The invention provides a seedling culture box for culturing agricultural production seedlings. The box comprises a box body, a box cover, a water tank, a water inlet pipe, a first water pump, a mounting frame, a supporting frame, a seedling culture tank, a water storage tank, a water level sensor, a humidity sensor, a temperature sensor, a guide pipe, a sprayer, a second water pump, a display screen and a switch group and a controller, the supporting frame is mounted in the box body through the mounting frame, the seedling culture tank is arranged in the supporting frame, the guide pipe is mounted in the box body through a pipe fastener, the sprayer is arranged on one side of the guide pipe, the second water pump is mounted in the water tank and connected with the guide pipe, the display screen is mounted on one side of the box body through screws, and the controller is arranged on one side of the display screen. As the seedling culture box is provided with the mounting frame, the seedling culture tank and the sprayer, a structure is conveniently adjusted, seedling culture effects are good, transplanting is more convenient, humidity in the air can be improved, and the survival rateof cultured seedlings is higher.