45results about How to "Achieve early detection" patented technology

The invention discloses an ECL (electrochemiluminescence) immunosensor for detecting tumor markers and a preparation method and applications thereof. The immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is a gold electrode, cysteamine, glutaraldehyde, a tumor marker first-antibody, a tumor marker, and a composite functionalized nanosphere are sequentially decorated on the surface of the gold electrode, the composite functionalized nanosphere is simultaneously marked by a tumor marker second-antibody and an ECL marker, and non-specific active sites are closed by using bovine serum albumins. The preparation method comprises the steps of preparing the gold electrode on which the tumor marker first-antibody is fixedly carried; synthesizing the composite functionalized nanosphere simultaneously marked by the tumor marker second-antibody and the ECL marker; and finally, assembling the obtained gold electrode and the composite functionalized nanosphere into an ECL immunosensor. The ECL immunosensor disclosed by the invention is high in sensitivity, rapid in analysis speed, strong in stability and selectivity, good in reproducibility, easy to operate, and flexible in method.

The invention discloses oral cavity comprehensive detecting method and apparatus based on a flexible phase controlled ultrasonic array. The apparatus comprises a display module, a phase controlled ultrasonic transmitting module and a phase controlled ultrasonic receiving module connected with a control module, respectively. The phase controlled ultrasonic transmitting module and the receiving module are further connected with a flexible phased array ultrasonic transducer array. The method comprises the following steps of: performing ultrasonic scanning to detect teeth facing outwards skin in periphery of the oral cavity on the face or in all directions of other soft tissues of the oral cavity through the flexible phased array ultrasonic transducer array; transmitting ultrasonic waves with set frequency to detection points through the phased array focused ultrasound when detecting the teeth so as to detect the amplitude of the reflected wave and detect whether defect waves exist in the reflected wave; and transmitting ultrasounds with different frequencies to the detection points through the phased array focused ultrasound and detecting the attenuation of the transmitted wave with different frequencies at the other end when detecting the soft tissues of the oral cavity so as to obtain broadband ultrasonic attenuation parameters. Therefore, health states of the soft tissues of oral cavity can be obtained rapidly.

The invention discloses mental stress assessment and high-risk population intervention system oriented to undergraduates. The mental stress assessment and high-risk population intervention system comprises a multi-mode brain function detecting device, a mobile terminal, a network server and a monitoring client-side, wherein the multi-mode brain function detecting device is connected with the mobile terminal through a Bluetooth signal, the mobile terminal is connected with the network server through a mobile internet, and the monitoring client-side is connected with the network server through the internet. The mental stress assessment and high-risk population intervention system is based on a mobile internet technology, adopts the multi-mode brain function detecting device to acquire brain blood oxygen signals and electroencephalogram signals of the undergraduates, utilizes the mobile terminal to receive the brain blood oxygen signals and electroencephalogram signals and input family mental disease histories and earlier-stage diagnosis information of the undergraduates, then uploads the data to the network server for data processing and analysis, generates corresponding diagnosis results, feeds the results back to the mobile terminal and the monitoring client-side, achieves early finding and early treatment of the mental diseases of the undergraduates and is beneficial to early warning and intervention of the mental diseases of the undergraduates.

The invention relates to a surface enhanced Raman scattering technology-based composite material and a preparation method thereof. The composite material comprises microspheres modified with first target molecules on the surfaces and a second component modified with Raman signal molecules and second target molecule-modified polymers on the surface. In a solution containing a substance to be detected, the substance to be detected, the microspheres and the second component are bonded to the second target molecules through the first target molecules so that the composite material which can be easily precipitated through centrifugation is obtained. The invention also discloses the preparation method and a use of the composite material. The composite material can be used for detecting a human prostate-specific antigen (PSA), a human ferrohemoglobin (Hb), a procalcitonin (PCT) or disease markers (such as tumor markers, cardiovascular disease markers, senile dementia markers, mycoplasma and chlamydia), can effectively reduce false positive and false negative effects and has advantages of high sensitivity, simpleness, fastness and low cost.

The invention discloses a biomarker relating to renal clear cell carcinoma. The biomarker is SLC17A9. Experiments prove that the expression of the SLC17A9 is up-regulated in patients suffering from the renal clear cell carcinoma, proliferation and invasion of the renal clear cell carcinoma cells can be inhibited, and the SLC17A9 can serve as a diagnosis and/or treatment target to be applied to clinical renal clear cell carcinoma diagnosis and treatment.

The invention relates to the field of protein fingerprint detection technology, especially to a fingerprint model for liver cancer serum characteristic protein detection. Seven abnormal fucosylation glycoproteins which include four up-regulated proteins and three down-regulated proteins are screened from serum as a characteristic proteome. On the base of the characteristic proteome, the fingerprint model for liver cancer serum characteristic protein detection is built. The molecular weights of the four up-regulated proteins are 9290.06 Dalton, 2660.24 Dalton, 2764.18 Dalton and 1862.71 Dalton respectively. The molecular weights of the three down-regulated proteins are 4282.99 Dalton, 3963.52 Dalton and 6023.31 Dalton respectively. The fingerprint model provides a new method for early diagnosis of liver cancer, provides a foundation for further finds of new tumor markers, and is beneficial to improve the accuracy of diagnosis of liver cancer.

The invention discloses a PCR (Polymerase Chain Reaction) specific primer and a kit for detecting a thyroid cancer metastasis associated gene, and a using method of the kit. The thyroid cancer metastasis associated gene related to PCR specific primer and the kit, which are independently researched and developed and are based on a high throughput sequencing technology, for detecting the thyroid cancer metastasis associated gene and the using method of the kit is subjected to sample validation, the PCR specific primer, the kit and the using method can be used for detecting a mutation situation of the thyroid cancer metastasis associated gene, a detection result can be used as an intermediate result together with other detecting results for judging whether cancer cells are metastasized or notor the cancer cells have the possibility of metastasis or recurrence, and an important significance in treatment and prognosis of thyroid cancer is obtained; in addition, limit of a traditional detecting technology is broken through by utilizing the high throughput sequencing technology, the detection sensitivity is increased, and even low-abundance mutant genes can also be detected, so that theimportant significance in aided prediction on spreading and recurrence of thyroid cancer cells is obtained.

The invention discloses a method for simultaneously detecting various intestinal tract viruses, and belongs to the field of biologic detection. The method comprises the steps of respectively designinga pair of specific primers Fs and Rs and general primers Fg and Rg for target genes of intestinal tract virus general type, coxsackie virus A4 types, A6 types, A10 types and A16 types, and intestinaltract virus 71 types; firstly performing enrichment amplification through specific primers Fg+Fs and Rg+Rs with general primer labels; then performing exponential amplification with general primer labels Fg and Rg; and collecting fluorescence signals of exponential amplification products for detection. Through the adoption of the method disclosed by the invention, 6 kinds of intestinal tract virus pathogens of the intestinal tract virus general type, the coxsackie virus A4 types, A6 types, A10 types and A16 types, and the intestinal tract virus 71 types can be identified at a time. The methodis high in specificity and high in sensitivity, realization of early discovery, early diagnose and early treatment of hand-foot-mouth diseases can be facilitated, and besides, emergency disposal andpreventing and controlling capacity for hand-foot-mouth disease epidemic situations can be notably improved.

The invention provides a network prevention-and-treatment system for diabetes and diabetic retinopathy. The system comprises a testing module, a film reading intelligent diagnosis module, an information storage module and an intelligent prevention-and-treatment module. The testing module comprises a blood sugar testing terminal and an eyeground testing terminal and is used for testing the blood sugar concentration of a patient and photographing an eyeground image picture for the patient. The film reading intelligent diagnosis module comprises a film reading unit and an intelligent diagnosis unit and is used for supplying a professional film reading result and a diagnosis result to the eyeground image pictures. A wireless transmission module is used for transmitting blood sugar concentration information and the eyeground image picture. The information storage module is used for storing the blood sugar information and the eyeground image picture. The intelligent prevention-and-treatmentmodule comprises a diabetes prevention-and-treatment unit, a diabetic retinopathy prevention-and-treatment unit and a user terminal and is used for supplying a treatment plan to the patient. The network prevention-and-treatment system settles the problems such as tedious information recording, manpower and time consumption, and insufficient comprehensive diabetic retinopathy picture diagnosis.

The invention belongs to the technical field of cell biology, and particularly discloses a construction method and application of a cell strain for expressing an NTNG1 gene. With liver cancer cells asa target system, the liver cancer cells with stably overexpressed NTNG1 genes are obtained through NTNG1 overexpression of lentiviral infection. With the liver cancer cells as a target system, the liver cancer cells with stably knocked-down NTNG1 gene is obtained through NTNG1 interference of lentiviral infection. Through the application of the cell strain constructed by the invention, the influence of NTNG1 on liver cancer proliferation, growth, apoptosis, invasion, migration and mouse tumorigenesis is studied, an experimental basis is provided for molecular mechanism and prognosis judgmentof liver cancer occurrence and development, and a basis is provided for clinical application.

The invention relates to an optimum mass spectrometry model and a preparation method thereof for detecting the feature protein of liver cancer caused by hepatitis b, belonging to the field of mass spectrometry detection technique. The invention is characterized in that eight up-regulated proteins and three lower-regulated proteins are screened from the blood serum to be used as the feature proteins; any two or more proteins of the eleven proteins are chosen so as to establish a blood serum feature protein mass spectrometry model of identification with two in a group for patients with liver cancer caused by hepatitis b and normal people, and patients with liver cirrhosis caused by hepatitis b, and remote metastasis of liver cancer according to the mass-charge ratio m/z of each protein peak and the critical peak average value of the protein; the preparation method of the invention provides a foundation for discovering new liver cancer biological marks. The method of the invention is better than any single detection method adopted currently for the detection of the liver cancer, and provides a non-invasive technique for the early detection and early treatment of the liver cancer, thus providing a new method for reducing the mortality of the liver cancer, improving the cure rate of the liver cancer and screening and examining the liver cancer for high-risk population further.

The invention provides a pig farm epidemic prevention system and an epidemic prevention method. The pig farm epidemic prevention system comprises a disinfection area, a breeding pig feeding area, a fattening area, an isolation area, signal conversion equipment and a server, and disinfection equipment and drying equipment are arranged in the disinfection area; and a plurality of temperature sensorsare laid on the ground of the breeding pig feeding area and the ground of the fattening area separately, the temperature sensors are connected with the signal conversion equipment, and the signal conversion equipment is connected with the server. According to the pig farm epidemic prevention system, the multiple areas are arranged, pigs in different conditions can be conveniently managed and controlled, and cross infection is prevented; fever pigs are recognized through the temperature sensors and the like, the fever pigs are isolated in time, and early discovery and early control of swine fever are achieved; and through arranging a special channel and the disinfection area, effective prevention and control management is carried out, and major epidemic outbreak is prevented.

The invention discloses primers, a probe, a kit and a method used for detecting porcine circovirus 3 based on the digital PCR technology. The primers and the probe comprise an upstream primer FP, a downstream primer BP and the probe Probe; and the detection kit comprises 2XddPCR Supermix, ultrapure water without RNA anzyme, a virus total DNA extracting reagent, a negative control and a positive control. According to the detection method, DNA of a to-be-detected sample is extracted, quantitative detection is carried out on the DNA of the to-be-detected sample by adopting the microdrop digital PCR technology, whether the to-be-detected sample contains the porcine circovirus 3 or not is judged through analysis on the obtained copy number after amplification, and the content of the porcine circovirus 3 is also determined. The primers, the probe, the kit and the method have the advantages that the operation is accurate and sensitive, the applicability is wide, the dependency on a standard curve is eliminated, the absolute quantification can be realized, the early discovery, early treatment and early prevention for the porcine circovirus 3 virus disease diagnosis can be realized, and thus the outbreak of diseases can be effectively controlled.