Apparatus and method for separating and refining product manufactured by microbial fermentation by using adsorbent
a technology of microbial fermentation and adsorbent, which is applied in the field of apparatus and a method for continuously separating and refining products produced by microbial fermentation, can solve the problems of inefficient production of biobutanol, low production amount of butanol in a culture medium, and complex method for preparing butanol based on petroleum, etc., to achieve simple and rapid separation and purification
Inactive Publication Date: 2014-02-06
GS CALTEX CORP
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Benefits of technology
The fermentation, separation, and refinement apparatus and method described in this patent make it easy and fast to separate and purify products from the culture medium of a microbe.
Problems solved by technology
However, since the method for preparing butanol based on petroleum is complicated due to using high temperature and high pressure and large amounts of hazardous wastes and carbon dioxide are discharged (Document [Tsuchida et al., Ind. Eng. Chem. Res., 45: 8634, 2006]).
An interest in research into biobutanol has been rapidly increased around the world due to an increase in oil price, environmental problems, and the like, but biobutanol has not yet been efficiently produced.
However, even in the case of using these recombinant strains, the production amount of butanol in a culture medium was significantly low (20 g / L or less) due to toxicity of butanol, which is a final product, such that it was impossible to industrially use these recombinant strains.
However, in this method, only butanol is selectively adsorbed by the activated carbon and a concentration of the adsorbed butanol is low, such that it is difficult to recover butanol, and physical stability of activated carbon is insufficient, such that it is impossible to reuse the activated carbon.
Therefore, there is a disadvantage in that extraction of butanol is expensive.
An adsorption amount of butanol is in proportion to a concentration of butanol, but the concentration of butanol produced in continuous culture is low, such that the adsorption amount of butanol is also significantly low.
In addition, a column filled with the activated carbon may be physically clogged due to aggregation of cells, thereby causing a problem in a process.
In this method, cell aggregates may clog the column and form a channel in a flow of the culture medium, such that it is difficult to allow the products such as butanol, acetone, isopropanol, ethanol, or the like, to be adsorbed in the entire adsorbent, thereby decreasing adsorption efficiency.
In addition, impurities produced by microbes in the culture process and sugar, which is a raw material, are simultaneously adsorbed, such that purity and productivity of butanol are low at the time of recovering butanol.
Further, an adsorption amount of butanol is in proportion to an amount of adsorbent added to the culture medium, but in this method, there is a limitation in an addition amount of the adsorbent in the culture medium.
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[0084]100: fermentation, separation, and refinement apparatus[0085]110: first column 120: second column[0086]112: adsorbent 114: filter[0087]130: culture bath 140: supply bath[0088]150: storage bath 160: pump[0089]170: first conversion part 172: second conversion part[0090]174: third conversion part 176: fourth conversion part
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Abstract
The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency.
Description
TECHNICAL FIELD[0001]The present invention relates to an apparatus and a method for continuously separating and refining products produced by microbial fermentation.BACKGROUND ART[0002]Butanol can be used as a chemical intermediate in cosmetics, perfume, hormone, a sanitizer, an industrial coating agent, a paint additive, fiber, a plastic monomer, medical supplies, vitamins, antibiotics, pesticides, or the like (Document [Durre, Biotechnol. J, 2:1525-1534, 2007]).[0003]As the existing method for preparing butanol, a method of fermenting sugar using Clostridium strain to produce butanol, acetone, and ethanol (Document [US Patent Laid-Open Publication No. 1,315,585]) has been used until the 1980s, but thereafter, an oxo process of synthesizing butanol from propylene obtained from petroleum has been widely used. However, since the method for preparing butanol based on petroleum is complicated due to using high temperature and high pressure and large amounts of hazardous wastes and carb...
Claims
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IPC IPC(8): C12M1/00C12P7/28C12P7/06C12P7/16
CPCC12M47/10C12P7/06C12P7/28C12P7/16C12M21/12C12M23/58C12M25/18C12M25/20C12M47/12B01D15/08B01D15/1885Y02E50/10C12P7/065C12M1/06B01D15/22C12P7/04
InventorLEE, SANG-HYUNEOM, MOON-HOLEE, JULIAJEON, SANG-JUNCHO, JUNG-HEECHOI, JIN DAL RAE
OwnerGS CALTEX CORP