Phosphate-solubilizing composite microorganism fungicide and application thereof

A compound microbial inoculant and phosphorus-dissolving technology, applied in microorganisms, biocides, microorganism-based methods, etc., can solve the shortage of phosphorus-dissolving biological strain resources, the research and development of phosphorus-dissolving microbial inoculants has not been reported, and the world has not. See reports and other issues to achieve the effect of efficiently dissolving insoluble phosphorus, preventing and controlling various diseases and increasing crop yield.

CN102433280BInactive Publication Date: 2013-07-31INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2013-07-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a phosphate-solubilizing composite microorganism fungicide, which has effective components of pantoea ananatis, pythium oligandrum, trichoderma harzianum and paecilomyceslilacinus. According to the invention, high-efficiency phosphate-solubilizing bacterium-increase bacterium pantoea ananatis with wide applications and fungus of high-efficiency antagonistic crop disease areorganically combined, by applying the composite fungicide of the invention, while soil phosphorin is efficiently utilized and phosphatic fertilizer utilization rate is improved, the effect of antagonistic microorganisms in preventing soil-borne diseases can be sufficiently played, and the effects of solubilizing phosphate to increase yield and resisting diseases to increase yield are achieved. The phosphate-solubilizing composite microorganism fungicide has tremendous application value.
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Description

technical field

[0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a phosphorus-dissolving composite microbial bacterial agent and its application. Background technique

[0002] (1) Development Status of Phosphorus-Solubilizing Microbial Agents

[0003] More than 90% of the cultivated land in my country is deficient in phosphorus, and the use of phosphorus fertilizer is the main measure to solve the problem of crop yield increase. Phosphorus fertilizer has increasingly become a bottleneck restricting the sustainable development of agriculture in our country. my country is the world's largest consumer of chemical fertilizers and the largest consumer of phosphate fertilizers, with an annual consumption of about 11 million tons of phosphate fertilizers. As we all know, my country is short of phosphate rock resources, with reserves of 15.198 billion tons. In another 20 years, the medium and high-grade phosphate rock will be exh...

Examples

Embodiment 1

[0047] Example 1 Isolation and cultivation of Pantoea ananatis (Pantoea ananatis) P41-3A

[0048] Pantoea ananatis (Pantoea ananatis) P41-3A was isolated from the soil of Liuyang Phosphate Mining Area, Hunan Province, my country, and has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, address: No. 1 Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, date of deposit: December 12, 2011, deposit number CGMCC NO.5568.

[0049] Cultivation of Phosphorus Solubilizing and Promoting Pantoea pineapple P41-3A:

[0050] (1) Pantoea pineapple P41-3A medium

[0051] Fermentation broth formula (kg / t fermentation broth): dipotassium hydrogen phosphate 0.1, potassium dihydrogen phosphate 0.1, sodium chloride 4.3, magnesium sulfate 0.1, starch 8.6, beef extract 4.3, peptone 4.3, yeast powder 4.3, sucrose 8.6, sulfuric acid Ammonium 2.6, calcium carbonate 10, ferrous sul...

Embodiment 2

[0057] The preparation of embodiment 2 high-efficiency phosphate-dissolving disease-resistant disease-increasing microbial bacterial agents

[0058] 1. Cultivation of Pythium oligandrum, Trichoderma harzianum and Paecilomyces lilacinus

[0059] Pythium oligandrum (Pythium oligandrum) is preserved in the Czech National Culture Collection Center (CCF), with the preservation number CCF1417; Paecilomyces lilacinus is deposited with the American Collection of Type Microorganisms (ATCC) under the accession number ATCC38740.

[0060] (1) Composition of medium (kg / t fermentation liquid)

[0061] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH 7.0, prepared with water.

[0062] (2) Fermentation and cultivation process

[0063] Pythium oligandrum, Trichoderma harzianum and Paecilomyces lila...

experiment example 1

[0068] The effect of dissolving insoluble phosphorus under the condition of experimental example 1 Pantoea pineapple P41-3A culture dish

[0069] 1. From the acidic soil samples in Liuyang Phosphate Mine Area, Hunan Province, China, the phosphate-dissolving bacteria Pantoea ananatis P41-3A was isolated and screened. At the same time, the phosphorus-dissolving bacteria strains P40, P36 and P40-2 were isolated and screened. Under the conditions of indoor solid culture dish and liquid culture medium, their phosphorus-dissolving ability was determined.

[0070] The ratio of the phosphorus-dissolving circle to the colony diameter of the phosphorus-dissolving strain P41-3A reached 4.80, and the water-soluble soluble (P 2 o 5 ) was 3.76mg; the phosphorus-dissolving circle and the colony diameter ratio of bacterial strains P40, P36 and P40-2 were 4.00, 2.83 and 1.56 respectively, and the phosphorus-dissolving amount in the phosphorus-dissolving circle (P 2 o 5 ) were 3.13, 2.21 and...