Process for preparing optical pure (S)-2-octanol by microorganism and its special microorganism

A technology of microorganism method and octanol, applied in the field of biological separation of racemic compounds

Inactive Publication Date: 2005-03-23
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent CN1366550A has reported a kind of (R)-2-octanol dehydrogenase, which can preferentially oxidize (R)-2-octanol in the two optical isomers of 2-octanol, but it cannot Racemic 2-octanol is converted as a substrate to obtain (R)-2-octanol, a

Method used

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  • Process for preparing optical pure (S)-2-octanol by microorganism and its special microorganism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Cultivation of bacterial strains: The composition of the medium is that the components contained in each 100ml of culture solution are in grams: glucose 0.25-1.0, meat extract 0.5-2.0, peptone 0.5-2.0, yeast extract 0.1-0.5, (NH 4 ) 2 HPO 4 0.5~1.0, KH 2 PO 4 0.25~0.5, MgSO 4 ·7H 2 O 0.025~0.1, NaCl 0.001~0.005, ZnSO 4 ·7H 2 O 0.001~0.005, FeSO 4 ·7H 2 O0.001~0.005, CuSO 4 ·5H 2 O 0.0001~0.0005, MnSO 4 4H 2 O0.0001~0.0005.

[0057] The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 30%, the culture temperature is 25° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 72 hours.

Embodiment 2

[0059] Cultivation of the bacterial strain: the composition of the culture medium is the same as in Example 1. The culture conditions are as follows: the initial pH is 6.0-8.0, the filling volume is 5%, the culture temperature is 35° C., the rotation speed of the shaking flask is 100-300 rpm, and the culture time is 24 hours.

Embodiment 3

[0061] Preparation of whole cells: Take a loop of P.maltophilia CCTCC M204042 from the slope and inoculate it in a 250ml shaker flask with 30% culture solution at 25°C and 100-300rpm for 72 hours; The body was centrifuged and washed twice with saline, and the whole cells of the strain were collected for transformation reaction.

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Abstract

The invention is a method for preparing optically pure (S)-2-n-octyl alcohol by microbial method and its special microbe, screening and obtaining bacterial strain Pseudomonas maltophiliaSYB-4, CTCC M204042.It uses the bacterial strain to make cultivation and full-cell preparation, uses racemic2-n-octyl alcohol as substrate to make catalytic conversion reaction on microbe cell, and obtain a product (S)-2-n-octyl alcohol by nonsymmetrical conversion of the racemic2-n-octyl alcohol, where the optical purity of the product reaches above 80%e.e. The invention determines a reacting system and reacting conditions of full-cell nonsymmetrical conversion of racemic2-n-octyl alcohol for the bacterial strain, increasing the optical purity of the product.

Description

technical field [0001] The invention discloses a method for preparing optically pure (S)-2-octanol by a microbial method and a special microorganism thereof, which belong to the technical field of separating racemic compounds by a biological method. Background technique [0002] The chemical structure of 2-octanol is: [0003] [0004] Racemic 2-octanol consists of two enantiomers (R)-2-octanol and (S)-2-octanol. [0005] Optically pure 2-octanol, including (R)-2-octanol and (S)-2-octanol, is not only an anabolic steroid, vitamin E, and insect pheromones (bioinsecticides) but also many optically active drugs and It is an important chiral intermediate of pesticides, and is an indispensable and important chiral raw material for the preparation of high-performance liquid crystals and their devices. Its optical activity and high dielectric constant are closely related to many properties of liquid crystals; as an important intermediate, it is also Used in organic synthesis o...

Claims

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Application Information

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IPC IPC(8): C12P7/04
Inventor 徐岩聂尧穆晓清
Owner JIANGNAN UNIV
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