A kind of high temperature-resistant Mucor racemosa and its application

Abstract

The invention discloses a high temperature-resistant Mucor racemosa and its application, belonging to the application field of microbial fermentation technology. The strain of the present invention is isolated from Mucoralpha koji produced in Sichuan. After separation and identification, it is determined to be Mucorus racemosa, named M-THF-02, and the preservation number is CCTCC NO: M 2020552; the preservation time is September 2020 14th. The optimum growth temperature of the mucormycetes obtained by the invention is 28-30° C., which overcomes the problem that the traditional mucormycetes are not resistant to high temperature. Applied to the production of mucor soy sauce, it has strong protease production ability, high acid protease activity, and short fermentation cycle. The bacteria grows rapidly and has long mycelium, which can effectively improve production efficiency. Stability and safety of product quality. And the present invention obtains mucormycete THF-02, compared with traditional mucormyces fermented soya bean koji with a thickness of 15-20cm, THF-02 not only significantly improves the thickness of koji to 30cm, but also is used for fermented soya bean sauce made during disc fermentation , Soybean aroma, strong fresh aroma, excellent color and mellow taste.

Description

technical field

[0001] The invention relates to a strain of high-temperature-resistant mucormycetes racemosus and its application, in particular to a strain of high-temperature-resistant mucormyces racemosus M-THF-02 and its application, and belongs to the field of microbial fermentation technology application. Background technique

[0002] Mucor ( Mucor ) is a common strain of fermented products such as fermented bean curd and tempeh. It can decompose soybean protein in soybean products, and has the ability to saccharify starch and degrade cellulose. Due to the long-term domestication of mucormycetes under high-protein environmental conditions, it has the ability to synthesize and secrete a variety of extracellular proteases. Enzyme system, pectinase system, etc. form a compound enzyme system to catalyze a variety of biochemical reactions, so that the fermentation product has unique nutritional and health functions.

[0003] The most commonly used mucormycetes in fermente...

Examples

Embodiment 1

[0041] Screening and isolation of thermotolerant Mucor racemosus M-THF-02

[0042] The purpose of the present invention is to isolate the strains of fermented soya bean koji produced by Chengdu Taihefang Brewing Co., Ltd., address: No. 388, Qingxiang Road, Xinfan Town, Xindu District, Chengdu City, Sichuan Province, production batch number: 20181210; isolate a raceme Mucor M-THF-02, and the isolated Mucor racemosa is made into a third-grade strain for application in fermented soybean meal, thereby shortening the fermentation cycle, improving its nutritional content and production efficiency, and ensuring the controllability of the product Sex, thereby reducing the risk of the product.

[0043] 1. Screening of strains

[0044]Add physiological saline one by one from 150 samples for dilution, and select a dilution gradient of 10 -2 、10 -3 Spread the sample dilution on a PDA plate and incubate at 28°C for 48-72 hours, pick and select vigorously growing colonies, and inoculate ...

Embodiment 2

[0078] The application of the industrialized production of Douchi of target Mucor racemosus M-THF-02

[0079] 1. Application method

[0080] (1) Insert Mucorus racemosa and THF-02 for production into PDA slant medium under sterile conditions and culture at 28°C for 48-72 hours, until the slant medium is covered with Mucoral spores (gray white or white) Put it in the refrigerator (0-4°C) for later use. Under sterile conditions, use the inoculation loop to take 1-2 loops of the original Mucor test tube, inoculate it into the culture medium of a 500ml triangular flask, and plug it with a cotton plug. Vibrate fully to make the strain contact with the culture medium and mix well to make the secondary strain. Mix the bean dregs and rice flour at a ratio of 1:1 and put them in a dry open bottle, about 80-100g. Close the bottle mouth with a clean cotton plug and kraft paper, put it in a steaming pool, and take it out after 60 minutes under normal pressure. , after taking out the abo...

technical field

[0001] The invention relates to a strain of high-temperature-resistant mucormycetes racemosus and its application, in particular to a strain of high-temperature-resistant mucormyces racemosus M-THF-02 and its application, and belongs to the field of microbial fermentation technology application. Background technique

[0002] Mucor ( Mucor ) is a common strain of fermented products such as fermented bean curd and tempeh. It can decompose soybean protein in soybean products, and has the ability to saccharify starch and degrade cellulose. Due to the long-term domestication of mucormycetes under high-protein environmental conditions, it has the ability to synthesize and secrete a variety of extracellular proteases. Enzyme system, pectinase system, etc. form a compound enzyme system to catalyze a variety of biochemical reactions, so that the fermentation product has unique nutritional and health functions.

[0003] The most commonly used mucormycetes in fermente...

Examples

Embodiment 1

[0041] Screening and isolation of thermotolerant Mucor racemosus M-THF-02

[0042] The purpose of the present invention is to isolate the strains of fermented soya bean koji produced by Chengdu Taihefang Brewing Co., Ltd., address: No. 388, Qingxiang Road, Xinfan Town, Xindu District, Chengdu City, Sichuan Province, production batch number: 20181210; isolate a raceme Mucor M-THF-02, and the isolated Mucor racemosa is made into a third-grade strain for application in fermented soybean meal, thereby shortening the fermentation cycle, improving its nutritional content and production efficiency, and ensuring the controllability of the product Sex, thereby reducing the risk of the product.

[0043] 1. Screening of strains

[0044]Add physiological saline one by one from 150 samples for dilution, and select a dilution gradient of 10 -2 、10 -3 Spread the sample dilution on a PDA plate and incubate at 28°C for 48-72 hours, pick and select vigorously growing colonies, and inoculate ...

Embodiment 2

[0078] The application of the industrialized production of Douchi of target Mucor racemosus M-THF-02

[0079] 1. Application method

[0080] (1) Insert Mucorus racemosa and THF-02 for production into PDA slant medium under sterile conditions and culture at 28°C for 48-72 hours, until the slant medium is covered with Mucoral spores (gray white or white) Put it in the refrigerator (0-4°C) for later use. Under sterile conditions, use the inoculation loop to take 1-2 loops of the original Mucor test tube, inoculate it into the culture medium of a 500ml triangular flask, and plug it with a cotton plug. Vibrate fully to make the strain contact with the culture medium and mix well to make the secondary strain. Mix the bean dregs and rice flour at a ratio of 1:1 and put them in a dry open bottle, about 80-100g. Close the bottle mouth with a clean cotton plug and kraft paper, put it in a steaming pool, and take it out after 60 minutes under normal pressure. , after taking out the abo...

Images