Double light beam detection modulation device based on surface plasma wave sensing

A surface plasmon wave and double beam technology, applied in the fields of photoelectric detection and biosensing technology, can solve the problem of inconvenient on-site online monitoring, and achieve the effect of easy design, enhanced operability, and avoidance of test errors.

Inactive Publication Date: 2006-01-04
ZHEJIANG UNIV
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Problems solved by technology

Although the angle adjustment method has high detection accuracy, the surface plasmon resonance angle modulation method requires a high-precision angle adjustment device, which is not convenient for on-site online monitoring, etc.

Method used

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  • Double light beam detection modulation device based on surface plasma wave sensing
  • Double light beam detection modulation device based on surface plasma wave sensing

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Embodiment Construction

[0013] As shown in the figure, the dual-beam detection and modulation device based on surface plasmon wave sensing has a laser (laser diode) 1, which is divided into a first beam 4 by optical components (including beam collimator, polarizer and beam splitter) 3 1. The second light beam 5 and two beams of polarized light are incident on the prism 2, and an Au film 8 is evaporated on the end surface of the prism 2, and a biosensitive film 9 is grown on the Au film, and the biosensitive film 9 is provided with a flow cell for the sample to be measured 10. Total reflection occurs at the end face of the prism 2, and the two beams of reflected light enter the first PD detector 6 and the second PD detector 7 respectively. Au film 8 has a thickness of 40 to 60 nm. Prism 2 is an isosceles triangular prism of K9 glass.

[0014] Au film 8 of 40-60nm is vapor-deposited on the end face of the prism by magnetron sputtering, and a layer of biomass-sensitive film 9 is grown on the Au film, s...

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Abstract

The double light beam detection and modulation device based on surface plasma wave sensing has laser, optical assembly to split the laser beam into the first polarized light beam and the second polarized light beam, prism with vaporization coated Au film, grown biosensitive film and pond for the measured sample to flow in to total reflect the incident two light beams, and the first PD detector and the second PD detector to receive these two reflected light beams separately. The present invention has the characteristic of surface plasma resonance (SPR) sensing technology maintained, and the double light beam SPR sensors, which need no precise scanning incident angle and thus simplified angle regulating mechanism, less measurement error and increased operation performance. The detection of double light beam light strength signal may be completed with cheap photodiode, and this can simplify the system, lower the manufacture cost and make the instrument portable.

Description

technical field [0001] The invention relates to biosensing technology and photoelectric detection technology, in particular to a dual-beam detection and modulation device based on surface plasma wave sensing. Background technique [0002] Generally, there are four detection and modulation methods for surface plasmon resonance (SPR): [0003] 1. Angle modulation Incidence of light with a fixed wavelength λ, change the incident angle θ of the incident light wave, under a certain measured refractive index, according to the equation ϵ pr ω c sin θ = ϵ ~ 1 ϵ ~ 2 ϵ ~ ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/41G01N21/17
Inventor 胡建东王晓萍袁克皋
Owner ZHEJIANG UNIV
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