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Process for reproducing virus of large brill hemorrhagic septicaemia by large brill fin clone

A technology for hemorrhagic sepsis and turbot, which is applied in the directions of viruses/phages, biochemical equipment and methods, microorganisms, etc., can solve problems such as failure to retrieve the successful reproduction of turbot virus.

Inactive Publication Date: 2006-06-28
OCEAN UNIV OF CHINA
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AI Technical Summary

Problems solved by technology

However, no report has been found on the successful mass reproduction of turbot virus, especially turbot hemorrhagic septicemia virus, the main pathogenic virus of turbot.

Method used

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Examples

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Embodiment Construction

[0008] As an example, the present invention provides detailed and specific preparation or propagation methods.

[0009] 1. Isolation and preparation of turbot hemorrhagic septicemia virus: 2 diseased fish with viral hemorrhagic leukemia collected from the turbot farm, cut off the viscera (liver, spleen and stomach, etc.) of the diseased fish with dissecting scissors ) about 20 grams, put it into a 50 ml glass homogenizer, add 20 ml of serum-free commercially available MEM culture medium, and perform homogenization treatment to break the cells. After the homogenate becomes a uniform paste, collect the homogenate and put it into In a 50 ml centrifuge tube, centrifuge at 15000 rpm for 90 minutes, collect the centrifuged supernatant, and filter it with a 0.45 μm microporous filter in a sterile room to remove bacteria and directly use it for cell inoculation; the excess should not be used immediately After the inoculated virus liquid is sealed, store it in a refrigerator at 4°C for...

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Abstract

The invention relates to the method used turbot fin cell line to breed turbot haemorrhagic septicemia virus. It includes the following steps: adopting turbot fin cell line cells as breeding system; inoculating the virus to the cell line cells; adsorbing at 22 centigrade degree for 2h; adding into 10% calf serum MEM culture solution to culture; gaining culture after inoculated 4 days; collecting turbot haemorrhagic septicemia virus liquid after once freeze thawing at -30 centigrade degree. The bred virus has large amounts and strong infection capability, and can be directly applied to viral vaccine manufacturing and production to improve turbot survival rate.

Description

technical field [0001] The invention relates to a method for propagating turbot hemorrhagic septicemia virus by using turbot fin cell line. Background technique [0002] Turbot aquaculture is an important pillar industry of marine aquaculture in my country, but the widespread spread of turbot infectious virus disease has brought a huge blow to the turbot aquaculture industry, resulting in heavy economic losses. How to solve the problem of prevention and treatment of turbot virus disease has become an important problem that urgently needs to be solved in the turbot aquaculture industry in the world today. Scholars believe that the use of virus vaccines to immunize turbot is an important way to prevent turbot virus diseases and improve the economic benefits of turbot aquaculture. In mammals and birds, there have been successful precedents of large-scale propagation of viruses and production of virus vaccines using cell lines, but so far, no reports have been reported in marin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12N7/02
Inventor 樊廷俊丛日山王丽燕耿晓芬杨秀霞李明玉于秋涛孙爱
Owner OCEAN UNIV OF CHINA
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