicum, increasing production costs, etc.

Inactive Publication Date: 2004-11-10
NORTHWEST A & F UNIV +1
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AI Technical Summary

Problems solved by technology

The main problem of chemical control: Pepper blight is a soil-borne disease, and when chemical pesticides are used for control, pesticides must be applied to the soil
However, after pesticides are applied to the soil, a series of complex processes such as physics, chemical adsorption, migration, and microbial degradation and transformation will occur, which seriously affect the control effect of chemical pesticides; Residual amount, inhibit the growth of beneficial microorganisms in the soil, and will pollute the environment and increase production costs
Once the pepper blight is prevalent, it cannot be controlled with chemical pesticides
The chemical control research in the past 20 years has shown that the use of chemical pesticides alone to prevent and control pepper blight cannot fundamentally solve the problem. Under the current situation that there is no major progress in pepper disease-resistant breeding, it is very necessary to explore new breakthroughs

Method used

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Embodiment Construction

[0049] 1. Expanded cultivation of secondary strains: insert biocontrol bacteria 28G14 slant strains into 250ml soybean flour liquid medium [soluble starch 5.0, sucrose 10.0, peptone 2.0, yeast extract 2.0, NaCl 2.0, K 2 HPO 4 , MgSO 4 .7H 2 O 0.5, CaCO 3 1.0, Soybean Powder Soaking Juice (1L water plus 50g soybean powder, boil for 30 minutes, filter to get the juice) 1L, pH7.2~7.4, sterilize at 121°C for 30min. 〕In a 500ml Erlenmeyer flask, culture on a shaker at a constant temperature of 28°C for 3 days can be used as an inoculant for the preparation of bacterial agents.

[0050] 2. Bacterial agent preparation: mix ingredients according to No. 1 or No. 2 formula, then add Gaoshi No. 1 liquid medium according to the ratio of dry material: nutrient solution = 1: 0.30-0.45, stir evenly, and sterilize at 121°C for 30 minutes. Cool, inoculate the biocontrol bacteria suspension at a dosage of 5% to 10%, mix well, and culture it in a sterilized culture tray at 28°C for 4 to 5 da...

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Abstract

The invention relates to an microbiological viable bacteria preparation for prevention and cure of cayenne pepper fungus soil transferred diseases and process for preparation and use, wherein the active constituent of the preparation is actinomycete, which can produce antibiotics and can be fixed into the rhizosphere soil and root of the cayenne pepper, its mass propagation can repress the growth of Phytophthora capsici. The preparation is produced by solid state culturing method from farm and side line products as raw material. The major objective for prevention and cure of the invention is Phytophthora capsici.

Description

technical field [0001] The invention belongs to the technical field of biological control of crop diseases. The invention relates to a production method of live microbial preparations for preventing and treating pepper fungal soil-borne diseases. The main active ingredient of the capsicum blight biocontrol preparation is actinomycetes with specific functions. Background technique [0002] Pepper is a special agricultural product in my country, and it is also a vegetable and condiment that adds flavor and color in people's daily life, and it is also an important economic crop for exporting and earning foreign exchange. Pepper is planted in a wide range and a large area in my country. According to incomplete statistics, there are 27 provinces and regions in the country that have dried chili cultivation, with an annual planting area of ​​more than 5 million mu, a total annual output of 600,000 to 700,000 tons of dried chili, and an annual output value of about 5 billion yuan....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04C12N1/20
Inventor 薛泉宏蔡艳司美茹涂璇陈占全袁虎林来航线牛小磊
Owner NORTHWEST A & F UNIV
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