Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cultivation of Primate Embryonic Cells

a technology of primate embryonic cells and embryonic cells, which is applied in the field of primate embryonic cell culture to achieve the effects of avoiding the variability of results due to differences in animal serum batches, avoiding the use of animal serum, and increasing the efficiency of cloning

Inactive Publication Date: 2009-01-22
THOMSON JAMES A +1
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0019]The culturing permits the embryonic stem cells to stably proliferate in culture for over one month (preferably over six months; even more preferably over twelve months) while maintaining the potential of the stem cells to differentiate into derivatives of endoderm, mesoderm, and ectoderm tissues, and while maintaining the karyotype of the stem cells.
[0023]Variability in results due to differences in batches of animal serum is thereby avoided. Further, it has been discovered that avoiding use of animal serum while using fibroblast growth factor can increase the efficiency of cloning.

Problems solved by technology

However, when one or more well defined purified component(s) of such serum is added, they do not.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

examples

[0033]In the first experiments described here human ES cells were plated on irradiated (35 gray gamma irradiation) mouse embryonic fibroblasts. Culture medium for the present work consisted of 80% KNOCKOUT™ Dulbeco's modified Eagle's medium (DMEM) (Gibco BRL, Rockville, Md.), 1 mM L-Glutamine, 0.1 mM β-mercaptoethanol, and 1% nonessential amino acids stock (Gibco BRL, Rockville, Md.), supplemented with either 20% fetal bovine serum (HyClone, Logan, Utah) or 20% KNOCKOUT™ serum replacement (SR), a serum-free replacement originally optimized for mouse ES cells (Gibco BRL, Rockville, Md.). The components of KNOCKOUT™ SR are those described for serum replacements in WO 98 / 30679.

[0034]In alternative experiments medium was supplemented with either serum or the aforesaid serum replacer KNOCKOUT™ SR, and either with or without human recombinant basic fibroblast growth factor (bFGF, 4 ng / ml). The preferred concentration range of bFGF in the culture was between 11 ng / ml to 500 ng / ml.

[0035]To ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to methods for culturing human embryonic stem cells by culturing the stem cells in an environment essentially free of mammalian fetal serum and in a stem cell culture medium including amino acids, vitamins, salts, minerals, transferring, insulin, albumin, and a fibroblast growth factor that is supplied from a source other than just a feeder layer the medium. Also disclosed are compositions capable of supporting the culture and proliferation of human embryonic stem cells without the need for feeder cells or for exposure of the medium to feeder cells.

Description

CROSS REFERENCES TO RELATED APPLICATIONS[0001]This application is a continuation-in-part of application of U.S. patent application Ser. No. 10 / 952,096, which is a continuation-in-part application of U.S. patent application Ser. No. 09 / 522,030 filed Mar. 9, 2000.STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH[0002]To be determined.BACKGROUND OF THE INVENTION[0003]The present invention relates to methods for culturing primate embryonic stem cell cultures and culture media useful therewith.[0004]Primate (e.g. monkey and human) pluripotent embryonic stem cells have been derived from preimplantation embryos. See, for example, U.S. Pat. No. 5,843,780 and J. Thomson et al., 282 Science 1145-1147 (1998). The disclosure of these publications and of all other publications referred to herein are incorporated by reference as if fully set forth herein. Notwithstanding prolonged culture, these cells stably maintain a developmental potential to form advanced derivatives of all three embryonic ger...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/06C12N5/0735
CPCC12N5/0606C12N2500/25C12N2501/115C12N2500/99C12N2500/44C12N2500/90C12N5/06C12N5/00
Inventor THOMSON, JAMES A.LEVENSTEIN, MARK
Owner THOMSON JAMES A
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com