Compound with anti-aging function and application thereof

Abstract

The invention discloses a compound with an anti-aging function. The compound comprises heteropanax fragrans total triterpenoids, the heteropanax fragrans total triterpenoids is composed of heteropanaxfragrans triterpenoid saponins and heteropanax fragrans triterpene acid, and the mass ratio of the heteropanax fragrans triterpenoid saponins to the heteropanax fragrans triterpene acid is (1-5):(1-8). According to the provided compound with the anti-aging function, synergetic compounding of the heteropanax fragrans triterpenoid saponins and the heteropanax fragrans triterpene acid in the heteropanax fragrans total triterpenoids is achieved, compared with the single using of the triterpenoid saponins or the triterpene acid, the synergetic compounding of the heteropanax fragrans triterpenoid saponins and the heteropanax fragrans triterpene acid promoting the synthesis of collagen can be more effectively improved, collagenous fibers can be increased, the skin becomes thick, and the skin metabolic function is improved to delay the skin aging.

Description

technical field

[0001] The invention relates to the field of cosmetics, in particular to a compound with anti-aging function and its application. Background technique

[0002] [Heteropanax fragrans (Roxb.) Seem. ] is an evergreen tree belonging to Araliaceae (Araliaceae), which is mainly distributed in the coastal provinces of southern my country, and is often used as a garden ornamental plant. Its roots, stems and leaves can be used as medicine, which has the effects of cooling blood, detoxifying, reducing swelling and relieving pain. It has been reported in the literature that some phenolic acids, saponins and triterpenoids have been isolated from the rhizomes and branches of A. In recent years, the research on the activity of related extracts of Fructus japonica found that its active ingredients can be used as platelet aggregation inhibitors, antioxidants, histamine release inhibitors, active oxygen scavengers, etc. The use of saponins and triterpenoids in Fructus japo...

Examples

Embodiment 1

[0018] (1) Normal human skin fibroblasts were inoculated on cell culture plates at an appropriate density, digested with 0.25% trypsin, and then prepared into a single cell suspension with DMEM medium containing 10% fetal bovine serum by volume. solution, 1×10 per well 5 Inoculated in 96-well plates or cell culture dishes (30mm), adhered to the wall overnight, and discarded the supernatant. Then add DMEM medium without fetal bovine serum, starve for 24 hours, and synchronize the cells in each group, in 5% CO 2 , Cultured in a 37°C incubator.

[0019] The experiment was divided into untreated blank control group, and 3 treatment groups, respectively, carotin 50ug / ml in treatment group 1, carotin 25ug / ml in 2 and oleanolic acid 25ug / ml, carotin in treatment group 3 25ug / ml and 25ug / ml of melaleucaic acid, all three treatment groups were prepared with DMEM medium without fetal bovine serum. After cell starvation treatment, active ingredients were added for treatment, and a con...

Embodiment 2

[0022] Carry out the test according to the method for cell culture in the embodiment one, the experiment divides the untreated blank control group, and 5 treatment groups, are respectively the oleanolic acid 30ug / ml of the treatment group 1, the melaleuca acid of the treatment group 2 30ug / ml, 15ug / ml of oleanolic acid and 15ug / ml of oleanolic acid in treatment group 3, 15ug / ml of oleanolic acid and 15ug / ml of carrotin in treatment group 4, and 15ug / ml of oleanolic acid in treatment group 5 15ug / ml and carrotin 15ug / ml. All five treatment groups were formulated with DMEM medium without fetal bovine serum. After cell starvation treatment, active ingredients were added for treatment respectively, and a control group was set up, and 6 duplicate wells were set up respectively. After culturing for 48 hours, the supernatant was centrifuged, and the content of procollagen type I was determined according to the detection kit (ProcollagenTypeIC-Peptide (PIP) EIAKit) instructions, dete...

Embodiment 3

[0025] An anti-aging face cream comprises the following components in mass percentage: polyglyceryl-3 methylglucose distearate 1.5%, glyceryl stearate 0.1%, PEG-100 stearate 1%, palm Ethylhexyl 1%, caprylic / capric triglyceride 5%, oleyl erucate 0.1%, hydrogenated palm oil glyceride citrate 1%, avocado tree (BUTYROSPERMUM PARKII) fruit butter 1%, poly Dimethicone 3%, Cyclopentasiloxane 1%, Tocopheryl Acetate 1%, Allantoin 0.1%, Butylene Glycol 8%, Glycerin 1%, Methylparaben 0.3 %, Propylparaben 0.1%, Ammonium Acryloyldimethyltaurate / VP Copolymer 0.5%, Sodium Hyaluronate 0.01%, Carbomer 0.3%, Betaine 0.1%, Aminomethylpropanol 0.15% , manganese gluconate 0.01%, phenoxyethanol 0.3%, compound 0.2%, water balance, wherein the mass ratio of saponin and triterpene acid in the compound is 1:1.

technical field

[0001] The invention relates to the field of cosmetics, in particular to a compound with anti-aging function and its application. Background technique

[0002] [Heteropanax fragrans (Roxb.) Seem. ] is an evergreen tree belonging to Araliaceae (Araliaceae), which is mainly distributed in the coastal provinces of southern my country, and is often used as a garden ornamental plant. Its roots, stems and leaves can be used as medicine, which has the effects of cooling blood, detoxifying, reducing swelling and relieving pain. It has been reported in the literature that some phenolic acids, saponins and triterpenoids have been isolated from the rhizomes and branches of A. In recent years, the research on the activity of related extracts of Fructus japonica found that its active ingredients can be used as platelet aggregation inhibitors, antioxidants, histamine release inhibitors, active oxygen scavengers, etc. The use of saponins and triterpenoids in Fructus japo...

Examples

Embodiment 1

[0018] (1) Normal human skin fibroblasts were inoculated on cell culture plates at an appropriate density, digested with 0.25% trypsin, and then prepared into a single cell suspension with DMEM medium containing 10% fetal bovine serum by volume. solution, 1×10 per well 5 Inoculated in 96-well plates or cell culture dishes (30mm), adhered to the wall overnight, and discarded the supernatant. Then add DMEM medium without fetal bovine serum, starve for 24 hours, and synchronize the cells in each group, in 5% CO 2 , Cultured in a 37°C incubator.

[0019] The experiment was divided into untreated blank control group, and 3 treatment groups, respectively, carotin 50ug / ml in treatment group 1, carotin 25ug / ml in 2 and oleanolic acid 25ug / ml, carotin in treatment group 3 25ug / ml and 25ug / ml of melaleucaic acid, all three treatment groups were prepared with DMEM medium without fetal bovine serum. After cell starvation treatment, active ingredients were added for treatment, and a con...

Embodiment 2

[0022] Carry out the test according to the method for cell culture in the embodiment one, the experiment divides the untreated blank control group, and 5 treatment groups, are respectively the oleanolic acid 30ug / ml of the treatment group 1, the melaleuca acid of the treatment group 2 30ug / ml, 15ug / ml of oleanolic acid and 15ug / ml of oleanolic acid in treatment group 3, 15ug / ml of oleanolic acid and 15ug / ml of carrotin in treatment group 4, and 15ug / ml of oleanolic acid in treatment group 5 15ug / ml and carrotin 15ug / ml. All five treatment groups were formulated with DMEM medium without fetal bovine serum. After cell starvation treatment, active ingredients were added for treatment respectively, and a control group was set up, and 6 duplicate wells were set up respectively. After culturing for 48 hours, the supernatant was centrifuged, and the content of procollagen type I was determined according to the detection kit (ProcollagenTypeIC-Peptide (PIP) EIAKit) instructions, dete...

Embodiment 3

[0025] An anti-aging face cream comprises the following components in mass percentage: polyglyceryl-3 methylglucose distearate 1.5%, glyceryl stearate 0.1%, PEG-100 stearate 1%, palm Ethylhexyl 1%, caprylic / capric triglyceride 5%, oleyl erucate 0.1%, hydrogenated palm oil glyceride citrate 1%, avocado tree (BUTYROSPERMUM PARKII) fruit butter 1%, poly Dimethicone 3%, Cyclopentasiloxane 1%, Tocopheryl Acetate 1%, Allantoin 0.1%, Butylene Glycol 8%, Glycerin 1%, Methylparaben 0.3 %, Propylparaben 0.1%, Ammonium Acryloyldimethyltaurate / VP Copolymer 0.5%, Sodium Hyaluronate 0.01%, Carbomer 0.3%, Betaine 0.1%, Aminomethylpropanol 0.15% , manganese gluconate 0.01%, phenoxyethanol 0.3%, compound 0.2%, water balance, wherein the mass ratio of saponin and triterpene acid in the compound is 1:1.

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