A kind of composition of double anti-oxygen and its application and product of double anti-oxygen

Abstract

The invention relates to a composition of oxysugar double antibody and its application and a product of oxyglucose biantibody, belonging to the technical field of chemistry. The study found that the combination of ascorbyl glucoside and α-arbutin has good antioxidant, whitening and moisturizing effects, and the combination of ascorbyl glucoside and α-arbutin can inhibit the generation of fluorescent AGEs and the inhibition rate is as high as 83.92±0.718 %, has a good anti-glycation effect, and has a very high application prospect in the preparation of oxyglucose double antibody products.

Description

technical field

[0001] The invention relates to a composition of oxysugar double antibody, its application and the product of oxyglucose biantibody, and belongs to the technical field of chemistry. Background technique

[0002] Glycation end-products (Advanced Glycation End-products, hereinafter referred to as "AGEs") refer to the free amino groups of proteins, amino acids, lipids or nucleic acids and other macromolecular substances under non-enzymatic conditions. A group of stable end products produced after condensation, rearrangement, cleavage, and oxidative modification, which are brownish-yellow. The process by which AGEs are produced is called non-enzymatic protein glycosylation, which is an irreversible reaction.

[0003] There are two sources of AGEs in the human body, namely endogenous (produced in the body) and exogenous (dietary intake). Endogenous AGEs are produced by the saccharification of carbohydrates and proteins in the human body, while exogenous AGEs ref...

Examples

experiment example 1

[0033] Experimental Example 1: Effects of Different Compounds on the Generation of AGEs (BSA-Fructose Mimic Reaction System)

[0034] 1. Preparation of reagents and sample solutions

[0035] Solvent A: take sodium azide and use phosphate buffer (50 mmol / L, pH 6.5) to prepare a phosphate buffer containing 0.1% (m / m) sodium azide;

[0036] Aminoguanidine solution: take aminoguanidine and use solvent A to prepare an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0037] Fructose solution: take fructose and use solvent A to prepare a fructose solution with a concentration of 1.5 mol / L;

[0038] Bovine serum albumin (BSA) solution: prepare bovine serum albumin with solvent A to obtain a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0039] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside with phosphate buffer (50 mmol / L, pH 6.5) to prepare ascorbyl glucoside solution mother solution with a concentration of 5 mg / mL;

[004...

experiment example 2

[0063] Experimental example 2: Effects of different compounds on the generation of AGEs (BSA-MGO simulated reaction system)

[0064] Specific steps are as follows:

[0065] 1. Preparation of reagents and sample solutions

[0066] Solvent A: take sodium azide and use phosphate buffer (50 mmol / L, pH 6.5) to prepare a phosphate buffer containing 0.1% (m / m) sodium azide;

[0067] Aminoguanidine solution: take aminoguanidine and use solvent A to prepare an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0068] Pyruvaldehyde (MGO) solution: take glyoxal and prepare it with solvent A to obtain a methylglyoxal (MGO) solution with a concentration of 60 mmol / L;

[0069] Bovine serum albumin (BSA) solution: prepare bovine serum albumin with solvent A to obtain a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0070] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside with phosphate buffer (50 mmol / L, pH 6.5) to prepare ascorbyl glu...

Embodiment 1

[0092] Example 1: Oxygen double antibody mask

[0093] The ingredients of the mask liquid attached to the mask contain (100% by total mass):

[0094] Formula: Ascorbyl Glucoside 20%, Vitamin E 2.0%, Butylene Glycol 5.0%, Sodium Hyaluronate 0.5%, Bisabolol 0.4%, Benzoic Acid 0.1%, and the rest is water.

[0095] Preparation method: according to the above formula, weigh the raw materials and mix them evenly, and prepare the facial mask according to the conventional technology in the field.

technical field

[0001] The invention relates to a composition of oxysugar double antibody, its application and the product of oxyglucose biantibody, and belongs to the technical field of chemistry. Background technique

[0002] Glycation end-products (Advanced Glycation End-products, hereinafter referred to as "AGEs") refer to the free amino groups of proteins, amino acids, lipids or nucleic acids and other macromolecular substances under non-enzymatic conditions. A group of stable end products produced after condensation, rearrangement, cleavage, and oxidative modification, which are brownish-yellow. The process by which AGEs are produced is called non-enzymatic protein glycosylation, which is an irreversible reaction.

[0003] There are two sources of AGEs in the human body, namely endogenous (produced in the body) and exogenous (dietary intake). Endogenous AGEs are produced by the saccharification of carbohydrates and proteins in the human body, while exogenous AGEs ref...

Examples

experiment example 1

[0033] Experimental Example 1: Effects of Different Compounds on the Generation of AGEs (BSA-Fructose Mimic Reaction System)

[0034] 1. Preparation of reagents and sample solutions

[0035] Solvent A: take sodium azide and use phosphate buffer (50 mmol / L, pH 6.5) to prepare a phosphate buffer containing 0.1% (m / m) sodium azide;

[0036] Aminoguanidine solution: take aminoguanidine and use solvent A to prepare an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0037] Fructose solution: take fructose and use solvent A to prepare a fructose solution with a concentration of 1.5 mol / L;

[0038] Bovine serum albumin (BSA) solution: prepare bovine serum albumin with solvent A to obtain a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0039] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside with phosphate buffer (50 mmol / L, pH 6.5) to prepare ascorbyl glucoside solution mother solution with a concentration of 5 mg / mL;

[004...

experiment example 2

[0063] Experimental example 2: Effects of different compounds on the generation of AGEs (BSA-MGO simulated reaction system)

[0064] Specific steps are as follows:

[0065] 1. Preparation of reagents and sample solutions

[0066] Solvent A: take sodium azide and use phosphate buffer (50 mmol / L, pH 6.5) to prepare a phosphate buffer containing 0.1% (m / m) sodium azide;

[0067] Aminoguanidine solution: take aminoguanidine and use solvent A to prepare an aminoguanidine solution with a concentration of 0.1 mg / mL;

[0068] Pyruvaldehyde (MGO) solution: take glyoxal and prepare it with solvent A to obtain a methylglyoxal (MGO) solution with a concentration of 60 mmol / L;

[0069] Bovine serum albumin (BSA) solution: prepare bovine serum albumin with solvent A to obtain a bovine serum albumin (BSA) solution with a concentration of 30 mg / mL;

[0070] Ascorbyl glucoside solution mother solution: take ascorbyl glucoside with phosphate buffer (50 mmol / L, pH 6.5) to prepare ascorbyl glu...

Embodiment 1

[0092] Example 1: Oxygen double antibody mask

[0093] The ingredients of the mask liquid attached to the mask contain (100% by total mass):

[0094] Formula: Ascorbyl Glucoside 20%, Vitamin E 2.0%, Butylene Glycol 5.0%, Sodium Hyaluronate 0.5%, Bisabolol 0.4%, Benzoic Acid 0.1%, and the rest is water.

[0095] Preparation method: according to the above formula, weigh the raw materials and mix them evenly, and prepare the facial mask according to the conventional technology in the field.

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