Cell Culture Tool

a cell culture and tool technology, applied in the field of tissue culture vessels, can solve the problems of high difficulty and tedious evaluation of individual cell types after being physically mixed or by using membranes, and the inability of the vitro system to accurately predict in vivo effects, and achieve the effect of not reducing the harmful effect of agents

Inactive Publication Date: 2007-07-26
DISCOVERY LIFE SCI LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

Facilitates the evaluation of drug metabolism and toxicity across multiple cell types, simulating in vivo conditions by allowing cells from different organs to share a common medium, enabling the determination of therapeutic indices and pharmacological effects while maintaining the ability to analyze individual cell types separately.

Problems solved by technology

However, it is also recognized that an in vitro system may not accurately predict in vivo effects due to the lack of the complexity and interplay of biological processes for in vitro systems.
However, in vivo, the substance may be metabolized by other organs such as the kidneys, and the resulting metabolite may have a different effect on liver cells which would not be detected by using liver cells alone.
Evaluation of the individual cell types after being physically mixed or by using membranes can be highly difficult and tedious.

Method used

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  • Cell Culture Tool
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Examples

Experimental program
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Embodiment Construction

[0055] Implementations of the tool 200, 300, 400 embodying the current invention are shown in FIGS. 2A-5B and FIG. 10. The tool 200, 300, 400 includes multiple wells within each a type of cells can be cultured, but each well can be overfilled or flooded, so that the cells in the different wells can share a common medium. This is achieved by configuring each well as an indentation inside a larger plate (FIGS. 2A and 2B), placing short partitions inside a larger plate (FIGS. 3A and 3B), or placing small inserts inside a larger plate (FIGS. 4A and 4B). However, this invention can be applied to any multi-well format with any number of wells per plate.

[0056] Referring to FIGS. 2A and 2B, a multi-well tool 200 of the present invention comprises a body 205 having a substantially planar top surface 210, and an outer wall 215 extending from the body 205. Six wells 220 are formed in the body 205 by depressions in the top surface 210. Each well 220 has a containing wall 225 that may slant dow...

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Abstract

A cell culture tool includes a cell culture tray and a dish or insert tray that rests on the cell culture tray. The cell culture tray includes a substantially flat surface with more than one well, each well configured to contain a liquid volume and an outer wall surrounding the tray to define a reservoir configured to fluidly interconnect the volume of the more than one well. The insert dish includes a permeable membrane and the dish is configured to rest on the cell culture tray to position the membrane within the reservoir.

Description

CROSS REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 10 / 751,983 filed on Jan. 7, 2004, and claims priority to U.S. Provisional Application No. 60 / 754,327filed on Dec. 29, 2005, and U.S. Provisional Application No. 60 / 518,331 filed Nov. 10, 2003, which are incorporated herein by reference.FIELD OF THE INVENTION [0002] This invention relates to tissue culture vessels and, more particularly, comprises a new and improved tissue culture vessel for co-culturing of multiple types of cells and tissues and the application of the cell culture device in biological studies, including application in drug discovery and development. BACKGROUND OF THE INVENTION [0003] Culture tools, such as, for example, dishes, plates, flasks, and other types of vessels, are widely used in the laboratory for a variety of purposes. In normal use, cell and tissue culturing involves the use of agar or medium, which covers the bottom of a well. Cells are...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12M1/34C12M1/12A01N1/02C12M1/00C12M1/20C12M1/24C12M3/00C12M3/04C12NC12N5/00
CPCC12M35/08C12M23/12C12M25/04C12N5/0662
InventorLI, ALBERT
OwnerDISCOVERY LIFE SCI LLC