Small interference RNA molecule (siRNA) of attacking SARS virus mRNA melecule and application

A ribonucleic acid and small interference technology, applied in the field of genetic engineering, can solve the problems of high acute mortality and no effective and specific treatment methods

Inactive Publication Date: 2005-03-09
杭州新瑞佳生物医药技术开发有限公司
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Problems solved by technology

[0004] With the deciphering of the gene sequence of the SARS virus, people have successfully developed an effective method for accurately diagnosing SARS, but the biggest problem with SARS infection, like most other viral infections, is that there is no effective and specific treatment, which makes Its acute mortality rate remains high and has been around 5%-10%.

Method used

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  • Small interference RNA molecule (siRNA) of attacking SARS virus mRNA melecule and application
  • Small interference RNA molecule (siRNA) of attacking SARS virus mRNA melecule and application

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Embodiment 1

[2345] The preparation method of the small interfering RNA molecule (siRNA) provided by the present invention adopts the existing solid-phase chemical synthesis method. This method is available in: Wincott F, DiRenzo A, Shaffer C, Grimm S, Tracz D, Workman C, Sweedler D, Gonzalez C, Scaringe S and Usman N. Synthesis, degradation, analysis and purification of RNA and ribozymes. Nucleic AcidsRes. 1995, 23:2677-84.

[2346] Take the small interfering RNA molecule (SiRNA) of sequence number 2 of Table 1 as an example: the whole chemical synthesis can be roughly divided into four processes (1) synthesis of oligoribonucleic acid; (2) deprotection; (3) purification and separation; 4) Desalting and annealing aseptic disinfection.

[2347] Concrete preparation operation is as follows:

[2348] (1), synthesis of oligoribonucleic acid: the synthesis of siRNA is carried out on automatic DNA / RNA synthesizer (for example: Applied Biosystems EXPEDITE 8909), according to the nucleotide sequ...

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Abstract

A small-interference ribonucleic acid (SiRNA) molecule able to attack the mRNA molecular of SARS virus and its application in preparing the medicines for treating SARS and its virus infection associated diseases are disclosed. Said SiRNA is a double-stranded RNA molecule.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to a small interfering RNA molecule (SiRNA) attacking severe acute respiratory syndrome (SARS) virus mRNA molecule and application thereof. Background technique [0002] SARS is a new type of acute infectious disease with a history of only five months. The first SARS patient was discovered in Guangdong Province of my country in November 2002. The disease spread in Guangdong in February 2003. By mid-February 2003, the number of confirmed cases in Guangdong had reached 305, including 5 deaths. . At that time, because the clinical symptoms of this disease were similar to atypical pneumonia caused by other pathogens, it was also generally called atypical pneumonia (its original medical concept refers to not being caused by bacterial infection but other pathogens, mainly Lung inflammation caused by microorganisms such as chlamydia and mycoplasma). ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7088A61P11/00A61P31/14C07H21/02C12N15/50
Inventor 丁佳逸
Owner 杭州新瑞佳生物医药技术开发有限公司
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