The invention discloses a fresh pericarpium zanthoxyli 
oleoresin separation and purification process which comprises the following steps: after harvesting fresh pericarpium zanthoxyli, carrying out cold pressing within two hours to obtain fresh pericarpium zanthoxyli 
oleoresin, carrying out countercurrent extraction separation on the fresh pericarpium zanthoxyli 
oleoresin through a liquid substance extraction and separation unit of a supercritical CO2 fluid dynamic countercurrent extraction and 
separation system, and after the countercurrent extraction separation is completed, obtaining fresh pericarpium zanthoxyli resin from the extraction unit, and obtaining fresh pericarpium zanthoxyli 
essential oil through a separation unit. The extraction 
separation process is as follows: the feeding rate is 40kg / h, the CO2 flow rate is 600L / h, the extraction temperature is 45 DEG C, and the extraction pressure is 20MPa; the separation I temperature is 40 DEG C, the separation I pressure is 7.5 MPa, the separation II temperature is 55 DEG C, and the separation II pressure is 4-6 MPa; the total yield of the oleoresin product reaches 98.74%-99.17%, and the separation efficiency of volatile oil reaches 94.00%-99.24%; the volatile 
oil content of the obtained 
essential oil can reach 101.94-128.93 ml / 100g; the content of 
amide substances in the obtained resin can reach 575.22 mg / g to 645.99 mg / g, and the content of the 
amide substances is increased to 79.08% to 98.25%. According to the process disclosed by the invention, the fresh pericarpium zanthoxyli 
essential oil with high volatile 
oil content and the fresh pericarpium zanthoxyli resin with a high 
amide substance content can be obtained, and the purification effect of the two products is remarkable.