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High performance method for transgene tomato

A technology of gene transformation and tomato, applied in the field of agricultural biology, can solve the problems of low post-harvest value of agricultural products, low storage and processing volume, and intolerant varieties of storage.

Inactive Publication Date: 2010-06-09
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The post-harvest loss of my country's agricultural products is very alarming (the loss rate is 20%). The main reason is that the varieties are not resistant to storage, and the storage and processing volume is low, which directly leads to the low post-harvest value of my country's agricultural products.

Method used

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  • High performance method for transgene tomato
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Embodiment Construction

[0028] Embodiments of the present invention are described in further detail below:

[0029] Transformation method of the present invention is made of the following steps:

[0030] (1) Tomato seeds are sterilized and sowed on MS medium for cultivation until two cotyledons grow up.

[0031] First use sodium hypochlorite solution containing chloride ion concentration of 2.8 to sterilize tomato seeds for 10-20 minutes, then rinse three times with sterile water, inoculate on MS medium for cultivation, the cultivation time is 1-3 weeks, and the environmental conditions for cultivation are: The temperature is 25-28°C, the light is about 12 hours a day, and the light intensity is 2500-5000LUX.

[0032] (2) Preparation of Agrobacterium liquid for transformation

[0033] Take a single colony of Agrobacterium carrying the tomato antisense ethylene gene and inoculate it on YEB liquid medium, shake at 200 rpm for 48 hours at 25-28°C, add 50mg / L of kana to the liquid medium Mycin and 100...

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Abstract

An efficient tomato gene transform method for obtaining a kind of antisanility tomato with long storage period features use of the Agrobacterium transgenic engineering to clone and antisensely configure ether receptor protein gene for transforming tomato. Said method includes such steps as pre-treating with the bleeding sap of potato, treating with auxin, screening transformed bud, and elongationculture.

Description

[0001] Field [0002] The invention belongs to the field of agricultural biotechnology or the field of plant genetic engineering, in particular to a high-efficiency tomato gene transformation method. Background technique [0003] The breeding of transgenic anti-aging and storage-resistant tomato varieties is a joint research and development project of Tianjin Agricultural University and China Agricultural University. Funded by the National Natural Science Foundation of China since 1995, new tomato varieties T95-1, T96-2, and T98-3 with antisense ACS and ACC genes for storage have been obtained through gene silencing technology. In 2001, ethylene receptors were also funded by the Tianjin Education Commission. The protein gene-antisense NR gene was introduced into tomato to obtain anti-aging and storage-resistant new variety N2, and successively obtained new varieties F1, F2, and F3 with antisense CTR1, ENT1 and EIN2 genes. At present, we have obtained 7 varieties and 28 types;...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/00C12N15/63C12N5/04C12N15/82
Inventor 杨静慧刘艳军罗云波张伟玉杨恩芹
Owner TIANJIN AGRICULTURE COLLEGE