Calcium-Mediated Effects of Coral and Methods of Use Thereof
a coral and calcium-mediated technology, applied in the field of coral scaffolds, can solve the problems of stress shielding to the surrounding bone, fatigue failure of the implant, limited autologous bone tissue, etc., and achieve the effects of prolonging the remission of cancer, preventing a relapse of cancer, and reducing the incidence of cancer
Inactive Publication Date: 2015-12-17
BEN GURION UNIVERSITY OF THE NEGEV
View PDF5 Cites 4 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Benefits of technology
The present invention provides a method for inducing bone and cartilage formation in a subject by administering a composition containing coral that is seeded with a precursor cell in the presence of a chelator. The invention also provides a method for treating cancer or inhibiting cancer progression by contacting a neoplastic or pre-neoplastic cell in a subject with a composition containing coral that upregulates cadherin levels. The invention also provides a method for preventing a relapse of cancer or reducing the incidence of cancer by treating a neoplastic, pre-neoplastic, or hyperplastic cell in a subject with a composition containing coral that upregulates cadherin levels. The invention also provides a method for in vivo calcium release by contacting cells in a subject with an aragonite or calcite-producing species. The technical effects of this invention include promoting bone and cartilage formation, treating cancer, inhibiting cancer progression, and inducing calcium release for bone and cartilage formation.
Problems solved by technology
Synthetic materials such as metals and bone cements have also been used for many years, but often result in stress-shielding to the surrounding bone and fatigue failure of the implant.
Another possibility is autologous bone grafting, although the supply of autologous bone tissue is limited and its collection is painful, with the risk of infection, hemorrhage, cosmetic disability, nerve damage, and loss of bone function.
In addition, significant morbidity is associated with autograft harvest sites.
However, these scaffolds often are mechanically unstable and do not readily contribute to the creation of tissue structures with a specific predefined shape for transplantation.
While most candidate materials must be synthetically modified to meet the essential requirements of an adequate bone and / or cartilage substitute, this is not necessarily the case for Marine skeletal material, such as natural coral exoskeletons.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
example 1
Growth of Mesenchymal Stem Cells Grown on Porites Lutea
[0158]In order to determine calcium-mediated effects of coral on mesenchymal stem cell (MSC) growth, Scanning Electron Micrographs (SEMs) were taken of coral seeded with MSCs with and without incubation with a calcium chelator. SEMs showed increasing MSC density in both porites lutea (POR) and gold-coated POR (GCPOR) biomatrices at 1, 4, and 7 days post seeding (FIGS. 2A and 2B). Incubation with the calcium chelator, EGTA further increased local cell density (FIGS. 2Aj, 2Al).
example 2
Calcium Uptake of Mesenchymal Stem Cells Grown on Porites Lutea
[0159]Calcein labeling of MSCs seeded on biolabeled biomatrices showed calcium uptake from the biomatrix at 7 days post-seeding (FIGS. 3B-D). There was a greater increase in labeled calcium uptake within MSCs at 10 days post-seeding as compared to 3 days post-seeding in control and EGTA-treated matrices (FIG. 3E).
example 3
Cadherin Changes in Mesenchymal Stem Cells Grown on Porites Lutea
[0160]Cadherin-11 (Cad-11) and N-cadherin (N-Cad) mRNA levels in MSCs increased as a function of days post-seeding, with Cad-11 and N-Cad peak expression at 21 days in non-gold-coated and 14 days in gold-coated porites lutea. Cad-11 expression was higher in non-gold-coated porites lutea, while N-Cad expression was higher in gold-coated porites lutea (FIGS. 4A-B).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
This invention is directed to coral scaffolds seeded with precursor cells in culture in the presence of a chelator and uses thereof in inducing or enhancing bone and / or cartilage formation in a subject, and kits related thereto. This invention is also directed to use of cadherin-upregulating coral for treating cancer or inhibiting cancer progression. This invention is also directed to use of aragonite or calcite-producing species for in vivo calcium release, and its application to the treatment of skin diseases, disorders or conditions.
Description
FIELD OF THE INVENTION[0001]This invention is directed to coral scaffolds seeded with precursor cells in culture in the presence of a chelator and uses thereof in inducing or enhancing bone and / or cartilage formation in a subject, and kits related thereto. This invention is also directed to use of cadherin-upregulating coral for treating cancer or inhibiting cancer progression. This invention is also directed to use of aragonite or calcite-producing species for in vivo calcium release.BACKGROUND OF THE INVENTION[0002]Surgical intervention and grafting are sometimes necessary to restore mechanical function and reconstruct the morphology of bone resulting from trauma, tumors, or abnormal bone developments. Synthetic materials such as metals and bone cements have also been used for many years, but often result in stress-shielding to the surrounding bone and fatigue failure of the implant. Another possibility is autologous bone grafting, although the supply of autologous bone tissue is ...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61L27/36A61L27/54A61L27/38A61K35/28A61K35/614
CPCA61L27/3604A61L27/3847A61L27/3852A61L27/365A61L27/3654A61L27/3687A61L2300/412A61L27/3834A61L2430/40A61L2430/02A61L2430/06A61L2300/102A61L27/54A61K35/614A61K35/28A61P17/00A61P19/00A61P19/02A61P19/08A61P19/10A61P35/00
InventorVAGO, RAZI
OwnerBEN GURION UNIVERSITY OF THE NEGEV